Identification of circular RNAs with host gene-independent expression in human model systems for cardiac differentiation and disease

Siede, Dominik; Rapti, Kleopatra; Górska, Agnieszka; Katus, Hugo A.; Altmüller, Janine; Boeckel, Jes‐Niels; Meder, Benjamin; Maack, Christoph; Völkers, Mirko; Müller, Oliver; Backs, Johannes; Dieterich, Christoph

doi:10.1016/j.yjmcc.2017.06.015

Cited by 105 publications

(104 citation statements)

References 29 publications

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“…The expression of circRNAs has been appropriately correlated with an abundance of host genes in different animal tissues [52][53][54][55] , such as oar_circ_0003451 and TTN, and oar_circ_0005250 and MYH7 may play important roles in muscle development and growth 22 ; circRNA8077 and CRIM1, as well as circRNA3314 and TMEM159 play vital roles in the development of the receptive endometrium 56 . The host genes of circRNAs are involved in regulating hair traits, and these circRNAs may be considered as a possible factor regulating cashmere fineness.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq)

Zheng

Hui

Chang

et al. 2020

Sci Rep

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circular RnA (circRnA) is endogenous non-coding RnA (ncRnA) with a covalently closed circular structure. it is mainly generated through RnA alternative splicing or back-splicing. circRnA is known in the majority of eukaryotes and very stable. However, knowledge of the circRnA involved in regulating cashmere fineness is limited. Skin samples were collected from Liaoning cashmere goats (LCG) and Inner Mongolia cashmere goats (MCG) during the anagen period. For differentially expressed circRNAs, RNA sequencing was performed, and the analysis led to an identification of 17 up-regulated circRNAs and 15 down-regulated circRNAs in LCG compared with MCG skin samples. In order to find the differentially expressed circRNAs in LCG, we carried out qPCRs on 10 candidate circRNAs in coarse type skin of LCG (CT-LCG) and fine type skin of LCG (FT-LCG). Four circRNAs: ciRNA128, circRNA6854, circRNA4154 and circRNA3620 were confirmed to be significantly differential expression in LCG. Also, a regulatory network of circRNAs-miRNAs was bioinformatically deduced and may help to understand molecular mechanisms of potential circRnA involvement in regulating cashmere fineness.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq)

Zheng

Hui

Chang

et al. 2020

Sci Rep

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“…Some circRNAs can act as miRNA sponges, which increase the complexity of RNA regulatory networks and play a role in gene expression, or as exonic circRNAs, which can regulate the transcription of their host genes . Circular RNA expression is cell type and tissue specific and can be largely independent of the expression level of the linear host gene . GO analysis was performed to further annotate the biological functions of the host genes of the differentially expressed circRNAs.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive circRNA expression profile and construction of circRNA‐associated ceRNA network in fur skin

Zhu

Liu

et al. 2018

Experimental Dermatology

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Circular RNA (circRNA), a class of non-coding RNAs, is a new group of RNAs that are related to tumorigenesis. The role of circRNAs in various diseases has been already highlighted. However, the expression levels and functions of circRNAs related to the melanocytes in the skin are poorly understood. RNA sequence was performed to analyse the expression profiles of circRNAs in black fur skin and white fur skin during different differentiation stages and investigate the relevant metabolism mechanisms. Differentially expressed circRNAs were detected using empirical Bayes sequencing (EBSeq) and then verified through the quantitative real-time PCR method. The EQSeq analysis of circRNAs identified 11 downregulated and 32 upregulated circRNAs in the embryo of black fur skin and white fur skin, as well as 21 downregulated and 17 upregulated circRNA in the postnatal stage. A circRNA-microRNA (miRNA)-messenger RNA (mRNA) network was established to predict the circRNA targets. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were applied to enrich the mRNA data further. Results showed that the specific mRNAs mainly involved in the transcription-related GOs, especially GO:0042802, GO:0005080 and GO:0032403, demonstrate their specific actions in transcriptional regulation. In the circRNA-miRNA-mRNA network, the most enriched GO terms of the mRNAs were pigmentation, protein autophosphorylation and protein complex. Therefore, the circRNA-miRNA-mRNA pathway may reveal novel mechanisms for pigmentation, and circRNAs may serve as candidates in pigmentation.

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“…Dilated cardiomyopathy is a morphological subtype of this disease. Siede et al (2017) PeerJ reviewing PDF | (2019:01:34077:2:0:NEW 16 Mar 2019)…”

Section: Cardiomyopathymentioning

confidence: 99%

Peer Review #2 of "Circular RNAs as potential biomarkers and therapeutics for cardiovascular disease (v0.1)"

2019

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Circular RNAs (circRNAs) are genetic regulators that were earlier considered as "junk." In contrast to linear RNAs, they have covalently linked ends with no poly-adenylated tails. CircRNAs can act as RNA-binding proteins, sequestering agents, transcriptional regulators, as well as microRNA sponges. In addition, it is reported that some selected circRNAs are transformed into functional proteins. These RNA molecules always circularize through covalent bonds, and their presence has been demonstrated across species. They are usually abundant and stable as well as evolutionarily conserved in tissues(liver, lung, stomach), saliva, exosomes, and blood. Therefore, they have been proposed as the "next big thing" in molecular biomarkers for several diseases, particularly in cancer. Recently, circRNAs have been investigated in cardiovascular diseases (CVD) and reported to play important roles in heart failure, coronary artery disease, and myocardial infarction. Here, we review the recent literature and discuss the impact and the diagnostic and prognostic values of circRNAs in CVD.

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Identification of circular RNAs with host gene-independent expression in human model systems for cardiac differentiation and disease

Cited by 105 publications

References 29 publications

Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq)

Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq)

Comprehensive circRNA expression profile and construction of circRNA‐associated ceRNA network in fur skin

Peer Review #2 of "Circular RNAs as potential biomarkers and therapeutics for cardiovascular disease (v0.1)"

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