Identification of Chromosomes Associated with Dental Caries Susceptibility Using Quantitative Trait Locus Analysis in Mice

Abstract

Dental caries is a multifactorial, infectious disease with little known about the host genetic factors influencing susceptibility. This study aimed to identify the major candidate chromosomes for dental caries susceptibility and to detect the relevant regions within these. Quantitative trait locus (QTL) analysis was performed on genetic crosses of C3H/HeJ (caries-resistant) and C57BL/6J (caries-susceptible) mice inoculated with Streptococcus mutans serotype c. In a genomewide scan, three suggestive QTLs were detected on chromosomes 1, 2, and 7, one significant QTL was found on chromosome 2, and one highly significant QTL was detected on chromosome 8. The likelihood ratio statistic (LRS) was raised around the marker D1Mit21 in the middle region of chromosome 1, between D2Mit255 and D2Mit311 in the distal region of chromosome 2, and the region distal to D7Mit31 on chromosome 7. A significant QTL was located between the markers D2Mit237 and D2Mit101 on chromosome 2. The LRS was highly significantly raised between markers D8Mit208 and D8Mit280 on chromosome 8, and exceeded a highly significant level between markers D8Mit211 and D8Mit280. These results suggest that major gene(s) responsible for dental caries susceptibility or resistance are located in one or more of these regions.

Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population

“…Indeed, 40-65% of caries risk has been attributed to this factor (Bretz et al, 2005). Recent studies have confirmed that the caries susceptibility of a patient may be determined by their DNA (Burgner et al, 2006;Patir et al, 2008;Peres et al, 2010;Werneck et al, 2010;Kang et al, 2011;Stanley et al, 2014), in addition to the well-known environmental factors involved in caries risk such as bacteria, diet, oral hygiene, and host factors (Lenander-Lumikari and Loimaranta, 2000;Nariyama et al, 2004;Zero, 2004).…”

A Mouse Caries Model and Evaluation of <i>Aqp5–/–</i> Knockout Mice

“…Variability in caries scores between different strains of mice is thought to be related to differences in caries susceptibility [Kurihara et al, 1991]. In fact, the greater caries scores observed in C57BL/6J and BALB/cJ mice, compared to the C3H/HeJ strain, are the basis of genetic mapping studies to identify candidate genes that function in caries susceptibility [Nariyama et al, 2004;Suzuki and Kurihara, 1998]. Relevant to this study, mice in which a candidate gene is targeted for deletion will eventually require direct testing in caries experiments.…”

Genetic polymorphisms in the carbonic anhydrase VI gene and dental caries susceptibility

“…Studies of twins (Shuler, 2001), families and animal breeding (Hunt, 1944;Klein, 1946), and genomics (Shelling and Ferguson, 2007) indicated that dental caries has an important genetic component and that 40-65% of caries risk may be related to genetic factors (Bretz et al, 2005). It has been widely established that dental caries is mostly influenced by environmental factors such as microbial, diet, oral hygiene, and host aspects (LenanderLumikari et al, 2000;Petersen, 2003;Nariyama et al, 2004); however, there is increasing evidence for a genetic component in caries susceptibility (Patir et al, 2008;Azevedo et al, 2010;Ozturk et al, 2010;Kang et al, 2011). Therefore, genes may have an important role in the initiation and progression of dental caries.…”