Identification of antifungal substances secreted by Bacillus subtilis Z-14 that suppress Gaeumannomyces graminis var. tritici

Inhibitory Abilities of Bacillus Isolates and Their Culture Filtrates against the Gray Mold Caused by Botrytis cinerea on Postharvest Fruit

“…Secondary metabolites play key roles in the antifungal activities of antagonistic bacteria; however, the production of these antagonistic metabolites is greatly affected by the environment (Zhang et al, 2017a). The direct use of antimicrobial substances synthesized by microorganisms as the basis of microbial pesticides can overcome such limitations (Kilani-Feki et al, 2016).…”

“…Bacterial isolate RS-25 from jujube fruit, MG-4 from strawberry fruit, Z-14 from wheat rhizosphere soil and Pnf-4 from wheat plant significantly inhibited the growth of various phytopathogenic fungi and were maintained at 4°C on streak-inoculating nutritive agar (NA) slants (Zhang et al, 2017a, 2017b). The strains were cultured overnight at 37°C on NA slants, transferred to Erlenmeyer flasks containing 50 ml of the seed culture medium, and shaken at 220 rpm for 48 h at 37°C.…”

Novel screening strategy reveals a potent Bacillus antagonist capable of mitigating wheat take-all disease caused by Gaeumannomyces graminis var. tritici

“…After storage overnight at 4°C, the mixture was again centrifuged to isolate the precipitate, which was then washed twice with diluted HCl (pH 2·0) and extracted twice by using methanol (Zhang et al . ). This methanol extract underwent further detection by mass spectrometry in a MALDI‐TOF mass spectrometer (Ettan MALDITOF ProSystem, Amersham Biosciences, Sweden), which operated in the reflectron mode and used α‐cyano‐4‐hydroxycinnamic acid as the matrix (Velho et al .…”

Purification and heterologous expression of a novel antifungal protein from Bacillus subtilis strain Z-14

“…Strains and culture conditions B. subtilis Z-14 (hereafter referred to as Z-14), which signi cantly reduced the growth of Rhizoctonia cerealis (a wheat sheath blight pathogen), was originally isolated from soil sampled from a wheat rhizosphere [20]. Z-14 was grown on nutritive agar (NA) overnight at 37 °C.…”

“…These proteins might be important to protect plants from pathogen infection. B. subtilis Z-14, selected from a wheat rhizosphere soil, demonstrated broad spectrum activity against phytopathogenic fungi in vivo and in vitro [20,21]. The present study aimed to purify the novel antifungal protein secreted by strain Z-14, express it heterologously, and perform a preliminary characterization.…”