Identification of differential expressed PE exosomal miRNA in lung adenocarcinoma, tuberculosis, and other benign lesions

Wang, Yan; Xu, Ye; Zou, Yeqing; Lin, Jen‐Kou; Huang, Bo; Liu, Jing; Li, Jing; Zhang, Jing; Yang, Weiming; Min, Qing‐Hua; Li, Shu-Qi; Gao, Qiu-Fang; Sun, Fan; Chen, Qing-Gen; Zhang, Lei; Jiang, Yulin; Deng, Libin; Wang, Xiaozhong

doi:10.1097/md.0000000000008361

Cited by 59 publications

(54 citation statements)

References 64 publications

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“…The levels of EV miR-451a, miR-194-5p, and miR-486-5p in the plasma were found to be significantly increased in patients with LUAD, compared to the healthy volunteers. In agreement with our observations, several studies have reported that miR-451a, miR-194-5p, and miR-486-5p were dysregulated in plasma/serum from lung cancer patients [25][26][27][28][29][30]. However, the samples used in most of these studies are late-stage LUAD patients.…”

Section: Discussionsupporting

confidence: 92%

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A panel of miRNAs derived from plasma extracellular vesicles as novel diagnostic biomarkers of lung adenocarcinoma

Yao

et al. 2019

FEBS Open Bio

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Lung cancer is the leading cause of cancer‐related morbidity and mortality worldwide, with lung adenocarcinoma (LUAD) being the most common histological subtype (approximately 40%). In the absence of reliable screening biomarkers for early diagnosis, most patients with LUAD are inevitably diagnosed at an advanced stage. MicroRNAs (miRNAs) encapsulated within plasma‐derived extracellular vesicles (EVs) may be suitable for use as noninvasive diagnostic biomarkers for aggressive malignancies, including LUAD. In this study, we first investigated the miRNA profiles of plasma‐derived EVs from LUAD patients and healthy donors, and then systematically evaluated the expression patterns of selected plasma‐derived EV miRNAs in a large cohort of patients with LUAD and healthy controls. Notably, we observed that miR‐451a, miR‐194‐5p, and miR‐486‐5p were significantly increased in EVs from LUAD patients, compared to healthy controls. The area under the curve values for the three miRNAs were 0.9040 (95% confidence interval [CI], 0.8633–0.9447) for miR‐451a, 0.7492 (95% CI, 0.6992–0.7992) for miR‐194‐5p, and 0.9574 (95% CI, 0.9378–0.9769) for miR‐486‐5p, while the AUC of the combination of these three miRNAs was 0.9650. Thus, these results suggest that these EV miRNAs may be promising candidates for the development of highly effective, noninvasive biomarkers for early LUAD diagnosis.

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Section: Discussionsupporting

confidence: 92%

“…Secondly, no benign or symptom similar patients are included in our study. Recently, Wang et al . found miR‐483‐5p in the exosomes derived from pleural effusions was preferentially represented in LUAD when compared to tuberculous and other benign lesions.…”

Section: Discussionmentioning

confidence: 99%

A panel of miRNAs derived from plasma extracellular vesicles as novel diagnostic biomarkers of lung adenocarcinoma

Yao

et al. 2019

FEBS Open Bio

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“…Meanwhile, lnc-AC145676.2.1-6 is able to interact with 45 miRNAs (Supplementary Material, Figure S2), several of which have been reported to correlate with mycobacterial infection, including miR-29a (Siddle et al, 2014;Fu et al, 2011;Sharbati et al, 2011), miR-150 (Ghorpade et al, 2012Wang et al, 2017b), and miR-92a (Wang et al, 2018). Accumulated evidence implies that dysregulated miR-29a is critical to the pathogenesis of TB.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of lnc-AC145676.2.1-6 and lnc-TGS1-1 and their variants in western Chinese tuberculosis patients

