Identification of bovine and porcine rotavirus G types by PCR

Gouvêa, Vera; Santos, Norma; Timenetsky, María do Carmo Sampaio Tavares

doi:10.1128/jcm.32.5.1338-1340.1994

Cited by 239 publications

(103 citation statements)

References 23 publications

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“…2.1 (TaKaRa Bio Inc., Shiga, Japan). RNA extracts were used for detection of BCoV and BCoV-like viruses (Erles et al, 2003), toroviruses (Hoet et al, 2002), rotaviruses (Gouvea et al, 1994), caliciviruses (Jiang et al, 1999), bovine viral diarrhea virus (BVDV) (Sullivan and Akkina, 1995) and bovine respiratory syncytial virus (Valarcher et al, 1999). RT-PCR assays were performed using SuperScript TM One-…”

Section: Screening For Viral Pathogensmentioning

confidence: 99%

Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves

Decaro¹,

Cirone²,

Mari³

et al. 2010

Veterinary Microbiology

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Recently, a coronavirus strain (179/07-11) was isolated from water buffalo (Bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (BCoV) was referred to as bubaline coronavirus (BuCoV). Here, we report the characterisation of four BuCoVs strains identified in the faeces or intestinal contents of water buffalo calves with acute gastroenteritis. Single BuCoV infections were detected in all but one cases from which two clostridia species were also isolated. Sequence and phylogenetic analyses of the 5' end of the spike-protein gene showed that three BuCoVs were closely related to the prototype strain 179/07-11, whereas the fourth isolate (339/08-C) displayed a higher genetic identity to recent BCoV reference strains. Three strains adapted to the in vitro grow on human rectal tumour cells were also evaluated for their ability to replicate in a bovine cell line (Madin Darby bovine kidney) and to cause haemagglutination of chicken erythrocytes and all displayed biological properties similar to those already described for the prototype BuCoV. The present report shows that albeit genetically heterogeneous, the different BuCoV strains possess a common biological pattern which is different from most BCoV and BCoV-like isolates.

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Section: Screening For Viral Pathogensmentioning

confidence: 99%

Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves

Decaro¹,

Cirone²,

Mari³

et al. 2010

Veterinary Microbiology

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“…RNA and DNA extracts from nasal and ocular swabs were tested by nested PCR for bovine respiratory syncytial virus (BRSV) (Valarcher et al, 1999) and by PCR for bovine herpesviruses types 1 (BoHV-1) (Vilcek, 1993) and 4 (BoHV-4) (Boerner et al, 1999). RNA extracts from rectal swabs were used for detection of bovine torovirus , as well as rotaviruses (Gouvea et al, 1994) and caliciviruses (Jiang et al, 1999), whereas RT-PCR for bovine viral diarrhoea virus (BVDV) (Sullivan and Akkina, 1995) was carried out on RNAs purified from blood, nasal and faecal samples. RT-PCR and PCR assays were performed using SuperScript TM One-Step RT-PCR for Long Templates (Life Technologies) and LA PCR Kit Ver.…”

Section: (Rt)-pcr Assay For Screening For Other Bovine Viral Pathogensmentioning

confidence: 99%

Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season

Decaro¹,

Mari²,

Desario³

et al. 2008

Veterinary Microbiology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t

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“…Molecular methods were used for detection of other viral pathogens of dogs, including mammalian reoviruses (MRV) [19,20], rotaviruses [21], caliciviruses [22,23], canine adenoviruses [24], canine distemper virus (CDV) [25], canine herpesvirus [26] and canine coronavirus (CCoV) [27,28]. Specimens that tested positive for CPV vaccine strains and negative for viral pathogens were examined for bacterial and parasitic pathogens by standardised methods.…”

Section: Screening Of Faecal Samples For Other Canine Pathogensmentioning

confidence: 99%

Occurrence of severe gastroenteritis in pups after canine parvovirus vaccine administration: A clinical and laboratory diagnostic dilemma

Decaro¹,

Desario²,

Elia³

et al. 2007

Vaccine

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A total of 29 faecal samples collected from dogs with diarrhoea following canine parvovirus (CPV) vaccination were tested by minor groove binder (MGB) probe assays for discrimination between CPV vaccine and field strains and by diagnostic tests for detection of other canine pathogens. Fifteen samples tested positive only for CPV field strains; however, both vaccine and field strains were detected in three samples. Eleven samples were found to contain only the vaccine strain, although eight of them tested positive for other pathogens of dogs. Only three samples were found to contain the vaccine strain without evidence of canine pathogens. The present study confirms that most cases of parvovirus-like disease occurring shortly after vaccination are related to infection with field strains of canine parvovirus type 2 (CPV-2) rather than to reversion to virulence of the modified live virus contained in the vaccine.

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Identification of bovine and porcine rotavirus G types by PCR

Cited by 239 publications

References 23 publications

Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves

Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves

Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season

Occurrence of severe gastroenteritis in pups after canine parvovirus vaccine administration: A clinical and laboratory diagnostic dilemma

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