Identification of CD177 as the most dysregulated parameter in a microarray study of purified neutrophils from septic shock patients

Demaret, Julie; Venet, Fabienne; Plassais, Jonathan; Cazalis, Marie-Angélique; Vallin, Hélène; Friggeri, Arnaud; Lepape, Alain; Rimmelé, Thomas; Textoris, Julien; Monneret, Guillaume

doi:10.1016/j.imlet.2016.08.011

Cited by 56 publications

(58 citation statements)

References 49 publications

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“…Our recent work has demonstrated that the percentage of CD177 + neutrophils is significantly increased in peripheral blood and inflamed mucosa of patients with active IBD compared with healthy controls . Immunohistochemistry and quantitative reverse transcription polymerase chain reaction (qRT‐PCR) analysis also revealed that expression of CD177 at mRNA and protein levels was highly increased in peripheral neutrophils and inflamed mucosa of IBD patients compared with that of healthy donors, which is consistent with the previous study showing that CD177 expression was significantly increased in neutrophils from patients with septic shock . Interestingly, we found that the levels of CD177 + neutrophils in inflamed mucosa from patients with active Crohn's disease and UC were positively correlated with Crohn's disease activity index (CDAI) and the Mayo index, respectively.…”

Section: A New Subset Of Neutrophils Cd177+ Neutrophilssupporting

confidence: 90%

Potential roles of neutrophils in regulating intestinal mucosal inflammation of inflammatory bowel disease

Zhou

Liu

2017

J of Digest Diseases

145

112

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Inflammatory bowel diseases (IBD), comprising of ulcerative colitis and Crohn's disease, are inflammatory disorders of the gastrointestinal tract characterized by chronically relapsing mucosal inflammation. Neutrophils, as the effector cells of acute inflammation, have long been reported to play a role in the maintenance of intestinal homeostasis and pathogenesis of IBD. At the early stage of mucosal inflammation in patients with IBD, neutrophils flood into intestinal mucosa, phagocytose pathogenic microbes, and promote mucosal healing and resolution of inflammation. However, large numbers of neutrophils infiltrating in the inflamed mucosa and accumulating in the epithelia cause damage of mucosal architecture, compromised epithelial barrier and production of inflammatory mediators. In this review we discuss the critical roles of neutrophils in modulating innate and adaptive immune responses in intestinal mucosa, and, importantly, clarify the potential roles of neutrophils related to their production of inflammatory mediators, transenthothelial and transepithelial migration into intestinal mucosa, and the underlying mechanisms in regulating mucosal inflammation of IBD. Moreover, we also describe a new subset of neutrophils (i.e., CD177 neutrophils) and illustrate its protective role in modulating intestinal mucosal immune responses in IBD.

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Section: A New Subset Of Neutrophils Cd177+ Neutrophilssupporting

confidence: 90%

Potential roles of neutrophils in regulating intestinal mucosal inflammation of inflammatory bowel disease

Zhou

Liu

2017

J of Digest Diseases

145

112

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“…A previous microarray study of purified neutrophils from patients with septic shock revealed CD177 mRNA has the highest differential expression between cases and controls. 32 Consistent with our data, the study also demonstrated increased expression of ARG1, ANXA3, CKAP4, IL1R2, MMP9 and VNN1. ANXA3 promotes endothelial cell junction integrity in animal models, and endothelial cell motility in vitro.…”

Section: Discussionsupporting

confidence: 92%

Peripheral blood RNA gene expression in children with pneumococcal meningitis: a prospective case–control study

Kulohoma

Marriage

Vasieva

et al. 2017

bmjpo

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IntroductionInvasive pneumococcal disease (IPD), caused by Streptococcus pneumoniae, is a leading cause of pneumonia, meningitis and septicaemia worldwide, with increased morbidity and mortality in HIV-infected children.ObjectivesWe aimed to compare peripheral blood expression profiles between HIV-infected and uninfected children with pneumococcal meningitis and controls, and between survivors and non-survivors, in order to provide insight into the host inflammatory response leading to poorer outcomes.Design and settingProspective case–control observational study in a tertiary hospital in MalawiParticipantsChildren aged 2 months to 16 years with pneumococcal meningitis or pneumonia.MethodsWe used the human genome HGU133A Affymetrix array to explore differences in gene expression between cases with pneumococcal meningitis (n=12) and controls, and between HIV-infected and uninfected cases, and validated gene expression profiles for 34 genes using real-time quantitative PCR (RT-qPCR) in an independent set of cases with IPD (n=229) and controls (n=13). Pathway analysis was used to explore genes differentially expressed.ResultsIrrespective of underlying HIV infection, cases showed significant upregulation compared with controls of the following: S100 calcium-binding protein A12 (S100A12); vanin-1 (VNN1); arginase, liver (ARG1); matrix metallopeptidase 9 (MMP9); annexin A3 (ANXA3); interleukin 1 receptor, type II (IL1R2); CD177 molecule (CD177); endocytic adaptor protein (NUMB) and S100 calcium-binding protein A9 (S100A9), cytoskeleton-associated protein 4 (CKAP4); and glycogenin 1 (GYG1). RT-qPCR confirmed differential expression in keeping with microarray results. There was no differential gene expression in HIV-infected compared with HIV-uninfected cases, but there was significant upregulation of folate receptor 3 (FOLR3), S100A12 in survivors compared with non-survivors.ConclusionChildren with IPD demonstrated increased expression in genes regulating immune activation, oxidative stress, leucocyte adhesion and migration, arginine metabolism, and glucocorticoid receptor signalling.

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“…Our study found that CD177 was the most sensitive to both the Pao 2 /Fio 2 ratio and the viral load in LRT. CD177 (NB-1) is a glycophosphatidylinositol-anchored protein, which is responsible for mediating the migration, degranulation, and superoxide generation by neutrophils during inflammation [ 31 , 32 ]. Activation of matrix metalloproteinase (MMP) 8, 9, and 25 were also found during the acute phase of H7N9 infection.…”

Section: Discussionmentioning

confidence: 99%

Clinical Correlations of Transcriptional Profile in Patients Infected With Avian Influenza H7N9 Virus

Guan

Yang

Wu³

et al. 2018

The Journal of Infectious Diseases

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Identification of CD177 as the most dysregulated parameter in a microarray study of purified neutrophils from septic shock patients

Cited by 56 publications

References 49 publications

Potential roles of neutrophils in regulating intestinal mucosal inflammation of inflammatory bowel disease

Potential roles of neutrophils in regulating intestinal mucosal inflammation of inflammatory bowel disease

Peripheral blood RNA gene expression in children with pneumococcal meningitis: a prospective case–control study

Clinical Correlations of Transcriptional Profile in Patients Infected With Avian Influenza H7N9 Virus

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