Identification of circular RNAs and their alterations involved in developing male Xenopus laevis chronically exposed to atrazine

Sai, Linlin; Li, Ling; Hu, Chenyang; Qu, Binpeng; Guo, Qiming; Jia, Qiang; Zhang, Yu; Bo, Cunxiang; Li, Xiangxin; Shao, Hua; Ng, J. C.; Peng, Cheng

doi:10.1016/j.chemosphere.2018.02.140

Cited by 32 publications

(17 citation statements)

References 25 publications

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“…A significant reduction of gonad weight and gonadosomatic index, irregular shape of seminiferous lobules and large empty spaces in testis tissues were found. Meanwhile, AZ can differentially induce gene and circular RNA (circRNA) expression of gonads in male X. laevis tadpoles chronically exposed to 100 μg/L AZ . These results indicated that AZ may affect reproductive and immune systems by interference with the related gene expression in the male X. laevis development.…”

Section: Introductionmentioning

confidence: 89%

“…Total RNAs from individual testis was isolated using Trizol (Invitrogen, Carlsbad, CA, USA). The detailed extraction method of total RNAs was referred in our previous work . The integrity of RNA was determined by denaturing gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

“…The methods of construction of RNA libraries and the harvested of paired‐end reads were described in our previous study . Paired‐end reads were quality controlled by Q30.…”

Section: Methodsmentioning

confidence: 99%

“…Growing evidence suggests that lncRNAs function as miRNA sponges or competing endogenous RNAs (ceRNAs), reducing the availability of miRNAs for mRNA target binding . Our previous work on differentially gene expression (DGEs) and circRNA by AZ suggested that the genes in Wnt signaling pathway and progesterone‐mediated oocyte maturation may participate in the testis development of X. laevis . However, whether the ceRNA network is involved in the abnormal testis development of X. laevis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of long non‐coding RNA involved in atrazine‐induced testicular degeneration of Xenopus laevis

Sai¹,

Qu²,

Zhang³

et al. 2019

Environmental Toxicology

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Long non-coding RNA (lncRNA) plays a critical role in male germline development. Atrazine (AZ) as an environmental endocrine disrupting chemical (EDCs) can induce male reproductive toxicity in amphibians. Our previous studies demonstrated that AZ can alter gene and circular RNA (cir-cRNA) expression of damaged testes in Xenopus laevis (X. laevis). We furthered to investigate the lncRNA expression profiling in the testis of X. laevis. Over 3559 lncRNAs were detected by lncRNA sequencing. AZ induced 40 upregulated and 46 downregulated differentially expressed lncRNAs. KEGG analysis showed that AZ-affected lncRNAs mainly involve in 19 pathways among which 12 pathways are found in circRNA analysis. This study for the first time demonstrated that AZ can alter lncRNAs which may play a role in testicular degeneration through regulating expressions of functional genes in X. laevis. Our data may provide more insights on the mechanism about male reproductive toxicity of EDCs. K E Y W O R D S ceRNA, frog, herbicide, lncRNA, reproductive toxicology

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Section: Introductionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

“…The methods of construction of RNA libraries and the harvested of paired‐end reads were described in our previous study . Paired‐end reads were quality controlled by Q30.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of long non‐coding RNA involved in atrazine‐induced testicular degeneration of Xenopus laevis

Sai¹,

Qu²,

Zhang³

et al. 2019

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

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“…Increasing evidence has indicated potential roles of circRNAs involving diverse mechanisms of action in physiological and pathological conditions such as atherogenesis modulation, cancer development, innate immune response, drug toxicity, and degenerative disc disease (Holdt et al ; Guarnerio et al ; Chen et al ; Sai et al ; Cheng et al ). Some circRNAs have been hypothesized to function as ‘sponges’ to sequester microRNAs (miRNAs), thus preventing interactions between miRNAs and messenger RNAs (mRNAs) (Hansen et al ; Memczak et al ; Zheng et al ; Han et al ).…”

mentioning

confidence: 99%

Current status and potential role of circular RNAs in neurological disorders

Yang

Wang

et al. 2019

Journal of Neurochemistry

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Given the importance of non‐coding RNAs in modulating normal brain functions and their implications in the treatment of neurological disorders, non‐coding RNA‐based diagnostic and therapeutic strategies have shown great clinical potential. Circular RNAs (circRNAs) have emerged as potentially important players in this field. Recent studies have indicated that circRNAs might play vital roles in Alzheimer’s disease, Parkinson’s disease, ischemic brain injury, and neurotoxicity. However, the mechanisms of action of circRNAs have not been fully characterized. We aimed to review recent advances in circRNA research in the brain to provide new insights on the roles of circRNAs in neurological disorders.

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The role of environmental exposures and the epigenome in health and disease

Perera

Faulk

Svoboda

et al. 2019

Environ and Mol Mutagen

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The genetic material of every organism exists within the context of regulatory networks that govern gene expression, collectively called the epigenome. Epigenetics has taken center stage in the study of diseases such as cancer and diabetes, but its integration into the field of environmental health is still emerging. As the Environmental Mutagenesis and Genomics Society (EMGS) celebrates its 50 th Anniversary this year, we have come together to review and summarize the seminal advances in the field of environmental epigenomics. Specifically, we focus on the role epigenetics may play in multi and transgenerational transmission of environmentally induced health effects. We also summarize state of the art techniques for evaluating the epigenome, environmental epigenetic analysis, and the emerging field of epigenome editing. Finally, we evaluate transposon epigenetics as they relate to environmental exposures and explore the role of noncoding RNA as biomarkers of environmental exposures. While the field has advanced over the past several decades, including being recognized by EMGS with its own Special Interest Group, recently renamed Epigenomics, we are excited about the opportunities for environmental epigenetic science in the next 50 years.

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Identification of circular RNAs and their alterations involved in developing male Xenopus laevis chronically exposed to atrazine

Cited by 32 publications

References 25 publications

Analysis of long non‐coding RNA involved in atrazine‐induced testicular degeneration of Xenopus laevis

Analysis of long non‐coding RNA involved in atrazine‐induced testicular degeneration of Xenopus laevis

Current status and potential role of circular RNAs in neurological disorders

The role of environmental exposures and the epigenome in health and disease

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