Identification of AtOPT4 as a Plant Glutathione Transporter

Zhang, Zhong-Chun; Xie, Qingqing; Jobe, Timothy O.; Kau, Andrew R.; Wang, Cun; Li, Yunxia; Qiu, Bao‐Sheng; Wang, Qiuquan; Mendoza‐Cózatl, David G.; Schroeder, Julian I.

doi:10.1016/j.molp.2015.07.013

Cited by 27 publications

(22 citation statements)

References 12 publications

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“…Plants also contain multiple transporters of the oligopeptide transporter family (Lubkowitz 2011). In A. thaliana AtOPT4 and AtOPT6 have both been confirmed as glutathione transporters (Cagnac et al 2004;Koh et al 2002;Pike et al 2009;Wongkaew et al 2018;Zhang et al 2016). Furthermore, the oligopeptide transporters, OsGT1 and BjGT1, from rice (Oryza sativa) and mustard (Brassica juncea) respectively were shown to mediate glutathione transport (Cagnac et al 2004;Zhang et al 2004).…”

Section: Plasma Membrane Glutathione Importers In Yeastmentioning

confidence: 99%

Glutathione: subcellular distribution and membrane transport

Oestreicher

Morgan

2019

Biochem. Cell Biol.

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Glutathione (γ-glutamylcysteinylglycine) is a small tripeptide found at millimolar concentrations in nearly all eukaryotes as well as many prokaryotic cells. Glutathione synthesis is restricted to the cytosol in animals and fungi and to the cytosol and plastids in plants. Nonetheless, glutathione is found in virtually all subcellular compartments. This implies that transporters must exist, which facilitate glutathione transport into and out of the various subcellular compartments. Glutathione may also be exported and imported across the plasma membrane in many cells. However, in most cases, the molecular identity of these transporters remains unclear. Whilst glutathione transport is essential for the supply and replenishment of subcellular glutathione pools, recent evidence supports a more active role for glutathione transport in the regulation of subcellular glutathione redox homeostasis. However, our knowledge of glutathione redox homeostasis at the level of specific subcellular compartments remains remarkably limited and the role of glutathione transport remains largely unclear. In this review we discuss how new tools and techniques have begun to yield insights into subcellular glutathione distribution and glutathione redox homeostasis. In particular, we discuss the known and putative glutathione transporters and examine their contribution to the regulation of subcellular glutathione redox homeostasis.

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Section: Plasma Membrane Glutathione Importers In Yeastmentioning

confidence: 99%

Glutathione: subcellular distribution and membrane transport

Oestreicher

Morgan

2019

Biochem. Cell Biol.

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“…For example, glutathione transporters are likely to be involved in heavy metal detoxi cation and long-distance sulfur transport, while other OPTs may be involved in long-distance nitrogen transport. Great changes in nitrogen distribution occur at certain stages of plant life, such as seed formation, senescence and germination, while OPT transporters provide a potentially energy-e cient and rapid way to move large amounts of amino acids [4][5][6][7][8][9]. Phylogenetic analysis has shown that the OPT family has two different branches, called the yellow stripe (YS) branch and the peptide transport (PT) branch.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Expansion and Expression Analysis of the Oligopeptide Transporter Gene Family Between Cucurbita Moschata and Cucurbita Maxima

Shen¹,

Yuan²

2020

Preprint

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Background: Pumpkin is an important non-saline economic vegetable, salt stress often restricts the growth of pumpkin roots and the transportation and balance of mineral ions in the body. Oligopeptide transporter (OPT) plays an important role in transporting small peptides, secondary amino acids, Glutathione and minerals. However, information about the family of OPT in pumpkin is still limited. Results: In this study, 45 OPT transporters were identified from two cultivated species of Cucurbita moschata and Cucurbita maxima. Phylogenetic analysis showed that these OPT families were divided into two evolutionary branches: OPT clade and yellow stripe-like (YSL) clade. All of these genes contain the typical structure of OPT superfamily, OPT clade contains 12 conserved motifs, while the YSL clade contains 7 conserved motifs. There are tandem gene replication events on chromosomes 13, 16 and 18 of Cucurbita moschata and Cucurbita maxima, and 17 and 18 pairs of genes were collinear with Arabidopsis thaliana, respectively. Promoter element analysis showed that there were many cis-acting elements in the upstream promoters of these genes in Cucurbita moschata and Cucurbita maxima, which responded to 10 kinds of stress, especially hormones (MeJA and ABA) and hypoxia. The expression patterns based on transcriptome data sources showed that some OPT genes were organ-specific and tissue-specific, which might be involved in plant functional development. Transcriptome and qRT-PCR verification tests showed that CmoYSL7, CmaOPT15 and CmaYSL7 of Cucurbita moschata and Cucurbita maxima might play a key role in regulating the balance of metal ions between leaf mesophyll and leaf veins under salt stress. Conclusions: Overall, the data obtained from our study contribute to a better understanding of the complexity of the OPT genes family in pumpkins. These results will provide new insights into the mechanism of salt tolerance and the structure and function of OPT genes in pumpkin.

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“…Enormous amount of mRNAs have been discovered to be mobile during nutrient stress. For example, a total of 3,546 distinct mRNAs were identi ed to be transported from a cucumber (Cucumis sativus) scion to a watermelon (Citrullus lanatus) stock in response to phosphorous de ciency 19 . In another heterografting experiment using two Arabidopsis thaliana ecotypes (COL and PED), although 2,006 different mRNAs were discovered to be mobile in the shoot-root system under normal conditions, only 90 and 91 transcripts were found to be mobile in response to phosphorus and nitrogen de ciency, respectively 20 .…”

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confidence: 99%

The long-distance OPT3 movement is selective between herbaceous and woody plants as shown using Malus and Arabidopsis thaliana plants

Lv¹,

Sun²,

Hao³

et al. 2020

Preprint

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Long-distance mobile mRNAs play key roles in gene regulatory networks controlling plant development and stress resistance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that MdOPT3 mRNA can be transported over a long distance, from the leaf to the root, to regulate Fe uptake; whereas, AtOPT3, the mRNA of the MdOPT3 homolog from Arabidopsis thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 moved from the shoot to the root in Malus and Populus plants, while the mobile MdOPT3 became immobile in A. thaliana and Solanum lycopersicum. Furthermore, we demonstrate that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by the divergent RNA-binding proteins (RBPs) located in herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process.

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Identification of AtOPT4 as a Plant Glutathione Transporter

Cited by 27 publications

References 12 publications

Glutathione: subcellular distribution and membrane transport

Glutathione: subcellular distribution and membrane transport

Evolutionary Expansion and Expression Analysis of the Oligopeptide Transporter Gene Family Between Cucurbita Moschata and Cucurbita Maxima

The long-distance OPT3 movement is selective between herbaceous and woody plants as shown using Malus and Arabidopsis thaliana plants

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