Antibody Recognition of Porcine Circovirus Type 2 Capsid Protein Epitopes after Vaccination, Infection, and Disease

Trible, Benjamin R.; Kerrigan, Maureen; Crossland, Nicholas A.; Potter, M. L.; Faaberg, Kay S.; Hesse, Richard; Rowland, Raymond R. R.

doi:10.1128/cvi.00418-10

Cited by 77 publications

(86 citation statements)

References 54 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, a small number of mutations may result in increased pathogenicity. The amino acid sequence positions 173-175 and 179 are important for antibody recognition [36]. Our results showed that all of the amino acids at these positions were relatively conserved among PCV2a and 2b, and new emerging viruses (Fig.…”

Section: Discussionmentioning

confidence: 60%

See 1 more Smart Citation

Genotypic Change and Phylogenetic Analysis of Porcine Circovirus Type 2 in Taiwanese Pig Herds

Chiou

Lin

Yang

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome. Two major PCV2 genotypes, PCV2a and PCV2b, have been identified. To explore the prevalence of different subgroups of PCV2 in Taiwan Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases (PCVADs) in pigs and causes significant economic losses in most pig-producing countries [27]. PCVADs can be subclinical or can include 1 or more clinical manifestations such as multisystemic disease with weight loss, respiratory signs, enteric signs, porcine dermatitis and nephropathy syndrome, and reproductive problems individually or in combination in pigs raised on farms or in other groups of pigs [27]. PCVADs are multifactorial syndromes, and factors that are currently thought to influence the outcome of PCV2 infection include the viral strain, the host, coinfections, and immune modulation [27].PCV2 is a small, non-enveloped, circular, single-stranded DNA virus containing a circular genome of 1766-1768 nucleotides [17,34]. PCV2 is a member of the genus Circovirus of the family Circoviridae [27]. Another member of this genus includes PCV1, which does not produce disease in pigs [35]. The PCV2 genome contains three open reading frames (ORFs). ORF1 encodes two replicase proteins, Rep and Rep' [4]. The viral capsid protein is encoded by ORF2 [25], which is involved in the host immune response [24]. The third ORF, ORF3, is in a different reading frame embedded within ORF1 and encodes a protein associated with apoptosis [22].PCV2 viruses are further classified into three genotypes (2a, 2b, and 2c) that differ with respect to the ORF2 sequence [9,26]. The signature motif (amino acid positions 86-89) of ORF2 can be used to distinguish PCV2a and 2b, which contain the TNKI and SNPR (L) motifs, respectively [6]. The PCV2c genotype has only been found in Denmark to date [31]. In recent years, several reports have suggested that PCV2b is the predominant genotype in many countries worldwide, including Brazil [8,11], Canada [14,15] [16,32]. In Taiwan, the first PCV2 outbreak was confirmed in 2001 [3], and a high prevalence of PCV2 infection was noted thereafter. Up to 92% of pig herds and 68.8% of weaned pigs were infected by PCV2 in Taiwan [7]; however, the genotype prevalence of PCV2 in Taiwan is not currently known. The aim of the present study was to investigate the prevalence of the PCV2 genotype in Taiwan in the last decade. MATERIALS AND METHODS Specimen collection and histopathological examination:Clinical samples (lungs, lymph nodes, spleen, brain and serum) were collected from 785 pigs from 186 herds in central and southern Taiwan during 2002-2011. These samples were mainly acquired from weaning and growing pigs with

show abstract

Section: Discussionmentioning

confidence: 60%

“…The signature residues at positions 173-175 and 179 are important for antibody recognition [36]. Among our samples, we observed amino acid conservation at these 4 positions (Fig.…”

Section: Resultsmentioning

confidence: 67%

Genotypic Change and Phylogenetic Analysis of Porcine Circovirus Type 2 in Taiwanese Pig Herds

Chiou

Lin

Yang

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Only one of the Abs recognized the C-terminal fragment of Phl p 1, which is immunodominant in humans, and it recognized an epitope different from those recognized by human IgE, as defined by alanine scanning mutagenesis. This suggests that there may be differences in epitopes targeted by Abs derived from naturally developed repertoires and Abs produced by immunization, as previously reported in several other systems (43)(44)(45)(46)(47). Such differences could potentially be explained by a combination of biological processes and technical variations.…”

Section: Discussionmentioning

confidence: 61%

Phl p 1–Specific Human Monoclonal IgE and Design of a Hypoallergenic Group 1 Grass Pollen Allergen Fragment

Levin

Rydnert

Källström

et al. 2013

The Journal of Immunology

View full text Add to dashboard Cite

Detailed understanding of how Abs of the IgE isotype interact with allergen at the onset of an allergic reaction is of great importance for deciphering mechanisms involved in the development of disease and may aid in the design of hypoallergenic variants. In this study, we have used a set of human monoclonal IgE Abs derived from the repertoires of allergic individuals, specific for the major timothy grass pollen allergen Phl p 1, to gain detailed information on the interaction between Abs and allergen. These allergen-specific IgE are to varying degrees cross-reactive toward both different allergen isoforms and various group 1 allergens originating from other grass species. The usage of human monoclonal IgE, as an alternative to polyclonal preparations or mouse Abs, allowed us to locate several important IgE-binding epitopes on the C-terminal domain of Phl p 1, all clustered to an IgE-binding “hot spot.” By introducing three mutations in the IgE-binding area of the C-terminal domain we were able to significantly reduce its reactivity with serum IgE. In conclusion, our study shows the great potential of using human monoclonal IgE as a tool for studies of the molecular interactions taking place during allergic responses. Furthermore, we present a novel IgE-hyporeactive fragment with the potential to be used as a safer hypoallergenic alternative in specific immunotherapy than the pollen extracts used today.

show abstract

“…23 The PRRSV/PCV-2 inoculum was originally prepared from a lymph node derived from a pig with severe postweaning multisystemic wasting syndrome (PMWS). 27,28 Because wild-type PCV-2 subgenotype b does not propagate to high levels in cell culture, we took advantage of the heat stability of PCV-2 to make a virus preparation from a lymph node suspension enriched for PCV-2. The suspension was heat-treated at 55°C for 30 min.…”

Section: Pigs and Virusesmentioning

confidence: 99%

Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs

et al. 2015

Self Cite

View full text Add to dashboard Cite

Abstract. Many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. This study investigated the utility of the Lawrence Livermore Microbial Detection Array (Lawrence Livermore National Laboratory, Livermore, California), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coinfected with Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine circovirus-2 (PCV-2). The array easily identified PRRSV and PCV-2, but at decreased sensitivities compared to standard polymerase chain reaction detection methods. The oral fluid sample was the most informative, possessing additional signatures for several swine-associated bacteria, including Streptococcus sp., Clostridium sp., and Staphylococcus sp.

show abstract

Antibody Recognition of Porcine Circovirus Type 2 Capsid Protein Epitopes after Vaccination, Infection, and Disease

Cited by 77 publications

References 54 publications

Genotypic Change and Phylogenetic Analysis of Porcine Circovirus Type 2 in Taiwanese Pig Herds

Genotypic Change and Phylogenetic Analysis of Porcine Circovirus Type 2 in Taiwanese Pig Herds

Phl p 1–Specific Human Monoclonal IgE and Design of a Hypoallergenic Group 1 Grass Pollen Allergen Fragment

Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs

Contact Info

Product

Resources

About