Identification of antibiotic-resistant Escherichia coli isolated from a municipal wastewater treatment plant

Zanotto, Carlo; Bissa, Massimiliano; Illiano, Elena; Mezzanotte, Valeria; Marazzi, Francesca; Turolla, Andrea; Antonelli, Manuela; Morghen, Carlo De Giuli; Radaelli, Antonia

doi:10.1016/j.chemosphere.2016.08.040

Cited by 39 publications

(26 citation statements)

References 36 publications

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“…The mean reduction values of the faecal indicators at specific PAA doses are reported in Figure 1. During the monitoring, high concentrations [2,28] of faecal coliforms and E. coli, as well as enterococci and C. perfringens were observed in raw sewage ( Table 4). The concentrations of the four bacterial indicators were similar in the summer, autumn, and spring (range: 2.91 to 4.42 log MPN/100 mL or CFU/100 mL), whereas a major contamination was observed during the winter (range: 4.21 to 5.74 log CFU-MPN/100 mL or CFU/100 mL).…”

Section: Salmonella E Coli O157:h7 Campylobactermentioning

confidence: 99%

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Peracetic Acid (PAA) Disinfection: Inactivation of Microbial Indicators and Pathogenic Bacteria in a Municipal Wastewater Plant

Bonetta¹,

Pignata²,

Lorenzi

et al. 2017

Water

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Abstract:Several studies have noted that treated and untreated wastewaters are primary contributors of a variety of pathogenic microorganisms to the aquatic ecosystem. Conventional wastewater treatment may not be sufficient to achieve microbiologically safe effluent to be discharged into natural waters or reused, thus requiring wastewater effluents to be disinfected. In recent years, peracetic acid (PAA) has been adopted as a disinfectant for wastewater effluents. The aim of this study was to evaluate the disinfection efficiency of PAA at low doses (range 0.99-2.10 mg/L) against microbial indicators and pathogenic bacteria in a municipal wastewater plant. Samples of untreated sewage and effluents before and after PAA treatment were collected seasonally for 1 year and were analysed for pathogenic Campylobacter, Salmonella spp., E. coli O157:H7 and E. coli virulence genes using molecular methods; moreover, the detection of specific microbial indicators (E. coli, faecal coliforms, enterococci, C. perfringens) and Salmonella spp. were carried out using culturing methods. Salmonella spp. DNA was found in all untreated sewage and effluent before PAA treatment, whereas it was recovered in 50% of the samples collected after PAA treatment. Although E. coli O157:H7 was never identified, the occurrence of Shiga-like toxin I amplicons was identified in 75% of the untreated sewage samples, in 50% of the effluents assayed before PAA treatment, and in 25% of the effluents assayed after PAA treatment, whereas the stx2 gene was never found. Campylobacter coli was only detected in one effluent sample before PAA treatment. In the effluents after PAA treatment, a lower load of indicator bacteria was observed compared to the effluents before treatment. The results of this study highlight that the use of low doses of PAA seems to lead to an improvement of the microbiological quality of the effluent, although it is not sufficient to guarantee its suitability for irrigation. These results underscore the need for additional studies to further assess the efficiency of PAA disinfection in municipal wastewater plants.

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Section: Salmonella E Coli O157:h7 Campylobactermentioning

confidence: 99%

“…Zanotto et al [28] detected ampicillin-and chloramphenicol-resistant E. coli in the influent and effluent from a municipal WWTP in the Milan area (Italy). The WWTP applied a final disinfection with PAA (approximately 2.0 mg/L, 45 min contact time).…”

Section: Salmonella E Coli O157:h7 Campylobactermentioning

confidence: 99%

Peracetic Acid (PAA) Disinfection: Inactivation of Microbial Indicators and Pathogenic Bacteria in a Municipal Wastewater Plant

Bonetta¹,

Pignata²,

Lorenzi

et al. 2017

Water

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“…Moreover, dissemination of ARGs may also be affected by wastewater contamination with heavy metals, whose residues may pose co-selection pressure on ARG distribution [24]. For this reason, ARGs are not effectively eliminated in the wastewater treatment process and their concentration may even increase [39]. Wastewater discharge to the environment or their re-utilisation may lead to the dissemination of ARB and ARGs in the environment, threatening the ecological safety of aquatic ecosystems, in particular [27,40].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge

et al. 2019

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Wastewater treatment plants (WWTPs) are an important reservoir in the development of drug resistance phenomenon and they provide a potential route of antibiotic resistance gene (ARGs) dissemination in the environment. The aim of this study was to assess the role of WWTPs in the spread of ARGs. Untreated and treated wastewater samples that were collected from thirteen Polish WWTPs (applying four different modifications of activated sludge–based treatment technology) were analyzed. The quantitative occurrence of genes responsible for the resistance to beta-lactams and tetracyclines was determined using the real-time PCR method. Such genes in the DNA of both the total bacterial population and of the E. coli population were analyzed. Among the tested genes that are responsible for the resistance to beta-lactams and tetracyclines, blaOXA and blaTEM and tetA were dominant, respectively. This study found an insufficient reduction in the quantity of the genes that are responsible for antibiotic resistance in wastewater treatment processes. The results emphasize the need to monitor the presence of genes determining antibiotic resistance in the wastewater that is discharged from treatment plants, as they can help to identify the hazard that treated wastewater poses to public health.

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“…However, rapid decrease of ARB was observed after the disinfection with only 8 μg/ml ampicillin. About 78% of the resistant E. Coli were reported resistant to 32 μg/ml ampicillin [17]. Studies of a certain WWTP in Jedah, Saudi Arabia have also been reported.…”

Section: Arb Occurrence In Wwtpmentioning

confidence: 99%

Threats from antibiotics: A serious environmental concern

Inyinbor

Bello

Fadiji

et al. 2018

Journal of Environmental Chemical Engineering

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Antibiotics are bioactive substances, used as human and animal medicines for illness prevention, disease treatment and growth promotion. They are considered to be pseudo persistent given their continuous input in the environment. Antibiotics enter agro ecosystems through several routes such as wastewater irrigation, soil application, animal manures or bio-solids which are mostly biologically active thus creating potential risks to the environment. They are present in different environmental matrices at low concentrations as residues. Antibiotic residues enter the environment primarily via urine, feces and manure from humans and animals after they have taken the medication, as well as from manufacturing wastewater. These residues contaminate the soil, surface water, and groundwater by leaching or runoff and ultimately breed antibiotic-resistant bacteria (ARB) and genes (ARG). These triune threat viz antibiotics, ARB and ARG have not been effectively removed by various treatment in wastewater treatment plants. Here, we put together existing knowledge and aim at providing in-depth knowledge to the extent to which a wide range of treatment processes determine the ultimate fate of antibioticbred threats (ARB and ARG) in conventional and advanced wastewater treatment. The use of antibiotics is inevitable, hence studies focusing on minimizing their discharge into the environment viz-a-viz support future regulatory measures are of great importance.

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Identification of antibiotic-resistant Escherichia coli isolated from a municipal wastewater treatment plant

Cited by 39 publications

References 36 publications

Peracetic Acid (PAA) Disinfection: Inactivation of Microbial Indicators and Pathogenic Bacteria in a Municipal Wastewater Plant

Peracetic Acid (PAA) Disinfection: Inactivation of Microbial Indicators and Pathogenic Bacteria in a Municipal Wastewater Plant

Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge

Threats from antibiotics: A serious environmental concern

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