Identification of bacteriocinogenic Lactococcus isolates from raw milk and cheese capable of producing nisin A and nisin Z

Perin, Luana Martins; Moraes, Paula Mendonça; Viçosa, Gabriela Nogueira; Júnior, Abelardo Silva; Nero, Luís Augusto

doi:10.1016/j.idairyj.2011.12.010

Cited by 27 publications

(30 citation statements)

References 40 publications

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“…In previous studies from our group (Ortolani et al, 2010;Perin et al, 2012), L. lactis subsp. lactis Lc08 was isolated from raw milk and characterised molecularly and phenotypically as a bacteriocinogenic strain capable of producing nisin Z and presenting a broad inhibitory activity against several foodborne pathogens.…”

mentioning

confidence: 69%

“…lactis based on 16s rDNA sequencing and species-specific PCR (Perin et al, 2012). Lc08 was previously characterised as a bacteriocinogenic strain capable of producing nisin Z. Lc25 was identified as a non-bacteriocinogenic strain and was used in the present study as a negative control for bacteriocin production.…”

Section: Methodsmentioning

confidence: 99%

“…Lc08 was previously characterised as a bacteriocinogenic strain capable of producing nisin Z. Lc25 was identified as a non-bacteriocinogenic strain and was used in the present study as a negative control for bacteriocin production. Based on the antimicrobial activity of Lc08 against several foodborne pathogens and spoilage microorganisms (Perin et al, 2012), L. monocytogenes ATCC 7644 was selected as the target. LAB strains were stored in de Mann, Rogosa and Sharpe broth (MRS, Oxoid Ltd., Basingstoke, England), and L. monocytogenes was stored in Trypticase Soy Broth supplemented with 0.6% (w/v) Yeast Extract (TSA-YE, Oxoid).…”

Section: Methodsmentioning

confidence: 99%

“…Nisin (called nisin A) was the first wellcharacterised bacteriocin, and structural variants of this protein include nisin Q, U and Z (Piper et al, 2011;Perin et al, 2012). Nisin Z usually exhibits a higher inhibitory activity than nisin A, most likely due to its higher diffusion capacity in the solid culture media employed in tests to detect antagonistic activity (Piper et al, 2011).…”

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confidence: 99%

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Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

Perin

Miranda

Camargo

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65°C for 30min, 75°C for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at autoclave conditions result in loss of their antimicrobial activity. Lc08 presented antimicrobial activity against L. monocytogenes in the milk system after 12h at 25°C. No effect was found at 7°C. The results show the application viability of the Lc08 in food systems as a biopreservative against L. monocytogenes.

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mentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

Perin

Miranda

Camargo

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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“…lactis (15,16,17,40,41,42,43). To the best of our knowledge, molecular identification studies specifically on wild nisin-producing (Nis ϩ ) L. lactis subsp.…”

mentioning

confidence: 99%

Characterization of a Wild, Novel Nisin A-Producing Lactococcus Strain with an L. lactis subsp. cremoris Genotype and an L. lactis subsp. lactis Phenotype, Isolated from Greek Raw Milk

Parapouli

Delbès-Paus²,

Kakouri³

et al. 2013

Appl Environ Microbiol

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. In this study, the actual subspecies identity of M78 and M104 isolates was elucidated, using 16S rRNA and acmA (encoding lactococcal N-acetylmuramidase) gene and histidine biosynthesis operon polymorphisms and 16S rRNA and ldh (encoding lactate dehydrogenase) gene phylogenies. Except the acmA gene analysis, molecular tools revealed that isolates M78 and M104 clustered with strains of the cremoris genotype, including the LMG 6897 T strain, while they were distant from strains of the lactis genotype, including the LMG 6890 T strain. The two wild isolates had identical repetitive sequence-based PCR (rep-PCR), randomly amplified polymorphic DNA (RAPD), plasmid, and whole-cell protein profiles and shared high 16S rRNA (99.9%) and ldh (100%) gene sequence homologies. In contrast, they exhibited identical sugar fermentation and enzymatic patterns which were similar to those of the subspecies lactis LMG 6890 T strain. To our knowledge, this is the first complete identification report on a wild L. lactis subsp. cremoris genotype of the lactis phenotype which is capable of nisin A production and, thus, has strong potential for use as a novel dairy starter and/or protective culture.

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Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources

Dong

et al. 2020

Listeria Monocytogenes

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Identification of bacteriocinogenic Lactococcus isolates from raw milk and cheese capable of producing nisin A and nisin Z

Cited by 27 publications

References 40 publications

Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

Characterization of a Wild, Novel Nisin A-Producing Lactococcus Strain with an L. lactis subsp. cremoris Genotype and an L. lactis subsp. lactis Phenotype, Isolated from Greek Raw Milk

Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources

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