Identification of Callery pear cultivars by peroxidase isozyme patterns

Santamour, Frank S.; Demuth, Polly

doi:10.1093/oxfordjournals.jhered.a109412

Cited by 30 publications

(19 citation statements)

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“…Examples are: callery pear (Pyrus calleryana Dene. ; Santamour & Demuth, 1980); Kentucky bluegrass (Poapratensis L.; Wu et al, 1984); and fine fescue (Festuca ovina L.; F. rubra L.; F. tenuifolia Sibth. ; Villamil et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Bermudagrass cultivar identification by use of isoenzyme electrophoretic patterns

1990

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Isoenzyme analysis has been demonstrated as an effective tool for definitive identification of plant cultivars, but it has not been applied to pasture bermudagrass (Cynodon spp.) cultivars in the USA. Polyac~lamide gel electrophoresis (PAGE) was used to study five isoenzyme systems in mitochondrial, microsomal, and soluble cell fractions of actively growing leaves, stems, and roots of seven vegetatively-propagated pasture bermudagrass (Cynodon spp.) cultivars used in the southern half of the USA. Peroxidase, esterase, and, with one exception, acid phosphatase successfully differentiated between the cultivars in all leaf and stem cell fractions. Fewer cultivar differences were found for amino-and endo-peptidases. Only peroxidase and acid phosphatase were resolved from root cell fractions; and only the microsomal fraction differentiated between all cultivars. Within plant parts, cultivars were distinguishable on the basis of peptidase banding in some cellular fractions, but not in others. Plant part and subcellular fraction-specific isoenzyme variations suggest the existence of multiple molecular forms of various enzymes within the same plant.

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Section: Introductionmentioning

confidence: 99%

Bermudagrass cultivar identification by use of isoenzyme electrophoretic patterns

1990

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“…Enzyme extraction and preparation is simple and the method can be used to rapidly determine an organism's genotype independent of judgement based on phenotypic information (9). Polyacrylamide and starch gel electrophoresis have been used to determine differences between cultivars and species of grape, pear and peach (I 0, II, 12,13). Viticulture is an ancient agricultural enterprises in Turkey and several cases of synonyms and homonyms have emerged among the different vine cultivars.…”

Section: Introductionmentioning

confidence: 99%

Isoenzymatic Polymorphism Differentiation of Turkish Grapevine Cultivars by Polyacrylamide Gel Electrophoresis

Türkben

Söylemezoğlu

Ergül

et al. 2002

Biotechnology & Biotechnological Equipment

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In this research isoenzymatic activity of Catechol oxidase (CO), Esterase (EST) and Peroxidase(PER) was studied in young leaves of 29 Turkish grape cultivars. Catechol oxidase, peroxidase and esterase isoenzyme banding patterns of 29 cultivars made it possible to distinguish between 29 Turkish grape cultivars. It was conculuded that all ofthe three enzyme systems provided the most useful/ data as it was possible to distinguish all 29 grape cultivars.

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“…Os trabalhos desenvolvidos por Santamour Junior & Demuth (1980), na cultura da pereira, com as formas isoenzimáticas da peroxidase permitiram identificar as cultivares do grupo caleriana (Pyrus calleryana Desc. ), já os trabalhos desenvolvidos por Daley et al (1987) permitiram diferenciar todas as cultivares de pereira (Pyrus communis L.), com exceção das mutantes de cor vermelha.…”

Section: Introductionunclassified

Polimorfismo enzimático nos tecidos de pereira

Fachinello

Musacchi

Zuccherelli

et al. 2000

Pesq. agropec. bras.

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O trabalho foi realizado com o objetivo de avaliar o polimorfismo enzimático em diferentes tecidos de oito cultivares de pereira Pyrus communis L. Os genótipos utilizados fazem parte da coleção de plantas disponíveis na Universidade de Estudos de Bolonha. Para as análises isoenzimáticas foram utilizadas gemas floríferas dormentes no inverno, casca de ramos de um ano obtida de plantas em pleno desenvolvimento, folhas obtidas no início da primavera e folhas de plantas mantidas in vitro. A corrida eletroforética foi realizada em gel de poliacrilamida a gradiente com (5% a 12,5%). Os resultados obtidos com os genótipos utilizados indicaram que o sistema enzimático beta-glucosidase (E.C.3.2.1.21) apresentou atividade apenas nas folhas das plantas in vitro, com uma banda na mesma posição para todas as cultivares, ao passo que os sistemas para as enzimas esterase (E.C.3.1.1.2) e peroxidase (E.C.1.11.1.7) apresentaram elevado polimorfismo. Nas gemas dormentes analisadas, o sistema peroxidase permitiu diferenciar todos os genótipos. As formas isoenzimáticas da esterase permitiram separar todos os genótipos independentemente dos tecidos utilizados.

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Identification of Callery pear cultivars by peroxidase isozyme patterns

Cited by 30 publications

References 0 publications

Bermudagrass cultivar identification by use of isoenzyme electrophoretic patterns

Bermudagrass cultivar identification by use of isoenzyme electrophoretic patterns

Isoenzymatic Polymorphism Differentiation of Turkish Grapevine Cultivars by Polyacrylamide Gel Electrophoresis

Polimorfismo enzimático nos tecidos de pereira

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