Identification of autonomic neuronal chains innervating gingiva and lip

Szabó, Enikő; Csáki, Ágnes; Boldogkői, Zsolt; Tóth, Zsófia; Köves, Katalin

doi:10.1016/j.autneu.2015.03.005

Cited by 10 publications

(10 citation statements)

References 54 publications

(49 reference statements)

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“…Additionally, this virus is the most popular multisynaptic tracer of neural circuits [ 9 , 10 , 11 , 12 ]. PRV has been genetically modified in order to restrict its spread exclusively in a retrograde manner [ 10 , 13 , 14 ], to decrease its virulence [ 15 ], or to contain fluorescent protein genes for enhancing detectability of virally infected cells [ 16 ], or acting as fluorescent activity markers, which allow the simultaneous monitoring of the activity of multiple neurons using optical methods [ 17 ]. Finally, PRV has also been utilized as a vector for gene delivery to cardiac muscle cells [ 18 ] and to embryonic spinal cord graft [ 19 ], as well as an oncolytic agent [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome

et al. 2021

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In the last couple of years, the implementation of long-read sequencing (LRS) technologies for transcriptome profiling has uncovered an extreme complexity of viral gene expression. In this study, we carried out a systematic analysis on the pseudorabies virus transcriptome by combining our current data obtained by using Pacific Biosciences Sequel and Oxford Nanopore Technologies MinION sequencing with our earlier data generated by other LRS and short-read sequencing techniques. As a result, we identified a number of novel genes, transcripts, and transcript isoforms, including splice and length variants, and also confirmed earlier annotated RNA molecules. One of the major findings of this study is the discovery of a large number of 5′-truncations of larger putative mRNAs being 3′-co-terminal with canonical mRNAs of PRV. A large fraction of these putative RNAs contain in-frame ATGs, which could initiate translation of N-terminally truncated polypeptides. Our analyses indicate that CTO-S, a replication origin-associated RNA molecule is expressed at an extremely high level. This study demonstrates that the PRV transcriptome is much more complex than previously appreciated.

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Section: Introductionmentioning

confidence: 99%

An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome

et al. 2021

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“…4). This was supported by the findings that VIP immunoreactivity is observed in the otic and submandibular ganglion and in the nerve fibers that innervate the blood vessels in the lip [12,26]. Furthermore, the intravenous administration of VIP also induces vasodilation in the masseter muscle [14] and submandibular gland [15], which is markedly suppressed by a selective VIP receptor antagonist.…”

Section: Discussionmentioning

confidence: 66%

Effect of the parasympathetic vasodilation on temperature regulation via trigeminal afferents in the orofacial area

et al. 2020

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The skin temperature (T m) of the orofacial area influences orofacial functions and is related to the blood flow (BF). Marked increases in BF mediated by parasympathetic vasodilation may be important for orofacial T m regulation. Therefore, we examined the relationship between parasympathetic reflex vasodilation and orofacial T m in anesthetized rats. Electrical stimulation of the central cut end of the lingual nerve (LN) elicited significant increases in BF and T m in the lower lip. These increases were significantly reduced by hexamethonium, but not atropine. VIP agonist increased both BF and T m in the lower lip. The activation of the superior cervical sympathetic trunk (CST) decreased BF and T m in the lower lip; however, these decreases were significantly inhibited by LN stimulation. Our results suggest that parasympathetic vasodilation plays an important role in the maintaining the hemodynamics and T m in the orofacial area, and that VIP may be involved in this response.

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“…There are no data in the literature that indicate that SON does directly send premotor fibers to the autonomic centers. We have never observed labeling in this nucleus after the gingiva, lip [14], and adrenal inoculations (unpublished data). The finding that an interruption of the right optic nerve (the retrograde virus was injected into the right eye) prevented the labeling in the SON, but not in the PVN, strongly supports the above-mentioned observation.…”

Section: Discussionmentioning

confidence: 84%

“…In our previous experiments, we have found virus-labeled neurons not only in the pPVN but in the mPVN after injection of a retrograde traveling virus into some peripheral organs such as the mammary gland [13], lower gingiva, or lower lip of rats [14]. There is also considerable evidence that axons of the PVN neurons branch and innervate neurons that are located in at least two different places [15,16].…”

Section: Discussionmentioning

confidence: 94%

“…These cell groups regulate the entire sympathetic outflow. In our previous experiment, it was shown that a retrograde spreading virus administered to the mammary gland [13], lower gingiva, or lower lip [14] appeared in the corresponding portion of the IML and brain stem and some oxytocinergic and vasopressinergic neurons of the PVN. Gerendai and her coworkers [15] described PVN neurons that simultaneously innervate the left adrenal gland and the left ovary.…”

Section: Introductionmentioning

confidence: 94%

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The Same Magnocellular Neurons Send Axon Collaterals to the Posterior Pituitary and Retina or to the Posterior Pituitary and Autonomic Preganglionic Centers of the Eye in Rats

et al. 2021

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In rats, some parvocellular paraventricular neurons project to spinal autonomic centers. Using the virus tracing technique, we have demonstrated that some magnocellular paraventricular neurons, but not supraoptic neurons, also project to autonomic preganglionic centers of the mammary gland, gingiva, or lip. A part of these neurons has shown oxytocin immunoreactivity. In the present experiment, we have examined whether the same magnocellular neuron that sends fibers to the retina or autonomic preganglionic centers of the eye also projects to the posterior pituitary. Double neurotropic viral labeling and oxytocin immunohistochemistry were used. After inoculation of the posterior pituitary and the eye with viruses, spreading in a retrograde direction and expressing different fluorescence proteins, we looked for double-labeled neurons in paraventricular and supraoptic nuclei. Double-labeled neurons were observed in non-sympathectomized and cervical-sympathectomized animals. Some double-labeled neurons contained oxytocin. After the optic nerve was cut, the labeling did not appear in the supraoptic nucleus; however, it could still be observed in the paraventricular nucleus. In the paraventricular nucleus, the double-labeled cells may be the origin of centrifugal visual fibers or autonomic premotor neurons. In the supraoptic nucleus, all double-labeled neurons are cells of origin of centrifugal visual fibers.

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Identification of autonomic neuronal chains innervating gingiva and lip

Cited by 10 publications

References 54 publications

An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome

An Integrated Sequencing Approach for Updating the Pseudorabies Virus Transcriptome

Effect of the parasympathetic vasodilation on temperature regulation via trigeminal afferents in the orofacial area

The Same Magnocellular Neurons Send Axon Collaterals to the Posterior Pituitary and Retina or to the Posterior Pituitary and Autonomic Preganglionic Centers of the Eye in Rats

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