Identification of casein kinase Iα interacting protein partners

Abstract

Casein kinase IK K (CKIK K) belongs to a family of serine/threonine protein kinases involved in membrane trafficking, RNA processing, mitotic spindle formation and cell cycle progression. In this report, we identified several CKIK K interacting proteins including RCC1, high mobility group proteins 1 and 2 (HMG1, HMG2), Erf, centaurin-K K 1 , synaptotagmin IX and CPI-17 that were isolated from brain as CKIK K co-purifying proteins. Actin, importin-K K 1 , importin-L L, PP2Ac, centaurin-K K 1 , and HMG1 were identified by affinity chromatography using a peptide column comprising residues 214^233 of CKIK K. We have previously shown that centaurin-K K 1 represents a CKIK K partner both in vitro and in vivo. The nuclear protein regulator of chromosome condensation 1 (RCC1) is a guanosine nucleotide exchange factor for Ran which is involved in nuclear transport and mitotic spindle formation. Here we show that CKIK K and RCC1 interact in brain and in cultured cells. However, the interaction does not involve residues 217^233 of CKIK K which are proposed from X-ray structures to represent an anchoring site for CKI partners. Formation of the RCC1/CKIK K complex is consistent with the association of the kinase with mitotic spindles. In conclusion, we have identified a number of novel CKIK K protein partners and their relations to CKI are discussed. ß

Cloning of a centaurin-α1 like gene MjCent involved in WSSV infection from shrimp Marsupeneaus japonicus

“…Several studies have confirmed that this protein family is involved in the cell cytoskeleton [9][10][11] and regulates actin cytoskeleton organization in vivo as a GTPaseactivating protein (GAP) of ARF6 [12], a member of the ARF family of GTPases [13]. In addition, identifications of associations of centaurin-a1 with casein kinase Ia (CKIa) [14,15], Ran binding protein in microtubule-organizing center (RanBPM) [16], kinesin motor protein (KIF13B) [17,18] and protein kinase C (PKC) [19] further indicates the significant potential of centaurin-a1 in cellular cytoskeletal remodeling, vesicle trafficking and thus cellular innate immunity.…”

Physiological Role for Casein Kinase 1 in Glutamatergic Synaptic Transmission

“…In light of previous biochemical evidence for interactions between CK1 and the protein phosphatases PP1/PP2 A and between PP1/PP2 A and NMDA receptors (Agostinis et al, 1987;Marin et al, 1994;Westphal et al, 1999;Chan and Sucher, 2001;Dubois et al, 2002), we hypothesized that the protein phosphatases PP1 and/or PP2 A could be intermediary elements between CK1 and NMDA receptors. To examine whether inhibition of protein phosphatases might mediate the effect of CK1-7 on NMDA receptor activity, we applied the PP1/PP2 A inhibitor okadaic acid in either the perfusion solution or the intracellular solution and examined the effect of CK1-7 on NMDA-EPSCs.…”

RanBPM, a novel interaction partner of the brain‐specific protein p42IP4/centaurin α‐1

“…p42 IP4 can shuttle between cytosol, nucleus and plasma membrane, depending on the cell stimulation situation . Several interaction partners of p42 IP4 have been identified, such as casein kinase I (Dubois et al 2001(Dubois et al , 2002, nucleolin , protein kinase C , several ARFs (Thacker et al 2004;Venkateswarlu et al 2004) GAKIN (Venkateswarlu et al 2005;Horiguchi et al 2006), the peptidase nardilysin (Stricker et al 2006) or F-actin (Thacker et al 2004). It has been shown that p42 IP4 is a component of the neuronal phosphatidyl inositol 3-kinase cascade and is involved in dendritic differentiation/synaptic plasticity and/or transport of PtdIns(3,4,5)P 3 -containing vesicles along axons to regulate neuronal cell polarity (Horiguchi et al 2006;Moore et al 2007).…”