Identification of Candida spp. by phenotypic tests and PCR

Marinho, Sandra Aparecida; Teixeira, Alice Becker; Santos, Otávio Silveira; Cazanova, Ricardo Flores; Ferreira, Carlos Alexandre Sanchez; Cherubini, Karen; Oliveira, Sı́lvia Dias de

doi:10.1590/s1517-83822010000200004

Cited by 48 publications

(57 citation statements)

References 22 publications

(17 reference statements)

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“…Germ tube was considered as a lateral tube without septum and had no constriction at initiating site, which is a positive test for C. albicans (Marinho et al, 2010).…”

Section: Germ Tube Testmentioning

confidence: 99%

Molecular diagnosis of vaginal candidiasis by polymerase chain reaction (PCR) and random amplification polymorphism DNA (RAPD-PCR) in Babylon Province, Iraq

Zaidan¹,

Hadeel²

2014

Afr. J. Microbiol. Res.

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Most women suffer from severe vaginal candidiasis caused by Candida spp. Vaginal infections represent a second type of common disease. This infection occurs commonly in married and pregnant women and immunocompromised patients. This study aimed to survey, isolate and diagnose the vaginal Candida spp. using phenotypic and molecular assays. Candida isolates were recovered from patients attending main hospital in Babylon Province, Iraq from July 2011 to April 2012. Samples were subcultured on CHROMagar for preliminary identification of Candida isolates. Germ tube and chlamydospore formation were also performed to confirm identification. DNA of representative isolates was extracted for polymerase chain reaction (PCR) assays and analyzed using gel image patterns of bands for different isolates based on ultraviolet transilluminator band software. Results show that CHROMagar was a good tool for preliminary identification of Candida isolates. Molecular identification of PCR and random amplification polymorphism DNA (RAPD-PCR) assays were used to group the representative Candida isolates into different genetic patterns; phylogenetic tree was generated based on the information available in gel images. This study concludes that PCR and RAPD PCR assays confirmed more the diagnosis of Candida isolates than culture on CHROMagar medium and other phenotypic tests.

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“…Germ tube was considered as a lateral tube without septum and had no constriction at initiating site, which is a positive test for C. albicans (Marinho et al, 2010).…”

Section: Germ Tube Testmentioning

confidence: 99%

Molecular diagnosis of vaginal candidiasis by polymerase chain reaction (PCR) and random amplification polymorphism DNA (RAPD-PCR) in Babylon Province, Iraq

Zaidan¹,

Hadeel²

2014

Afr. J. Microbiol. Res.

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“…Biochemical characterization was carried out involving Isolation on HiCrome Candida Differential Agar (HiMedia Laboratories Pvt. Ltd., Mumbai), Germ tube test and urease production test (Marinho et al, 2010;Salviya et al, 2015). Identification of yeast other than Candida was performed by ethanol tolerance test (Guimarães et al, 2006).…”

Section: Biochemical Characterization Of Yeastmentioning

confidence: 99%

“…This indicates presence of non Candida albicans (NCA). To distinguish between Candida Spps HiChrome Candida Differential Agar was used (Marinho et al, 2010). Candida Differential Agar which, as per manufacturer's literature indicates presence of Candida krusei.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Isolation and Characterization of Bacteria and Yeast from Kombucha Tea

Mukadam¹,

Punjabi²,

Deshpande³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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“…Identification was carried out by performing Gram stain, germ tube test 24 (Fig. 2. showing germ tube test under 400x.…”

Section: Isolation and Identification Of Candida Sppmentioning

confidence: 99%

“…3. showing chlamydospore under 400x. ), growth at 45°C 24 and HiCrome candida differential agar medium 26 (Fig. 4.…”

Section: Isolation and Identification Of Candida Sppmentioning

confidence: 99%

Isolation and Identification of Candida Species in the Patients of Uti

Misra¹,

Jain²,

Singh³

et al. 2016

jemds

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Fungal infections of the urinary tract especially those caused by Candida species are becoming increasingly common due to prolonged antibiotic use, indwelling urinary catheters, and increase in the number of immunocompromised individuals. Symptoms of Candida pyelonephritis, cystitis, prostatitis, or epididymo-orchitis are little different from those of the same infections produced by other pathogens. Candiduria occurring in critically ill patients should initially be regarded as a marker for the possibility of invasive Candidiasis. The first step in further evaluation is to verify funguria by repeating the urinalysis and urine culture.

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Identification of Candida spp. by phenotypic tests and PCR

Cited by 48 publications

References 22 publications

Molecular diagnosis of vaginal candidiasis by polymerase chain reaction (PCR) and random amplification polymorphism DNA (RAPD-PCR) in Babylon Province, Iraq

Molecular diagnosis of vaginal candidiasis by polymerase chain reaction (PCR) and random amplification polymorphism DNA (RAPD-PCR) in Babylon Province, Iraq

Isolation and Characterization of Bacteria and Yeast from Kombucha Tea

Isolation and Identification of Candida Species in the Patients of Uti

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