Identification of bacterial cultures from archaeological wood using molecular biological techniques

Helms, A.C.; Martiny, Adam C.; Hofman-Bang, H. Jacob Peider; Ahring, Birgitte Kiær; Kilstrup, Mogens

doi:10.1016/j.ibiod.2003.10.003

Cited by 33 publications

(2 citation statements)

References 25 publications

(28 reference statements)

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“…However, waterlogged wooden remains are subject to shrinkage damage, and some even lose up to 80% of their original volume when exposed to air [37]. When such objects are allowed to dry, they may collapse [38]. This is the reason why lacquer plates excavated from the Nanhai No.…”

Section: Discussionmentioning

confidence: 99%

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Bacterial and Biodeterioration Analysis of the Waterlogged Wooden Lacquer Plates from the Nanhai No. 1 Shipwreck

et al. 2019

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To protect the lacquer plates from the Nanhai No. 1 shipwreck from being corroded by microorganisms, a series of studies were conducted on the four water-stored samples. The water samples were collected from the vessel where the lacquerware was stored in June and December 2017. In our study, high-throughput sequencing was conducted to reveal predominant bacterial communities. Then, three different media were used to isolate the dominant bacteria, and the 16S rRNA genes were sequenced. Next, we tested the degradation activity of lignin and cellulose by the isolated bacterial strains. After being cultured on a medium containing carboxylmethylcellulose (CMC), almost all the isolated strains (except Microbacterium sp. NK-NH4, Ochrobactrum sp. NK-NH9, and Bacillus megaterium NK-NH10) showed the capacity for cellulose degradation. In addition, the lignin peroxidase (Lip) and laccase activity of the strains were shown by culturing the strains on one medium with azure and on another medium with Remazol brilliant blue. The results indicated that the Lip activity of all the strains was low, whereas the laccase activity of Microbacterium sp. NK-NH4, Bacillus tequilensis NK-NH5, Bacillus subtilis NK-NH6, Bacillus megaterium NK-NH10, and Bacillus velezensis NK-NH11 was relatively high. Finally, we tested the bacteriostatic efficacy of four biocides—Preventol® D7, BIT 20N, P91, and Euxyl® K100. We found that most strains were sensitive to D7 and 20N, while K100 had almost no impact.

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Section: Discussionmentioning

confidence: 99%

“…While in storage, the water should be replaced at regular intervals. Furthermore, cold, dark and near-anaerobic conditions result in slower degradation [38]. Since the lacquer plates from the Nanhai No.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and Biodeterioration Analysis of the Waterlogged Wooden Lacquer Plates from the Nanhai No. 1 Shipwreck

et al. 2019

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Archaeological Wood

2017

Chemicals and Methods for Conservation and Restoration

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Physiology of biodeterioration on canvas paintings

Poyatos

Morales

Nicholson

et al. 2017

Journal Cellular Physiology

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The study of the physiological action of microorganisms in artistic materials is one of the most interesting topics in biodeterioration nowadays. Pathologies and illnesses of organic and inorganic materials provoked by microorganisms can be treated by experts by a variety of preventive interventions. Artistic medicine encompasses the monitoring of the exhibition and storage of art, as well as proper environmental conditions and the regular cleaning of museums. Biodeterioration control is essential in order to prevent fungal and bacterial contamination in artwork. Biodeterioration of canvas paintings is a complex phenomenon, not well-known at the moment. Canvas paintings are created by several artistic techniques on textile supports that are not always kept in the best conditions, and the best parameters of preventive conservation are often not applied. Therefore, we need to research the agents and the main causes that provoke canvas painting biodeterioration. By applying new methodologies, we can identify the alterations and the treatments needed in order to manage the diverse materials employed in artwork correctly. Herein, we review the causes of biodeterioration that affect artwork, especially art created on textile supports. We also study the alterations of the natural filmogenic materials employed in traditional pictorial techniques, such as agglutinants and protection layers, and the biodeterioration agents that impact them. Additionally, we review current scientific methods employed for the identification of microbial species, and the types of alterations of the materials where the organisms grow. Finally, we summarize the different biocides and preventive conservation treatments that are currently employed.

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Identification of bacterial cultures from archaeological wood using molecular biological techniques

Cited by 33 publications

References 25 publications

Bacterial and Biodeterioration Analysis of the Waterlogged Wooden Lacquer Plates from the Nanhai No. 1 Shipwreck

Bacterial and Biodeterioration Analysis of the Waterlogged Wooden Lacquer Plates from the Nanhai No. 1 Shipwreck

Archaeological Wood

Physiology of biodeterioration on canvas paintings

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