Identification of antihypertensive peptides from mung bean protein hydrolysate and their effects in spontaneously hypertensive rats

Sonklin, Chanikan; Alashi, Monisola A.; Laohakunjit, Natta; Kerdchoechuen, Orapin; Aluko, Rotimi E.

doi:10.1016/j.jff.2019.103635

Cited by 79 publications

(77 citation statements)

References 56 publications

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“…The renin-inhibitory activity of samples was determined using a previously described method [ 26 ]. Briefly, 20 µL of the substrate, 160 µL of assay buffer, and 10 µL of distilled water were mixed and added to the background well.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Sultana

Alashi

Islam

et al. 2020

Foods

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The aim of the study was to determine the in vitro enzyme inhibition activities of aqueous polyphenolic extracts of nine popular Bangladeshi vegetables, namely ash gourd, bitter gourd, brinjal, Indian spinach, kangkong, okra, ridge gourd, snake gourd, and stem amaranth. Polyphenolic glycosides were the major compounds present in the extracts. Inhibition of α-amylase (up to 100% at 1 mg/mL) was stronger than α-glucosidase inhibition (up to 70.78% at 10 mg/mL). The Indian spinach extract was the strongest inhibitor of pancreatic lipase activity (IC50 = 276.77 µg/mL), which was significantly better than that of orlistat (381.16 µg/mL), a drug. Ash gourd (76.51%), brinjal (72.48%), and snake gourd (66.82%) extracts were the most effective inhibitors of angiotensin-converting enzyme (ACE), an enzyme whose excessive activities have been associated with hypertension. Brinjal also had a significantly higher renin-inhibitory activity than the other vegetable extracts. We conclude that the vegetable extracts may have the ability to reduce enzyme activities that have been associated with hyperglycemia, hyperlipidemia, and hypertension.

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Section: Methodsmentioning

confidence: 99%

Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Sultana

Alashi

Islam

et al. 2020

Foods

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“…According to Dianzhi Hou et al [ 113 ], small mung bean peptides with hydrophobic amino acid residues show higher bioactivity. Additionally, YADLVE also reduces blood pressure to up to 36 mmHg over a 24 h period after it is administered orally to spontaneously hypertensive rats [ 114 ]. Mung bean protein hydrolysate exerts strong and dose-dependent suppressing effects on proinflammatory mediators by blocking the nuclear factor-kappa B (NF-κB) pathway in liposaccharide (LPS)-stimulated RAW 264.7 macrophages [ 115 ].…”

Section: Production Characterization and Health Impact Of Mung Bmentioning

confidence: 99%

Health Benefits of Antioxidative Peptides Derived from Legume Proteins with a High Amino Acid Score

2021

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Legumes such as soybean, chickpea, lentil, cowpea, and mung bean, are valuable sources of protein with a high amino acid score and can provide bioactive peptides. This manuscript presents a review on legume-derived peptides, focusing on in vitro and in vivo studies on the potential antioxidative activities of protein hydrolysates and their characterization, amino acid sequences, or purified/novel peptides. The health implications of legume-derived antioxidative peptides in reducing the risks of cancer and cardiovascular diseases are linked with their potent action against oxidation and inflammation. The molecular weight profiles and amino acid sequences of purified and characterized legume-derived antioxidant peptides are not well established. Therefore, further exploration of legume protein hydrolysates is necessary for assessing the potential applications of antioxidant-derived peptides in the functional food industry.

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“…However, these peptide and amino acids could play various functional properties and bioactive activities, including the ability to act as antioxidants. Previous studies have reported other bioactive properties in mung bean peptides and protein hydrolysates such as antihypertensive [ 23 , 24 ], anti-allergic [ 25 ], and anti-tumor [ 26 ]. However, only few studies have reported the antioxidant activities of specific peptide sequences derived from mung bean protein hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Mung Bean Meal Protein-Derived Antioxidant Peptides

et al. 2021

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The aim of this work was to characterize the antioxidant properties of some of the peptides present in bromelain mung bean meal protein hydrolysate (MMPH). The MMPH was subjected to two rounds of bioassay-guided reversed-phase HPLC separation followed by peptide identification in the most potent fractions using tandem mass spectrometry. Twelve antioxidant peptides, namely, HC, CGN, LAN, CTN, LAF, CSGD, MMGW, QFAAD, ERF, EYW, FLQL, and QFAW were identified and assayed for antioxidant properties. CTN, HC, CGN, and CSGD were the most potent (p < 0.05) DPPH radical scavengers with EC50 values of 0.30, 0.29, 0.28, and 0.30 mg/mL, respectively, which are lower than the 0.03 mg/mL obtained for reduced glutathione (GSH). CTN, HC, CGN, and CSGD exhibited the most potent (p < 0.05) scavenging activities against hydroxyl and superoxide radicals with EC50 values that are similar to those of GSH. The cysteine-containing peptides also had stronger ferric reducing antioxidant power and metal chelation activity than peptides devoid of cysteine. In contrast, MMGW, ERF, and EYW had poor radical scavenging and metal chelation activities. We conclude that the availability of the sulfhydryl group may have enhanced antioxidant potency while the presence of bulky groups such phenylalanine and tryptophan had an opposite effect.

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Identification of antihypertensive peptides from mung bean protein hydrolysate and their effects in spontaneously hypertensive rats

Cited by 79 publications

References 56 publications

Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Health Benefits of Antioxidative Peptides Derived from Legume Proteins with a High Amino Acid Score

Functional Characterization of Mung Bean Meal Protein-Derived Antioxidant Peptides

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