Identification of Clinically Important Species of Enterococcus Within 1 Day with Randomly Amplified Polymorphic DNA (RAPD)

Quednau, M.; Ahrné, Siv; Petersson, Ann Catherine; Molin, Göran

doi:10.1007/s002849900318

Cited by 54 publications

(36 citation statements)

References 12 publications

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“…faecium at WWTF-1 was 9:1, which is similar to other studies that have surveyed enterococci at meat processing operations (beef, pork and poultry) and from associated meat products. In most of those studies, E. faecalis was dominant followed by E. faecium [14,30,36,45,49,54,58]. In the current study, the species composition among sludge and house flies was comparable with E. faecalis being the most abundant species from both sources (Fig.…”

Section: Discussionmentioning

confidence: 50%

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

2013

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Enterococci are important nosocomial pathogens with Enterococcus faecalis most commonly responsible for human infections. In this study, we used several measures to test the hypothesis that house flies, Musca domestica (L.), acquire and disseminate antibiotic resistant and potentially virulent E.faecalis from wastewater treatment facilities (WWTF) to the surrounding urban environment. House flies and sludge from four WWTF (1-4) as well as house flies from three urban sites close to WWTF-1 were collected and cultured for enterococci. Enterococci were identified, quantified, screened for antibiotic resistance and virulence traits, and assessed for clonality. Of the eleven antibiotics tested, E. faecalis was most commonly resistant to tetracycline, doxycycline, streptomycin, gentamicin and erythromycin and these traits were intra-species horizontally transferrable by in vitro conjugation. Profiles of E. faecalis (prevalence, antibiotic resistance, and virulence traits) from each of WWTF sludge and associated house flies were similar, indicating that flies successfully acquired these bacteria from this substrate. The greatest number of E. faecalis with antibiotic resistance and virulence factors (i.e., gelatinase, cytolysin, enterococcus surface protein, and aggregation substance) originated from WWTF-1 that processed meat waste from a nearby commercial meat processing plant, suggesting an agricultural rather than human clinical source of these isolates. E. faecalis from house flies collected from three sites 0.7 -1.5 km away from WWTF-1 were also similar in their antibiotic resistance profiles; however, antibiotic resistance was significantly less frequent. Clonal diversity assessment using pulsed-field gel electrophoresis revealed the same clones of E. faecalis from sludge and house flies from WWTF-1 but not from the three urban sites close to WWTF-1. This study demonstrates that house flies acquire antibiotic resistant enterococci from WWTF and potentially disseminate them to the surrounding environment.

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Section: Discussionmentioning

confidence: 50%

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

2013

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“…Randomly amplified polymorphic DNA (RAPD) analysis (10,22) was used to group the isolates; temporal temperature gradient gel electrophoresis (TTGE), where the 16S ribosomal DNA (rDNA) molecule is used, was used for identification to the species level (29); and multiplex PCR, where another area of the rDNA, namely, the intergenic spacer region between the 16S and the 23S rDNAs, is used for the identification (27), was also used.…”

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confidence: 99%

Vaginal Lactobacillus Flora of Healthy Swedish Women

et al. 2002

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“…The polymerase chain reaction (PCR) of the samples was run with a 9-mer primer (5?-ACGCGCCCT-3? ; Scandinavian Gene Synthesis, Kö ping, Sweden), as previously described (26). Agarose gel electrophoresis was run; the gels were stained with ethidium bromide and photographed under UV illumination.…”

Section: Randomly Amplified Polymorphic Dna (Rapd) Analysismentioning

confidence: 99%

“…paracasei group to the liver site (three isolates), to the portal blood (two isolates), to the arterial blood (three isolates), to the MLNs (three isolates), from one rat in L. gasseri group to the MLN site (one isolate), from three rats in Bifidobacterium 'urinalis' group to the liver site (10 isolates), to the portal blood (three isolates), to the MLN site (one isolate) and from one rat in B. infantis to the liver site (one isolate). Crude cell extracts were prepared (26). The polymerase chain reaction (PCR) of the samples was run with a 9-mer primer (5?-ACGCGCCCT-3?…”

Section: Randomly Amplified Polymorphic Dna (Rapd) Analysismentioning

confidence: 99%

Probiotic strains ofLactobacillusandBifidobacteriumaffect the translocation and intestinal load ofEnterobacteriaceaedifferently after D-galactosamine-induced liver injury in rats

Osman

Adawi

Ahrné

et al. 2005

Microbial Ecology in Health and Disease

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Department of Surgery, University Hospital Malmö , Lund University, Sweden AbstractIn an acute liver injury model we compared the effects of different Lactobacillus and Bifidobacterium strains on bacterial translocation, intestinal load of Enterobacteriaceae and the extent of liver injury. This was an experimental study carried out in an university hospital in Sweden. Sprague-Dawley rats were divided into six groups: liver injury control and five groups of liver injury with administration of three different Lactobacillus and two Bifidobacterium strains (orally twice daily for 8 days). Liver injury was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body weight). The main outcome measures were samples collected 24 h after injury. Liver enzymes and bilirubin serum levels, bacterial translocation (to arterial and portal blood, liver and mesenteric lymph nodes), intestinal load of Enterobacteriaceae in relation to lactobacilli and the total bacterial load were evaluated and randomly amplified polymorphic DNA (RAPD) analysis of translocating bacteria was carried out. Lactobacillus plantarum DSM 9843, Lactobacillus gasseri 5B3 and Bifidobacterium infantis DSM 15158 decreased bacterial translocation to the liver compared with the liver injury control group. Lactobacillus paracasei DSM 13434 translocated to the liver. The Enterobacteriaceae count in the caecum decreased in the L. plantarum DSM 9843, L. gasseri 5B3, Bifidobacterium 'urinalis' 3B1 and B. infantis DSM 15158 groups, while all the administered probiotics decreased it in the colon. The levels of alanine aminotransferase (ALT) and bilirubin were significantly lower in the L. plantarum DSM 9843 and B. infantis DSM 15158 groups compared with the liver injury control group. All test strains except L. paracasei DSM 13434 inhibited translocation to the liver. Instead, L. paracasei was found in the liver and it also failed to decrease the load of Enterobacteriaceae in caecum. The best strains in protecting the liver during injury were L. plantarum DSM 9843 and B. infantis DSM 15158, as reflected by bilirubin and liver enzymes. Thus, there are major effectual differences between strains/species. In contrast, the phylogenetically most diverse strains, L. plantarum DSM 9843 and B. infantis DSM 15158, exercised the same effects.

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Identification of Clinically Important Species of Enterococcus Within 1 Day with Randomly Amplified Polymorphic DNA (RAPD)

Cited by 54 publications

References 12 publications

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

Vaginal Lactobacillus Flora of Healthy Swedish Women

Probiotic strains ofLactobacillusandBifidobacteriumaffect the translocation and intestinal load ofEnterobacteriaceaedifferently after D-galactosamine-induced liver injury in rats

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