2001
DOI: 10.1007/s007050170115
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Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection

Abstract: Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enzyme-linked immunosorbent assay (ELISA) using experimental antisera collected from pigs inoculated with a PCV2 isolate. Two epitopes spanning residues 69 to 83 and 117 to 131 were specific to PCV2. Antibodies to the 1… Show more

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Cited by 56 publications
(40 citation statements)
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References 21 publications
(35 reference statements)
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“…Previous studies of humoral immunity during PCV2 infection have primarily focused on mapping antigenic regions of CP, including the characterization of immunorelevant epitopes (26,33,55). The present study describes differences in the antibody responses toward PCV2 CP immunoreactive regions following vaccination and experimental infection and during severe disease.…”
Section: Discussionmentioning
confidence: 85%
See 1 more Smart Citation
“…Previous studies of humoral immunity during PCV2 infection have primarily focused on mapping antigenic regions of CP, including the characterization of immunorelevant epitopes (26,33,55). The present study describes differences in the antibody responses toward PCV2 CP immunoreactive regions following vaccination and experimental infection and during severe disease.…”
Section: Discussionmentioning
confidence: 85%
“…Previous immunological studies identified several antibody recognition sites in CP, including at least one immunorelevant epitope (26,33,55). The epitope recognition regions from these previous studies were combined and summarized in Fig.…”
Section: Experimental Pcv2 Infectionmentioning
confidence: 99%
“…The PCV2 genome comprises 1767 or 1768 nt and is assumed to have 11 potential open reading frames (ORF1-11; Hamel et al, 1998;Zhou et al, 2006). Previous studies have demonstrated that ORF1, -2 and -3 encode a 35.7 kDa replication (Rep) protein involved in virus replication (Mankertz et al, 1998), a 27.8 kDa capsid (Cap) protein involved in PCV2 immunogenicity (Mahe et al, 2000;Nawagitgul et al, 2000;Truong et al, 2001;Zhou et al, 2005a) and a protein involved in PCV2-induced apoptosis , respectively.…”
Section: Introductionmentioning
confidence: 99%
“…The PCV2 genome comprises 1767 or 1768 nt and is assumed to have 11 potential open reading frames (ORF1-11;Hamel et al, 1998;Zhou et al, 2006). Previous studies have demonstrated that ORF1, -2 and -3 encode a 35.7 kDa replication (Rep) protein involved in virus replication (Mankertz et al, 1998), a 27.8 kDa capsid (Cap) protein involved in PCV2 immunogenicity (Mahe et al, 2000; Nawagitgul et al, 2000;Truong et al, 2001;Zhou et al, 2005a) and a protein involved in PCV2-induced apoptosis , respectively.Immunization against PCV2 has been studied intensely and found to be the most effective strategy for protecting pigs against PCV2 infection. Based on comparison of the immunogenicity of the PCV2 Cap and Rep proteins, Blanchard et al (2003) demonstrated that the ORF2-encoded Cap protein was the major immunogen, whilst the ORF1-encoded Rep protein was only weakly immunogenic.…”
mentioning
confidence: 99%
“…The existence of common immunoreactivity epitope on the ORF1-encoded protein, but the lack of cross-reactivity between the ORF2-encoded proteins in both types of PCV has been described previously [7,15]. In fact, no cross-reactivity was found in the antisera against PCV1 by the rcELISA.…”
Section: Discussionmentioning
confidence: 81%