Identification of Bio-oil Compound Utilizing Yeasts Through Phenotypic Microarray Screening

Abstract

Biomass pyrolysis bio-oil contains a plethora of carbon sources with the potential to be utilized by microorganisms and converted into high value products. However, the majority of these compounds are either highly toxic to microorganisms or are not directly utilizable. Hence research is required to develop methods of separating microbe friendly compounds from inhibitory ones, and to also identify novel microorganisms that can utilise the principal carbon sources in pyrolysis bio-oil. This study employed a phenotypic microarray (PM) technique that measured yeast metabolic output to screen for and shortlist yeast strains able to metabolize various bio-oil carbon sources, with a focus on the anhydrosugar levoglucosan. Four strains of yeast (two Pichia spp. and two Kluyveromyces spp.) were shortlisted due to their high metabolic output (between 79.7 and 113.7 redox signal intensity) on levoglucosan from the PM assay. Under anaerobic fermentation conditions the strains were able to uptake levoglucosan (between 79 and 100% uptake efficiency) but not produce bioethanol; yet trace amounts of acetic acid were generated. This study demonstrated the application of applying the PM technique to screen for novel yeast strains with abilities to metabolize compounds present in pyrolysis bio-oil that could lead to the identification of novel levoglucosan utilization pathways.

Identification and Growth Characterization of a Novel Strain of Saccharomyces boulardii Isolated From Soya Paste

“…For the species-level identification, the BIOLOG TM identification system was applied (BIOLOG TM , USA) based on the utilization of carbon sources and chemical sensitivity assays in yeast identification microplate (YT) test panels (11,33). All the chemicals, reagents, and growth medium were supplied by (BIOLOG TM , USA).…”

“…Repeating Pipettor was filled by drawing up the cell suspension from the reservoir and all 96 wells were inoculated with 100 µl of the suspension. The microplate was then covered with its lid and incubated at 30 • C for 24-48 h. After incubation, the microplate was placed into the Micro Station Reader for analysis to obtain ID result and the result was given through comparison with the database using the software program MicroLog 4.20.05 (BIOLOG TM , USA) (33,34). The scope of the 96 assay reactions, coupled with sophisticated interpretation software, delivers a high level of accuracy that is comparable to molecular methods.…”

“…The strain was identified with BIOLOG TM microarray identification system up to the species level ( Table 2). For further confirmation, these isolates were examined for three replications (11,33,34).…”

The Prospects of Lactobacillus oris as a Potential Probiotic With Cholesterol-Reducing Property From Mother's Milk

“…BioLog TM (BIOLOG™, USA) is an advanced microbial identification system that can swiftly identify species of aerobic and anaerobic bacteria, yeasts, and fungi. The latest generation redox chemistry of the BioLog TM enables testing and identification of aerobic gram-negative and gram-positive bacteria in the same test panel ( 20 ). For the species-level identification of predicted Lactobacillus sp.…”

Solvent-free manufacture of methacrylate polymers from biomass pyrolysis products

“…The area percentage method was used for the quantification of the compounds present in the pyrolysis liquid. 5,26 Identification of individual compounds was performed using the NIST library. All peaks below 1% intensity or below 80% match probability were excluded from analysis.…”