Identification of Brassinolide, Castasterone, Typhasterol, and Teasterone from the Pollen of<i>Lilium elegans</i>

Suzuki, Hiroyuki; Fujioka, Shozo; Yokota, Takao; Murofushi, Noboru; Sakurai, Akira

doi:10.1271/bbb.58.2075

Cited by 13 publications

(3 citation statements)

References 6 publications

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“…x 20.0 em, Kieselgel 60, 230-400mesh, Merck). Elution was done stepwise with CHCI 3 (lOOml), 2% (lOOml), 4,6,8,10,12,15,20,30, and 100% MeOH in CHCI 3 (each 50 ml). Strongly active eluates with 4 and 6% MeOH in CHCl 3 were combined into fraction P-I1-a (38mg).…”

Section: Methodsmentioning

confidence: 99%

Free and Conjugated Brassinosteroids in the Pollen and Anthers ofErythronium japonicumDecne.

Yasuta

Terahata

Nakayama

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Free and Conjugated Brassinosteroids in the Pollen and Anthers ofErythronium japonicumDecne.

Yasuta

Terahata

Nakayama

et al. 1995

Bioscience, Biotechnology, and Biochemistry

Self Cite

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“…Concordantly, different types of BRs showed tissue-and developmental-specific profiles in grapevines. TY is an intermediary in the early C-6 oxidation route and has biological activity itself [66]. The accumulation of TY and CS could indicate that the early C-6 oxidation biosynthesis pathway is active in grapevines.…”

Section: Endogenous Levels Of Brassinosteroids In Grapevinesmentioning

confidence: 99%

Characterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines

Parada

Oklešťková

Arce‐Johnson

2022

IJMS

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Agronomic breeding practices for grapevines (Vitis vinifera L.) include the application of growth regulators in the field. Brassinosteroids (BRs) are a family of sterol-derived plant hormones that regulate several physiological processes and responses to biotic and abiotic stress. In grapevine berries, the production of biologically active BRs, castasterone and 6-deoxocastasterone, has been reported. In this work, key BR genes were identified, and their expression profiles were determined in grapevine. Bioinformatic homology analyses of the Arabidopsis genome found 14 genes associated with biosynthetic, perception and signaling pathways, suggesting a partial conservation of these pathways between the two species. The tissue- and development-specific expression profiles of these genes were determined by qRT-PCR in nine different grapevine tissues. Using UHPLC-MS/MS, 10 different BR compounds were pinpointed and quantified in 20 different tissues, each presenting specific accumulation patterns. Although, in general, the expression profile of the biosynthesis pathway genes of BRs did not directly correlate with the accumulation of metabolites, this could reflect the complexity of the BR biosynthesis pathway and its regulation. The development of this work thus generates a contribution to our knowledge about the presence, and diversity of BRs in grapevines.

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“…Interestingly, monocots have only one copy of a BR6OX gene while dicots have two or more copies, providing additional evidence that CS might be the final product of BR biosynthesis in many monocots including rice [32]. However, it must be mentioned that BL has been detected in a few monocots, for instance in Lilium elegans [65].…”

Section: Introductionmentioning

confidence: 99%

Inhibitors of Brassinosteroid Biosynthesis and Signal Transduction

et al. 2019

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Chemical inhibitors are invaluable tools for investigating protein function in reverse genetic approaches. Their application bears many advantages over mutant generation and characterization. Inhibitors can overcome functional redundancy, their application is not limited to species for which tools of molecular genetics are available and they can be applied to specific tissues or developmental stages, making them highly convenient for addressing biological questions. The use of inhibitors has helped to elucidate hormone biosynthesis and signaling pathways and here we review compounds that were developed for the plant hormones brassinosteroids (BRs). BRs are steroids that have strong growth-promoting capacities, are crucial for all stages of plant development and participate in adaptive growth processes and stress response reactions. In the last two decades, impressive progress has been made in BR inhibitor development and application, which has been instrumental for studying BR modes of activity and identifying and characterizing key players. Both, inhibitors that target biosynthesis, such as brassinazole, and inhibitors that target signaling, such as bikinin, exist and in a comprehensive overview we summarize knowledge and methodology that enabled their design and key findings of their use. In addition, the potential of BR inhibitors for commercial application in plant production is discussed.

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Identification of Brassinolide, Castasterone, Typhasterol, and Teasterone from the Pollen ofLilium elegans

Cited by 13 publications

References 6 publications

Free and Conjugated Brassinosteroids in the Pollen and Anthers ofErythronium japonicumDecne.

Free and Conjugated Brassinosteroids in the Pollen and Anthers ofErythronium japonicumDecne.

Characterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines

Inhibitors of Brassinosteroid Biosynthesis and Signal Transduction

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