Identification of circulating CD10 positive T cells in angioimmunoblastic T-cell lymphoma

Baseggio, Lucile; Berger, Françoise; Morel, Dominique; Mh, Delfau-Larue; Goedert, Ghislaine; Salles, Gilles; Jp, Magaud; Felman, Pascale

doi:10.1038/sj.leu.2404013

Cited by 43 publications

(36 citation statements)

References 24 publications

(31 reference statements)

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“…2 Among these 28 cases, 12 cases have been previously reported ( Table 1, cases 1-12). 7 In addition to the immunological markers routinely used in T-cell lymphoma diagnosis, the expression of ICOS and PD-1 was evaluated by flow cytometry in 15 lymph nodes and 13 peripheral blood samples. These cases have been cryoconserved in fetal calf serum (FCS; Vysis, Voisins le Bretonneux, France) with 10% dimethyl sulfoxide (DMSO; Sigma, St Louis, MO, USA) in liquid nitrogen until use.…”

Section: Samplesmentioning

confidence: 99%

“…Methods for immunophenotype analysis by multiparametric flow cytometer have been previously reported. 7 The flow data were acquired on a BD FACSCanto II cytometer, which was daily calibrated with BD Cytometer Setup and Tracking Beads, and were analyzed with BD FACS DIVA software (all from Becton Dickinson). Fresh blood samples were lyzed using BD FACS (lyse/Wash assistant; Becton Dickinson).…”

Section: Flow Cytometrymentioning

confidence: 99%

“…17 The peripheral blood dissemination is a common feature in angioimmunoblastic T-cell lymphoma, 7,18,19 and we and others demonstrated that the detection of circulating CD10-positive T cells by flow, whatever be the percentage of CD10-positive cells in lymph node and the lymphocyte count, is relevant for angioimmunoblastic T-cell lymphoma diagnosis. 7,19 However, CD10 expression by T cells may be lacking or too faint to allow a firm diagnosis, which justifies the use of additional markers. CXCL13 and BCL6 represent useful and robust angioimmunoblastic T-cell lymphoma markers by immunohistochemistry, 12,13 but they are not routinely available by flow cytometry in contrast to PD-1 and ICOS.…”

mentioning

confidence: 99%

“…The CD10 expression by neoplastic T cells observed in 80-90% of angioimmunoblastic T-cell lymphoma cases currently represents a specific diagnostic marker that has been demonstrated in several studies using immunohistochemistry as well as flow cytometry. [4][5][6][7][8][9] The gene expression profiling analysis has demonstrated the cellular derivation of angioimmunoblastic T-cell lymphoma from follicular helper T cells (T FH cells), 10,11 that was initially suspected on the expression of T FH markers by immunohistochemistry, particularly the expression of the cytoplasmic CXCL13 chemokine. 12,13 Then, additional T FH markers, including the cell surface molecules CXCR5, the programmed death-1 (PD-1), cytoplasmic SAP (SLAM-associated protein) and the transcription factor BCL6, have been reported as angioimmunoblastic T-cell lymphoma markers by immunohistochemistry.…”

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confidence: 99%

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CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

et al. 2011

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Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (T FH ) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This T FH profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node (n ¼ 15) as well as in peripheral blood (n ¼ 13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia ¼ 10, peripheral blood of healthy donors ¼ 15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.

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Section: Samplesmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

et al. 2011

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“…Testing for CD94, CD161, and killer-cell immunoglobulin-like receptors was not helpful in our cases of AITL, in contrast to the diagnostic utility for clones of large granular lymphocytes in the blood (12). Increased CD10 has been described on T cells in 80% of AITL (5,(13)(14)(15), but nonneoplastic CD10-positive FHT may be increased also in lymph node biopsies with reactive hyperplasia and with B-cell lymphoma (6,7).…”

Section: Vbeta Analysis By Flow Cytometry Is a New Techniquementioning

confidence: 49%

Abnormal immunophenotype of the T‐cell‐receptor beta Chain in follicular‐helper T cells of angioimmunoblastic T‐cell lymphoma

Mao

Surowiecka

Linden

et al. 2015

Cytometry Part B Clinical

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Objective: Analysis for 24 variable regions of the T-cell-receptor beta chain by flow cytometry (Vbeta) is a new technique to detect clonal alpha-beta T lymphocytes and is characteristically performed on peripheral blood. Angioimmunoblastic T-cell lymphoma (AITL) has increased neoplastic follicular-helper T cells (FHT), which often express CD10; but nonneoplastic, CD10-positive T cells may be associated with reactive lymphadenopathy and with B-cell lymphomas. This study documents the utility of Vbeta analysis of FHT in specimens of AITL from blood, from marrow, and from lymph nodes.Methods: The electronic medical record in the flow cytometry laboratory was searched for specimens that were analyzed by flow cytometry for Vbeta and that were involved by AITL. Flow cytometry was performed for the following antigens: T-cell-associated proteins, CD10, CD56, CD94, CD161, killer-cell immunoglobulin-like receptors, alpha-beta T-cell receptor, gamma-delta T-cell receptor, and Vbeta.Results: Five patients had six specimens of blood (two), of bone marrow (one), or of lymph nodes (three). Immunophenotypic aberrances were detected for antigens: CD2 (2/6), CD3 (6/6), CD4 (1/6), CD5 (1/6), CD7 (5/6), and CD45 (2/6). All abnormal T-cell populations expressed CD4, and most expressed CD10 (5/6). Four specimens were clonally restricted for Vbeta. Two specimens lacked the alpha-beta Tcell receptor and Vbeta.Conclusions: Vbeta analysis by flow cytometry can be used to detect clonal alpha-beta FHT in AITL, which may be difficult to diagnose with early involvement. Abnormal Vbeta expression on CD10-positive T cells confirms that FHT are the neoplastic cells. V C 2015 International Clinical Cytometry Society

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Chronic Lymphoid Leukaemias and Exfoliating Lymphoma

Leach

Drummond

Doig

2013

Practical Flow Cytometry in Haematology Diagnosis

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Identification of circulating CD10 positive T cells in angioimmunoblastic T-cell lymphoma

Cited by 43 publications

References 24 publications

CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

Abnormal immunophenotype of the T‐cell‐receptor beta Chain in follicular‐helper T cells of angioimmunoblastic T‐cell lymphoma

Chronic Lymphoid Leukaemias and Exfoliating Lymphoma

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