Identification of bla KPC-2 on different plasmids of three Morganella morganii isolates

Shi, Dongfang; Wang, W.-P.; Kuai, Shougang; Shao, Haifeng; Huang, Mei‐Rong

doi:10.1007/s10096-011-1377-9

Cited by 12 publications

(9 citation statements)

References 21 publications

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“…Genes from M. morganii that encode cefotaxime-hydrolysing β-lactamases have also been reported with increasing frequency [42]. Plasmid-borne drug resistance factors have also increased the virulence of M. morganii [26,27]. Gene products that confer multidrug resistance, including metallo-β-lactamases and efflux pumps for tetracycline, tellurite, bicyclomycin, and kasugamycin, have been commonly reported for clinical isolates of M. morganii .…”

Section: Discussionmentioning

confidence: 99%

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Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

et al. 2012

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BackgroundThe opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria.ResultsThe 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes.ConclusionsThis is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.

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Section: Discussionmentioning

confidence: 99%

“…The drug resistance of M . morganii was introduced via extra genetic elements [26,27] and/or mobile elements [23,24]. The resistant strains that carry bla CTX-M gene are capable of producing β-lactamases [28], which can break down the extended spectrum β-lactam drugs [29].…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

et al. 2012

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“…Rifampicin-resistant Escherichia coli EC600 (LacZ − Nal r Rif r ) was used as the recipient strain. A conjugation experiment was carried out in mixed-broth cultures as described previously (Shi et al , 2012). Escherichia coli transconjugants were selected on Mueller–Hinton agar plates containing 256 µg rifampicin ml −1 (Sigma) plus 1 µg imipenem ml −1 .…”

Section: Methodsmentioning

confidence: 99%

“…Soon afterwards, these plasmid-mediated carbapenemases were identified in multiple genera and species of the Enterobacteriaceae and non-fermenters. The geographical distribution of bla KPC -producing isolates has spread not only within the USA (Bradford et al , 2004; Bratu et al , 2005), but also in Israel (Navon-Venezia et al , 2006), France (Naas et al , 2005), Greece (Cuzon et al , 2008), China (Shi et al , 2012), Argentina and Brazil (Monteiro et al , 2009).…”

Section: Introductionmentioning

confidence: 99%

KPC-2 carbapenemase and DHA-1 AmpC determinants carried on the same plasmid in Enterobacter aerogenes

Kuai

Shao

Huang

et al. 2014

Journal of Medical Microbiology

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This study was conducted to analyse the presence of a plasmid-mediated carbapenem resistance mechanism in a clinical Enterobacter aerogenes isolate from a patient from Jiangsu province, People's Republic of China. PCR and sequencing confirmed that the isolate harboured Klebsiella pneumoniae carbapenemase (KPC)-2, DHA-1 and TEM-1 b-lactamase genes. Both the KPC-2 and DHA-1 genes were transferred to Escherichia coli C600 by transconjugation, and Southern blotting confirmed that these two genes were located on the same plasmid, which was of approximately 56 kb in size. The Enterobacter aerogenes isolate was resistant to carbapenems and other tested antimicrobial agents. The Escherichia coli transconjugant showed reduced susceptibility but not resistance to carbapenems and other b-lactams, indicating the presence of another, possibly permeability-related, resistance mechanism in the clinical isolate.

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“…Si bien K. pneumoniae es la principal especie productora de KPC, también se ha determinado su presencia en otras especies de la familia Enterobacteriaceae, como K. oxytoca, Salmonella enterica, E. coli, Proteus spp., Citrobacter freundii, Enterobacter spp., Raoultella ornithinolytica, Morganella morganii, Pantoea spp., Providencia spp. y S. marcescens [23][24][25][26][27][28][29] . Adicionalmente, se ha determinado la presencia de esta enzima en bacilos gramnegativos no fermentadores como A. baumannii, P. aeruginosa y Flavobacterium odoratum 17,[30][31] .…”

Section: Kpc (Klebsiella Pneumoniae Carbapenemasa)unclassified

KPC: Klebsiella pneumoniae carbapenemasa, principal carbapenemasa en enterobacterias

Vera-Leiva¹,

Barría-Loaiza²,

Carrasco-Anabalón³

et al. 2017

Rev. chil. infectol.

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The dissemination of carbapenemase-producing Enterobacteriaceae is currently considered a serious clinical problem due to the failure in the treatment of infections produced by them. Among the carbapenemases, the enzyme KPC has spread worldwide and has been identified in the main enterobacterial species related with healthcareassociated infections, although Klebsiella pneumoniae is the predominant specie. The blaKPC gene is transported, mainly by the transposon Tn4401, detected in various enterobacterial species of different sequence types (ST) and geographical origin. In addition, new genetic platforms that are distinguished, from Tn4401 because of insertions or deletions of other genes have been described. Plasmids containing the blaKPC gene can be conjugative and mobilizable non-conjugative plasmids, and can carry other genetic determinants of resistance. The KPC-producing strains may have different levels of resistance to carbapenems, due to the involvement of additional mechanisms such as different expression levels of porins and efflux pumps associated with the production of extended spectrum β-lactamases and/or AmpC. However, the carbapenemases, with KPC as the most common enzyme, provide higher levels of resistance.

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Identification of bla KPC-2 on different plasmids of three Morganella morganii isolates

Cited by 12 publications

References 21 publications

Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

KPC-2 carbapenemase and DHA-1 AmpC determinants carried on the same plasmid in Enterobacter aerogenes

KPC: Klebsiella pneumoniae carbapenemasa, principal carbapenemasa en enterobacterias

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