Identification of Biomarkers of Autophagy-Related Genes Between Early and Advanced Carotid Atherosclerosis

Zhang, Yuanyuan; Zhang, He

doi:10.2147/ijgm.s350232

Cited by 4 publications

(2 citation statements)

References 54 publications

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“…ITGB1 was also reported to be associated with the occurrence of coronary heart disease and was downregulated in advanced stage of atherosclerosis. 31 The negative regulation of ITGB1 was observed by miR-124-3p, and the regulatory effect of the PCA3/ miR-124-3p axis was also demonstrated in VSMCs in the present study. Therefore, ITGB1 was speculated to mediate the regulatory effect of the PCA3/miR-124-3p axis on VSMCs.…”

Section: Discussionsupporting

confidence: 71%

lncRNA PCA3 Suppressed Carotid Artery Stenosis and Vascular Smooth Muscle Cell Function via Negatively Modulating the miR-124-3p/ITGB1 Axis

Qiang

2023

Clin Appl Thromb Hemost

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Background & objectives Due to the hidden pathogen, carotid artery stenosis (CAS) always occurred at an advanced stage leading to serious sequelae and even deaths. The significance of long noncoding RNA (lncRNA) prostate cancer antigen 3 (PCA3) in CAS incidence and progression were evaluated aiming to explore a potential target for its therapy. Materials and methods Serum samples were collected from 83 asymptomatic CAS patients and 52 healthy individuals and PCA3 was compared using polymerase chain reaction (PCR). The PCA3 levels were compared between stable and unstable plaque in CAS patients. The effect of PCA3 on vascular smooth muscle cells (VSMCs) proliferation and motility was assessed by CCK8 and transwell assay. Results PCA3 was downregulated in CAS patients and their unstable plaque tissues compared with healthy individuals and stable plaque, respectively. Reduced PCA3 could discriminate CAS patients with relatively high sensitivity and specificity and were associated with higher total cholesterol level and stenosis degree, unstable plaque, and complications. PCA3 downregulation predicted the adverse outcomes of CAS patients. In VSMCs, overexpressing PCA3 significantly suppressed cell proliferation, migration, and invasion, which was alleviated by miR-124-3p/ITGB1 axis. Conclusion PCA3 served as a biomarker of CAS and regulates the function of VSMCs through sponging miR-124-3p/ITGB1 and indirectly influence the stability of plaque.

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Section: Discussionsupporting

confidence: 71%

lncRNA PCA3 Suppressed Carotid Artery Stenosis and Vascular Smooth Muscle Cell Function via Negatively Modulating the miR-124-3p/ITGB1 Axis

Qiang

2023

Clin Appl Thromb Hemost

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“…CTSB is a member of the C1 family of peptidases, and overexpression of this protein has been linked to various tumors, including esophageal adenocarcinoma [38] . Studies have shown that CTSB is upregulated in atherosclerotic plaques of rabbit carotid arteries following an atherogenic diet and balloon injury [39] . Furthermore, it has been suggested that the expression levels of CTSB, which is associated with autophagy, are upregulated in CHD tissues [40] .…”

Section: Discussionmentioning

confidence: 99%

Identifying ferroptosis-related genes associated with coronary heart disease based on bioinformatics analysis

Chai

et al. 2023

Preprint

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Background:Coronary heart disease (CHD) is a prevalent type of organ disease, caused by atherosclerosis, and is the leading cause of global human mortality. Ferroptosis, a recently discovered form of programmed cell death, is known to be involved in the pathogenesis of various diseases. In this study, we aimed to investigate the differential expression of ferroptosis-related genes (FRGs) and their potential relationship with CHD. Methods: The CHD datasets were obtained from the Gene Expression Omnibus (GEO) database and microarray data was normalized and analyzed for differential expression genes (DEGs) using R software. FRGs were retrieved from the FerrDb and GeneCards databases, and their intersection with DEGs was determined using Venn diagrams. The differential expression FRGs were subjected to further analyses, including GO, KEGG, GSEA, transcription factor analysis, and PPI network analysis. Immune infiltration analysis was performed on both control and CHD groups, and diagnostic biomarkers for CHD were identified by constructing ROC curves. Finally, the expression of the diagnostic biomarkers at the gene level in mice was validated using qRT-PCR assay. Results: Our study identified 25 DEGs associated with ferroptosis, which were enriched in various biological processes, including response to extracellular stimulus, regulation of protein ubiquitination, DNA-templated, and autophagy of mitochondrion. The KEGG pathway and GSEA indicated that these DEGs were mainly involved in cytokine-cytokine receptor interaction and the NF-kappa B signaling pathway. The PPI network analysis identified 6 key genes in the network. The correlation analysis between the expression of these key genes and immune cells showed a significant relationship. Diagnostic biomarkers for CHD, including ICTSB, GABARAPL1, and VDR, were identified using ROC analysis. The expression trends of these biomarkers were further validated using qRT-PCR, which confirmed the consistency with the bioinformatics analysis. Conclusions: Our study identified CTSB, GABARAPL1, and VDR as potential diagnostic biomarkers for CHD. In the future, in-vivo studies are necessary to validate our findings and confirm their clinical utility.

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The Pathogenetic Significance of miR-143 in Atherosclerosis Development

Lopatin

Vulf

Bograya

et al. 2023

Bioinformatics and Biomedical Engineering

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Identification of Biomarkers of Autophagy-Related Genes Between Early and Advanced Carotid Atherosclerosis

Cited by 4 publications

References 54 publications

lncRNA PCA3 Suppressed Carotid Artery Stenosis and Vascular Smooth Muscle Cell Function via Negatively Modulating the miR-124-3p/ITGB1 Axis

lncRNA PCA3 Suppressed Carotid Artery Stenosis and Vascular Smooth Muscle Cell Function via Negatively Modulating the miR-124-3p/ITGB1 Axis

Identifying ferroptosis-related genes associated with coronary heart disease based on bioinformatics analysis

The Pathogenetic Significance of miR-143 in Atherosclerosis Development

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