Identification of circulating microRNAs as diagnostic biomarkers for ovarian cancer: A pooled analysis of individual studies

Abstract

Circulating miRNAs, especially the combination of multiple circulating miRNAs, are promising biomarkers for the diagnosis of ovarian cancer. However, further large-scale prospective studies are necessary to validate the applicability of the miRNAs in the early detection of ovarian cancer.

Circulating microRNAs as Reliable Tumor Biomarkers: Opportunities and Challenges Facing Clinical Application

“…Some studies have evaluated the efficacy of CA-125 in OC and found an optimal sensitivity and specificity of 0.74 and 0.83, respectively Goff et al, 2017). In a meta-analysis conducted by Zhou et al (2018), the pooled sensitivity and specificity a panel of circulating miRNAs was 0.76 and 0.81 (95% CI 0.74 -0.87), with a high diagnostic accuracy corresponding to an area under the curve (AUC) of up to 0.85. Thus, the accuracy of circulating miRNAs for the diagnosis of OC may be greater compared with traditional biomarkers.…”

MicroRNAs in ovarian cancer and recent advances in the development of microRNA-based biosensors

“…[121][122][123] Forster resonance energy transfer (FRET) is another optical method of detetion which is based on radiation-less transfer of electronic excitation from a "donor" molecule to an "acceptor" molecule due to dipole-dipole interaction between the two molecules. 124 One of the latest optical platforms for quantifying absolute miRNAs was demonstrated by Qiu et al 125,126 They established a ratiometric and single-step detection assay using isothermal amplification of miR-21, miR-132 and miR-146a based on time-gated FRET (TG-FRET) between Tb donors and dye acceptors which resulted in miRNA assays with single-nucleotide variant specificity and detection limits down to 4.2 ± 0.5 attomoles. 125,126 In actual case, they modified RCA-FRET miRNA assay demonstrated by Wu et al using steady-state detection of two fluorescent dyes as FRET pair.…”

“…124 One of the latest optical platforms for quantifying absolute miRNAs was demonstrated by Qiu et al 125,126 They established a ratiometric and single-step detection assay using isothermal amplification of miR-21, miR-132 and miR-146a based on time-gated FRET (TG-FRET) between Tb donors and dye acceptors which resulted in miRNA assays with single-nucleotide variant specificity and detection limits down to 4.2 ± 0.5 attomoles. 125,126 In actual case, they modified RCA-FRET miRNA assay demonstrated by Wu et al using steady-state detection of two fluorescent dyes as FRET pair. 127 Although their proof of concept study could achieve an LOD as low as 103 aM and steadfast detection above background of 6 fM, the main limitation of this platform is that it was tested on only a small number of samples.…”