Occurrence of severe gastroenteritis in pups after canine parvovirus vaccine administration: A clinical and laboratory diagnostic dilemma

Decaro, Nicola; Desario, Costantina; Elia, Gabriella; Campolo, Marco; Lorusso, Alessio; Mari, Viviana; Martella, Vito; Buonavoglia, Canio

doi:10.1016/j.vaccine.2006.10.020

Cited by 94 publications

(87 citation statements)

References 30 publications

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“…The genetic identity between the 'field' strain 490/07 and the vaccine virus Felocell suggests a possible isolation of the vaccine strain from a cat recently vaccinated. In fact, it is well known that modified-live parvoviruses contained in the vaccines are able to replicate in the intestinal mucosa of vaccinated animals and to be shed with the dog or cat faeces (Decaro et al, 2007;Patterson et al, 2007). In our case, the anamnesis of the cat shedding a Felocell-like FPLV strain was not known, thus preventing the unambiguous identification of strain 490/07 as vaccine virus.…”

Section: Discussionmentioning

confidence: 80%

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Decaro¹,

Desario²,

Miccolupo

et al. 2008

Journal of General Virology

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Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n534) and UK (n55) from cats with feline panleukopenia were characterized at the molecular level. All viruses were proven to be true feline panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene, it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide identity of 99.1-100 and 99.4-99.8 % among Italian and British strains, respectively, whereas the similarities between all the sequences analysed were 98.6-100 %. Eighty-eight variable positions were detected in the VP2 gene of the field and reference FPLV strains, most of which were singletons. Synonymous substitutions (n557) predominated over non-synonymous substitutions (n531), and the ratio between synonymous and non-synonymous substitutions (dN/dS) was 0.10, thus confirming that evolution of FPLV is driven by random genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.

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Section: Discussionmentioning

confidence: 80%

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Decaro¹,

Desario²,

Miccolupo

et al. 2008

Journal of General Virology

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“…The amino acid residue 440 is important because it is located at the top of the 3-fold spike (GH loop) of the VP2 protein on the surface of the capsid, the main antigenic site of the virus [49,50]. This residue was undergoing positive selection for the past few years and had evolved independently in different populations, which explained its world-wide mutation in unrelated CPV-2 populations [51]. Similar codon change at amino acid position 440 was also reported in CPVs isolated in different countries [28,44,52].…”

Section: Discussionmentioning

confidence: 99%

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala¹,

Mukhopadhyay²,

Sivaprakasam³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.

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“…The reports of gastroenteritis subsequent to vaccination are related to infection with CPV field strains shortly before or after the vaccine administration [23]. It has previously been demonstrated that a type 2 vaccine is able to provide protection against type 2a and 2b field isolates [27].…”

Section: Immunizationmentioning

confidence: 99%