Bermudagrass cultivar identification by use of isoenzyme electrophoretic patterns

Abstract

Isoenzyme analysis has been demonstrated as an effective tool for definitive identification of plant cultivars, but it has not been applied to pasture bermudagrass (Cynodon spp.) cultivars in the USA. Polyac~lamide gel electrophoresis (PAGE) was used to study five isoenzyme systems in mitochondrial, microsomal, and soluble cell fractions of actively growing leaves, stems, and roots of seven vegetatively-propagated pasture bermudagrass (Cynodon spp.) cultivars used in the southern half of the USA. Peroxidase, esterase, and, with one exception, acid phosphatase successfully differentiated between the cultivars in all leaf and stem cell fractions. Fewer cultivar differences were found for amino-and endo-peptidases. Only peroxidase and acid phosphatase were resolved from root cell fractions; and only the microsomal fraction differentiated between all cultivars. Within plant parts, cultivars were distinguishable on the basis of peptidase banding in some cellular fractions, but not in others. Plant part and subcellular fraction-specific isoenzyme variations suggest the existence of multiple molecular forms of various enzymes within the same plant.

Response to glyphosate and electrophoretic variation ofCynodon dactylon (L) Pers populations

“…As a consequence, these enzymes were excluded from the analysis. Isozyme patterns of A‐PER, C‐PER, ACP and SKDH are in general agreement with those reported by Dabo et al 28 and Vermeulen et al 29. On the other hand, GPI phenotypes differ slightly from those reported by Vermeulen et al 29.…”

Identification of Vegetatively Propagated Turf Bermudagrass Cultivars Using Simple Sequence Repeat Markers

“…Vermeulen et al (1991) recognized the need to develop a reliable technique to facilitate the correct identifi cation of clonal turftype bermudagrass cultivars. Isoenzyme electrophoresis was used to identify clonal forage bermudagrass cultivars (Dabo et al, 1990). Six isozymes in starch gel electrophoresis identifi ed 16 of 21 turf bermudagrass genotypes, but the technique is limited because a large number of isoenzymes and their expression is infl uenced by the environment and the developmental stage (Vermeulen et al, 1991).…”

Genetic Variability of a Forage Bermudagrass Core Collection

“…Environmental effects reduce differentiation of true genetic diversity in low‐heritability traits. Differential levels of expressed gene products (Dabo et al, 1990; Gonnet, 1993; Grayer et al, 2004; Kaundun et al, 2000; Navarrete et al, 2006; Vermuelen et al, 1991) have also been used to measure diversity. However, gene expression may change under varying locations or harvest times (Brown et al, 2002; Hare, 2002; Lee et al, 2005).…”