Bai

et al. 2019

International Journal of Infectious Diseases

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Objectives: Tuberculosis (TB) remains a global public health problem. Recent studies have implicated long non-coding RNAs and their variants as possibly playing important roles in TB. The aim of this study was to assess the clinical relevance of lnc-AC145676.2.1-6 and lnc-TGS1-1 and their variants in a western Chinese population. Methods: This case-control study included 467 TB patients and 473 healthy controls from West China Hospital. The expression levels of lnc-AC145676.2.1-6 and lnc-TGS1-1 were analyzed by reverse transcriptase quantitative real-time PCR. Single-nucleotide polymorphism genotyping was performed using a custom-designed 2 Â 48-Plex SNPscan kit. Results: It was observed that lnc-AC145676.2.1-6 and lnc-TGS1-1 expression levels were both obviously down-regulated in TB patients. In addition, a lower expression level of lnc-TGS1-1 was associated with the presence of thrombocytopenia in TB patients during anti-TB treatment, and the homozygous CC genotype of rs4737420 correlated with a decreased risk of leukopenia, compared with individuals with the T allele (TT/CT genotype), in the dominant mode. Conclusions: For the first time, potential TB-associated promoting effects were identified for the decreased expression levels of lnc-AC145676.2.1-6 and lnc-TGS-1, while lnc-TGS1-1 and its variant rs4737420 may be predictive indicators of anti-TB drug-induced adverse drug reactions. Larger validation studies on different populations are warranted to confirm these findings.

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“…In a more recent study with a similar design, pleural exosomal miRNAs were divided into 3 groups: lung adenocarcinoma (n = 8), tuberculosis (n = 10), and other benign lesions (n = 4). 21 In this study, only lung adenocarcinoma was considered, whereas in the previous study, no distinction was made among different lung cancer subtypes. Nine miRNAs (miRNA-205-5p, miRNA-483-5p, miRNA-375, miRNA-200c-3p, miRNA-429, miRNA-200b-3p, miR-NA-200a-3p, miRNA-203a-3p, and miRNA-141-3p) had increased expression in lung adenocarcinoma exosomes.…”

Section: Lung Cancermentioning

confidence: 93%

“…They found different expression profiles for each group; in particular, miRNA‐205‐5p and miRNA‐200b were markedly increased only in lung cancer. In a more recent study with a similar design, pleural exosomal miRNAs were divided into 3 groups: lung adenocarcinoma (n = 8), tuberculosis (n = 10), and other benign lesions (n = 4) . In this study, only lung adenocarcinoma was considered, whereas in the previous study, no distinction was made among different lung cancer subtypes.…”

Section: Lung Cancermentioning

confidence: 99%

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

Nicolè

Cappello

Cappellesso

et al. 2019

Cancer Cytopathology

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The cytomorphologic evaluation of serous cavity specimens can quickly determine the cause of an effusion as well as the presence or absence of a neoplastic process. Ancillary tests such as immunohistochemistry and fluorescence in situ hybridization are frequently used to help to definitively identify malignant cells, determine a site of origin, and distinguish between malignant and reactive mesothelial proliferations. In recent years, microRNAs (miRNAs) have been increasingly evaluated in cytologic specimens, including those from serous cavities. The examination of miRNA is attractive because of the stability of miRNA in such specimens and data suggesting that miRNA can provide prognostic and therapeutic information in addition to its role in diagnosis. Furthermore, miRNAs exist within extracellular exosomes, and this allows for their analysis in specimens that contain only rare malignant cells, degenerated cells, or obscuring components. This review discusses the technical aspects of specimen processing for miRNA analysis, recent studies of miRNA expression in malignant serous cavity specimens, and the potential importance of miRNA expression analysis for serous cavity specimens in the near future.

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Identification of differential expressed PE exosomal miRNA in lung adenocarcinoma, tuberculosis, and other benign lesions

Abstract: Supplemental Digital Content is available in the text

Cited by 59 publications

References 64 publications

A panel of miRNAs derived from plasma extracellular vesicles as novel diagnostic biomarkers of lung adenocarcinoma

A panel of miRNAs derived from plasma extracellular vesicles as novel diagnostic biomarkers of lung adenocarcinoma

Clinical significance of lnc-AC145676.2.1-6 and lnc-TGS1-1 and their variants in western Chinese tuberculosis patients

MicroRNA profiling in serous cavity specimens: Diagnostic challenges and new opportunities

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