Clinical Correlations of Transcriptional Profile in Patients Infected With Avian Influenza H7N9 Virus

Guan, Weijun; Yang, Zifeng; Wu, Nicholas C.; Lee, Horace Hok Yeung; Li, Yimin; Jiang, Wenxin; Shen, Lihan; Wu, Douglas C.; Chen, Rongchang; Zhong, Nanshan; Wilson, Ian A.; Peiris, Malik; Mok, Chris Ka Pun

doi:10.1093/infdis/jiy317

Cited by 17 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Robust multi-factorial immune responses can be elicited against viral infection, such as avian H7N9 disease 17,18 . A recent report has found effective immune responses from a non-severe COVID-19 patient 19 .…”

mentioning

confidence: 99%

Single-cell analysis of severe COVID-19 patients reveals a monocyte-driven inflammatory storm attenuated by Tocilizumab

Guo

et al. 2020

Preprint

View full text Add to dashboard Cite

1 inflammatory storm incited by monocyte centric immune interactions 2 revealed by single-cell analysis 3 4 ABSTRACT 40 41Coronavirus disease 2019 has caused more than 40,000 deaths 42 worldwide 1 . Approximately 14% of patients with COVID-19 experienced severe 43 disease and 5% were critically ill 2 . Studies have shown that dysregulation of the 44 COVID-19 patients' immune system may lead to inflammatory storm and cause severe 45 illness and even death 3,4 . Tocilizumab treatment targeting interleukin 6 receptor has 46shown inspiring clinical results of severe COVID-19 patients 5 . However, the immune 47 network with Tocilizumab treatment at single cell resolution has not been uncovered. 48Here, we profiled the single-cell transcriptomes of 13,289 peripheral blood 49 mononuclear cells isolated at three longitudinal stages from two severe COVID-19 50 patients treated with Tocilizumab. We identified a severe stage-specific monocyte 51 subpopulation and these cells centric immune cell interaction network connected by the 52 inflammatory cytokines and their receptors. The over-activated inflammatory immune 53 response was attenuated after Tocilizumab treatment, yet immune cells including 54 plasma B cells and CD8 + T cells still exhibited an intense humoral and cell-mediated 55 anti-virus immune response in recovered COVID-19 patients. These results provided 56 critical insights into the immunopathogenesis of severe COVID-19 and revealed 57 fundamentals of effectiveness in Tocilizumab treatment. 58 59 60 61Keywords ： Coronavirus disease 2019 (COVID-19); Severe acute respiratory 62 syndrome coronavirus 2 (SARS-CoV-2); Tocilizumab; Single-cell RNA sequencing 63(scRNA-seq); Inflammatory storm; Monocyte 64 65 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.

show abstract

mentioning

confidence: 99%

Single-cell analysis of severe COVID-19 patients reveals a monocyte-driven inflammatory storm attenuated by Tocilizumab

Guo

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Notably, gene expression changes persisted up to 1 month after infection. Similarly, studies of H7N9 infected patients showed transcriptional profile changes persisting up to 1 month with a transition from innate to adaptive immunity [86].…”

Section: Mechanisms Of Diseasementioning

confidence: 92%

“…However, direct links of events to viral infection are scarce in part due the event of interest may follow the infection by several weeks when the virus is no longer detectable by traditional testing. Several gene profiling studies have identified viral infection signatures that may persist up to 1-month post infection [79,86]. Thus, it might be possible to study patients with falls or cardiac events for evidence of recent viral infection using a host response viral signature.…”

Section: Associations With Respiratory Viral Infection With Non-respimentioning

confidence: 99%

Respiratory viral infections

Falsey

2019

Genomic and Precision Medicine

View full text Add to dashboard Cite

“…In April 2019 a fatal 66 case of highly pathogenic avian influenza (HPAI) H7N9 was reported in Inner Mongolia, China, 67 which was associated with severe pneumonia and respiratory failure [2]. Acute respiratory distress 68 syndrome, as observed in the recent human case of HPAI H7N9 infection, may be caused by multiple 69 factors, including higher viral load [3], altered viral tropism and spread in the lower/upper respiratory 70 tract [4], virally-induced host shutoff [5] or hijacking host function [6][7][8][9]. Mass spectrometry-based 71 serum proteome profiling has made it possible to conduct extracellular fluid proteins analysis and to 72 4 investigate important mediators of intercellular communication and host-viral interactions associated 73 with disease progression, coagulation, communication, inflammation and the immune response.…”

Section: Introduction 64mentioning

confidence: 99%

“…Our study identified key host responses that may 96 be related to the progression of H7N9 disease. 97 Supplementary Table 2; and clinical and 108 immunological features of some patients have been reported [3,14]. Ten healthy serum controls were 109 collected from our lab colleagues.…”

Section: Introduction 64mentioning

confidence: 99%

In-depth 15 H7N9 Human Serum Proteomics Profiling Study

Yang

Guan

Zhou³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

word count: 183 45 Text word count: 2981 46 47 3 Abstract 48Background. Human infection by avian influenza viruses is characterized by rapid development of 49 acute respiratory distress and severe pneumonia. However, the underlying host response leading to 50 this severe outcome is not well studied. 51Methods. We conducted mass spectrometry-based serum proteome profiling on 10 healthy controls 52 and 15 H7N9 infected cases with two time points and carried out statistical and biology functional 53 enrichment analysis. 54Results. In total, we identified 647 proteins, 273 proteins were only found in H7N9 infected cases 55 which might generate from cell leakage/death (apoptosis and/or necrosis) and identified 50 proteins 56 with statistically significant difference between healthy control and H7N9 infected cases from 168 57 qualified proteins. We also found that M1 and PB2 tightly associated with the host's HSPA8 (P11142, 58 p=0.0042) which plays an important role in the protein quality control system. 59Conclusions. H7N9 infection may increase cell programmed/unprogrammed cell death, and we 60 suggested that upregulated extracellular HSPA8 may suppress the H7N9 virion replication via 61 activation amyloid-beta binding network. 62

show abstract

Clinical Correlations of Transcriptional Profile in Patients Infected With Avian Influenza H7N9 Virus

Cited by 17 publications

References 38 publications

Single-cell analysis of severe COVID-19 patients reveals a monocyte-driven inflammatory storm attenuated by Tocilizumab

Single-cell analysis of severe COVID-19 patients reveals a monocyte-driven inflammatory storm attenuated by Tocilizumab

Respiratory viral infections

In-depth 15 H7N9 Human Serum Proteomics Profiling Study

Contact Info

Product

Resources

About