Identification of antigenic proteins from salivary glands of female  Anopheles maculatus  by proteomic analysis

Armiyanti, Yunita; Arifianto, Renam Putra; Riana, Elisa Nurma; Senjarini, Kartika; Widodo, Widodo; Fitri, Loeki Enggar; Sardjono, Teguh Wahju

doi:10.1016/j.apjtb.2016.08.012

Cited by 10 publications

(7 citation statements)

References 16 publications

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“…Anopheles stephensi mosquito is the vector of malaria transmission to the human being in the Indian subcontinent. While the salivary gland proteins of Anopheles mosquito was reported for their role in parasite pathogenesis and having the ability to induce IgG response in the natural host 20 , 21 . Therefore, total 33 salivary proteins of A. stephensi were obtained from the national center for biotechnology information (NCBI) protein database (Retrieval date 25/08/2017) and subjected to multi-epitope vaccine designing.…”

Section: Methodsmentioning

confidence: 99%

“…reported that salivary proteins might be utilized to develop novel antimalarial control strategies via innate immune protection against malaria 16 . These proteins could likewise evoke a host IgG response in natural conditions 20 , 21 . Mosquito salivary gland surface (SGS) proteins are the prevalent immunogenic component present in saliva having ability to induce immunogenic responses 22 .…”

Section: Introductionmentioning

confidence: 99%

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Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Pandey

Bhatt

Prajapati

2018

Sci Rep

194

165

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Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course of biting, a salivary protein of Anopheles helps in blood feeding behavior and having the ability to elicit the host immune response. This study represents a series of immunoinformatics approaches to design multi-epitope subunit vaccine using Anopheles mosquito salivary proteins. Designed subunit vaccine was evaluated for its immunogenicity, allergenicity and physiochemical parameters. To enhance the stability of vaccine protein, disulfide engineering was performed in a region of high mobility. Codon adaptation and in silico cloning was also performed to ensure the higher expression of designed subunit vaccine in E. coli K12 expression system. Finally, molecular docking and simulation study was performed for the vaccine protein and TLR-4 receptor, to determine the binding free energy and complex stability. Moreover, the designed subunit vaccine was found to induce anti-salivary immunity which may have the ability to prevent the entry of Plasmodium sporozoites into the human host.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Pandey

Bhatt

Prajapati

2018

Sci Rep

194

165

View full text Add to dashboard Cite

show abstract

“…Immunological diversity becomes quite relevant when we consider establishment of "markers of exposure"essential tools for the determination of exposure to vector bites. Individuals exposed to vector bites show different patterns of antibody binding to salivary proteins (Gomes et al, 2002;Vinhas et al, 2007;Armiyanti et al, 2016). Some salivary proteins are recognized only by the sera of a few individuals.…”

Section: Markers Of Exposure: Sand Fly Salivary Proteins As Tools For...mentioning

confidence: 99%

Some Good and Some Bad: Sand Fly Salivary Proteins in the Control of Leishmaniasis and in Autoimmunity

Aoki

Abdeladhim

et al. 2022

Front. Cell. Infect. Microbiol.

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Sand flies are hematophagous insects responsible for the transmission of vector-borne diseases to humans. Prominent among these diseases is Leishmaniasis that affects the skin and mucous surfaces and organs such as liver and spleen. Importantly, the function of blood-sucking arthropods goes beyond merely transporting pathogens. The saliva of vectors of disease contains pharmacologically active components that facilitate blood feeding and often pathogen establishment. Transcriptomic and proteomic studies have enumerated the repertoire of sand fly salivary proteins and their potential use for the control of Leishmaniasis, either as biomarkers of vector exposure or as anti-Leishmania vaccines. However, a group of specific sand fly salivary proteins triggers formation of cross-reactive antibodies that bind the ectodomain of human desmoglein 1, a member of the epidermal desmosomal cadherins. These cross-reactive antibodies are associated with skin autoimmune blistering diseases, such as pemphigus, in certain immunogenetically predisposed individuals. In this review, we focus on two different aspects of sand fly salivary proteins in the context of human disease: The good, which refers to salivary proteins functioning as biomarkers of exposure or as anti-Leishmania vaccines, and the bad, which refers to salivary proteins as environmental triggers of autoimmune skin diseases.

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“…[1][2][3][4] Anopheles maculatus was a malaria vector in Thailand, the Philippines, Malaysia 5,6 , Afghanistan, Pakistan, China, and India. 7 The potential of mosquitoes as malaria vectors can be seen from their behavior. The results of research in Thailand and Purworejo Central Java, Indonesia; An.…”

Section: Introductionmentioning

confidence: 99%

Distribution and behavior of Anopheles maculatus and its potential as a Malaria vector in Indonesia

Setiyaningsih¹,

Prihasto²,

Ayuningrum³

et al. 2023

Epidemiol. Soc. Heal. Rev.

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Background: Anopheles maculatus is one of the mosquito species that has been confirmed as a malaria vector in Indonesia. The potential of a mosquito as a vector is influenced by its behavior. Information on the distribution and behavior of An. maculatus needs to be carried out to determine the potential for malaria transmission transmitted by the species in an area. The study aimed to obtain information on the distribution, behavior, and potential of maculatus as a malaria vector in several provinces in Indonesia. Method: Mosquito collection was carried out using human-landing collection, animal-baited trap, cattle-bait, light-trap, and resting morning. A survey of mosquitoes was carried out in 29 provinces in Indonesia. Mosquitoes were identified for the species and detected the blood-sucking behavior with an Enzyme-linked immunosorbent assay and the presence of Plasmodium using a Polymerase Chain Reaction. Results: The results showed that An. maculatus was found in the Riau Islands, Lampung, Bangka Belitung, West Java, Central Java, East Java, and Central Sulawesi. Anopheles maculatus has known to suck the blood of humans and animals with a predominance of animals. Anopheles maculatus is also known to suck blood outdoors predominantly. In general, the activity of An. maculatus sucking blood begins around 18.00 in the evening. Central Java Province was the province with the highest density of An. maculatus mosquitoes, thereby increasing the potential for transmission of malaria cases. Conclusion: Anopheles maculatus was spread in Riau Islands, Lampung, Bangka Belitung, West Java, Central Java, East Java, and Central Sulawesi. This species was known to suck the blood of people and humans, and its blood-sucking activity starts around 18.00 in the evening.

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Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis

Cited by 10 publications

References 16 publications

Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Some Good and Some Bad: Sand Fly Salivary Proteins in the Control of Leishmaniasis and in Autoimmunity

Distribution and behavior of Anopheles maculatus and its potential as a Malaria vector in Indonesia

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