Identification of cholelithogenic enterohepatic Helicobacter species and their role in murine cholesterol gallstone formation

Maurer, Kirk J.; Ihrig, Melanie; Rogers, Arlin B.; Ng, Vivian; Bouchard, Guylaine; Leonard, Monika R.; Carey, Martin C.; Fox, James G.

doi:10.1053/j.gastro.2005.01.008

Cited by 144 publications

(137 citation statements)

References 45 publications

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…It is a similar prevalence when compared to data from the Chilean and Asian population, higher than previous data from our environment 4,9,10,15 . Perhaps the findings may be related to the fact that the population studied includes only patients with chronic inflammatory processes, which further selected the sample.…”

Section: Discussionsupporting

confidence: 60%

“…From reports in literature 9,15,16 referring about the isolation of helicobacter species in bile from the liver and particularly of the gallbladder and its implications in the pathogenesis of diseases, we became interested to assess the prevalence of Helicobacter spp in patients operated on for chronic cholecystitis .…”

Section: Discussionmentioning

confidence: 99%

“…The stone formation depends on epidemiological, physical, chemical, nutritional, genetic, immunological and infectious factors to occur 9,10 . This last factor has been studied in population where the cholecystholithiasis and intra-hepatic lithiasis are common diseases and the infection by Helicobacter spp very prevalent, as in Chile, Japan, China and Thailand 4,9,10 .…”

Section: Introductionmentioning

confidence: 99%

“…This last factor has been studied in population where the cholecystholithiasis and intra-hepatic lithiasis are common diseases and the infection by Helicobacter spp very prevalent, as in Chile, Japan, China and Thailand 4,9,10 . The condition most common predisposing for gallbladder's cancer is gallstones 11 .…”

Section: Introductionmentioning

confidence: 99%

“…The condition most common predisposing for gallbladder's cancer is gallstones 11 . Histological changes, considered pre-neoplastic, were demonstrated in the mucosa of gallbladder, limited to mice infected with Helicobacter spp such as, intestinal metaplasia, hyperplasia, dysplasia in addition to eosinophilic inflammation and hyalinosis 9 . In the histology of tumors of the gallbladder, the inflammatory process is commonly seen 12 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder

et al. 2010

View full text Add to dashboard Cite

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder 1Prevalência do Helicobacter spp na colecistite crônica calculosa e correlação com as alterações histológicas da vesícula biliar ABSTRACT Purpose: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings. Methods: 100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCR and Giemsa were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05). Results: Of 68 patients, 42 (61.8%) were PCR for Helicobacter spp and 19 (27.9%) had Giemsa . There was no correlation between the two findings. The PCR for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa (p = 0.025 and p= 0.039). Conclusion: There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.

show abstract

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%