Identification of characteristics in blood and semen stains — A review

Oepen, I

doi:10.1016/0379-0738(88)90142-9

Cited by 7 publications

(8 citation statements)

References 33 publications

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“…Most of the studies on fluorescence of biological fluids and stains have been conducted using inert substrates and there has been very little emphasis on the usefulness or otherwise of the technique on the living 11 . The aim of this study was to identify a technology (if any) suitable for a cheap compact ALS that could be carried in the field by forensic medical officers who perform sexual assault examinations.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent identification of biological and other stains on skin by the use of alternative light sources

Wawryk

Odell

2005

Journal of Clinical Forensic Medicine

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Section: Introductionmentioning

confidence: 99%

Fluorescent identification of biological and other stains on skin by the use of alternative light sources

Wawryk

Odell

2005

Journal of Clinical Forensic Medicine

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“…Then it is often classified with regard to sex, age, fetal blood, menstrual blood, various blood groups, etc. as summarized by Lee [15] and Oepen [16]. In some cases, stains submitted for examination may be very small and procedures require speed, simplicity, reliability and sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography

et al. 1991

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A method using reverse-phase high-performance liquid chromatography (HPLC) for the identification of fetal hemoglobin (Hb F) and the simultaneous estimation of bloodstain age is described. Umbilical cord and neonatal bloodstains can be differentiated from adult stains by the presence of gamma-globin chains which are characteristic of Hb F. With this method, cord and neonatal blood could be distinguished from adult blood in stains up to 32 weeks old. The age of the stain was estimated from the ratio of the peak area of the alpha-globin chain to that of heme on the same chromatogram. The ratio decreased gradually with an increase in the age of the stain up to 20 weeks old. Studies performed at each time period revealed no significant difference in the ratios of cord and neonatal bloodstains or in the ratios of cord and adult stains. The regression equation calculated from the ratios (y) and the ages of stains in weeks (x) expressed logarithmically is y = 2.5758-0.2497 In (x) and the coefficient of correlation is -0.7491 (n = 252, P less than 0.001). The present method, having the advantages of simplicity, speed and sensitivity, should be of great value to forensic science.

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“…In medico-legal practice the age or the amount of blood stain initially present is occasionally uncertain [16]. Also, the amount of Hb extractable gradually decreases with time.…”

Section: Discussionmentioning

confidence: 99%

Identification of fetal hemoglobin in blood stains by high performance liquid chromatography

et al. 1989

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A new method for the identification of fetal hemoglobin (Hb F) in blood stains by reverse-phase high performance liquid chromatography is described. Differentiation between fetal and adult blood stains is based on the existence of gamma-chain peaks which are characteristics of Hb F. Very few gamma chains appeared on chromatograms of all the adult blood stains examined. The level of Hb F could be determined by measuring the total of chromatogram gamma-globin chain areas, and expressing it as a percentage of total Hb. Levels in six cord blood stains on filter paper ranged from 81.1% to 91.3% and remained constant for at least 12 weeks. This method is of great value for its simplicity, sensitivity and speed, and most importantly for its reliability in the field of forensic medicine.

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Identification of characteristics in blood and semen stains — A review

Cited by 7 publications

References 33 publications

Fluorescent identification of biological and other stains on skin by the use of alternative light sources

Fluorescent identification of biological and other stains on skin by the use of alternative light sources

Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography

Identification of fetal hemoglobin in blood stains by high performance liquid chromatography

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