Identification of Bufavirus-1 and Bufavirus-3 in Feces of Patients with Acute Diarrhea, China

Abstract

Bufavirus (BuV) is a newly discovered human parvovirus that has been detected in some countries. The current study was designed to understand the epidemic of BuV in China. Totally 1877 fecal specimens were collected from pediatric and adult patients with acute diarrhea in two large hospitals from 2010 to 2014. BuV was detected in 0.5% (9/1877) of the fecal samples by PCR and subsequent sequencing. The positive patients had a wide age range from 1 month through 60 years (median 24 years old) and 6 were male. A geographic specific pattern was obvious, with significantly higher frequency of BuV presented in Northern than in Southern China. Four BuV-1 and five BuV-3 were determined. Mixed-infections of BuV with sapovirus and novavirus were found in 2 cases, respectively. A temporal clustering was identified, with most positive detection focused in the cold weather. These findings have expanded the current knowledge on the geographic boundaries of BuV circulation.

Epidemiology of two human protoparvoviruses, bufavirus and tusavirus

“…Until now, human bufaviruses, mostly BuV1 or BuV3, has been detected by PCR in several geographical locations in Africa, Europe and Asia; but exclusively in diarrheal stools157891011. Studies have not included other sample types or other diagnostic means, yet the closely related BuV-like viruses have been found in spleens or sera of infected animals, indicating systemic infections1213.…”

“…The prevalence in patients has been low, 0.3% to 4%, and although in three publications stool samples from non-diarrheic patients or healthy subjects were shown to be BuV-DNA negative91011, the etiological role of the virus in diarrhoea or in other human diseases remains uncertain.…”

“…To date, bufavirus has been searched for exclusively in stool samples, and it has been detected in diarrheal stools of children and adults in Africa, Europe and Asia, including Burkina Faso, Tunisia, Bhutan, Finland, the Netherlands, Thailand, Turkey and China157891011. The prevalence in patients has been low, 0.3% to 4%, and although in three publications stool samples from non-diarrheic patients or healthy subjects were shown to be BuV-DNA negative91011, the etiological role of the virus in diarrhoea or in other human diseases remains uncertain.…”

Viruses of the Parvoviridae Family: Molecular Genetical Aspects of Reproduction and Medical Importance

“…Буфавирус человека был обнаружен в Туни-се, Буркина-Фасо, Бутане, Финляндии, Нидер-ландах, Китае [40,77,84].…”

“…Частота обнаружения ДНК BuPV варьировалась от 0,8 до 4% от числа обследован-ных лиц с острой диареей [40,84]. В Финляндии ДНК буфавируса обнаружена в 1,1% случаев га-строэнтерита (7/629) у лиц старше 21 года [77].…”