Identification of an ectopic developmental antigen that appears in malignant ascitic fluid

Nakai, Mitsuo; Ishikawa, Masakazu; Kawauchi, Hirohito

doi:10.1016/0028-2243(91)90162-e

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…In short, it was generated in rabbits and made monospecific withthe same manner as described previously (Kami et al, 1991;Nakai et aL, 1991). The optimal dilution of the primary antiserum was 1 :200.…”

Section: Anti-91kda Protein Antibodymentioning

confidence: 99%

“…In a recent decade, an ectopic pregnancyassociated and tumour-related protein; the 91kDa protein, was isolated as the second-generation antigen from the ascitic fluids of a patient suffered from ovarian and uterine cancers with mixed mesodermal tumours (Kami et al, 1991;Nakai et al, 1991Nakai et al, , 1992. Retrospectively, the protein was found to be cross-reactive with the antibody against the first generation protein; 94kDa-3-protein that was originally separated from the human placenta at term, and confirmed to be a candidate of a novel pregnancy-associated and tumour-related protein (Kami et aL, 1991;Nakai et aL, 1981Nakai et aL, , 1985Nakai et aL, , 1987aNakai et aL, , 1987bNakai et aL, , 1988Nakai et aL, , 1989.…”

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Cellular Characterization of the 91kDa-ectopic Ascitic Antigen Sharing Antigenicity with MRP8 in the Human Placenta as Revealed by Immunoelectron Microscopical Colloidal-gold Techniques

Sato

Morita

Ishiwata

et al. 1998

Okajimas Folia Anatomica Japonica

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Summary: Cellular characterization of the 91kDa-ectopic ascitic protein that exhibits pregnancy-associated and tumourrelated dynamics has been examined in the human placenta using an electron microscopic immunocolloidal-gold technique. This protein was initially isolated from the ascitic fluids of a patient suffered from ovarian and uterine cancers with mixed mesodermal tumours, and determined to be sharing antigenicity with the 28kDa-oncodevelopmental protein and a calcium-binding protein; MRP8/CFA, respectively. Placentas obtained were divided into three groups by their gestational periods. Small chorionic villous tissues were embedded in Lowicryl K4M resin or Epon 812 resin. Specific and higher labellings by gold-particles were obtained in sections of Lowicryl resin and, then, recognized in mesenchyme-derived cells and/or myeloid lineages; such as placental tissue macrophages (Hofbauer cells), fibroblasts, foetal myelomonocytic cells including endothelial cells, etc., in the first and second trimesters. So far, the pattern of antigenic appearances changed depending on the stage of gestation. On the other hand, 91kDa-protein was also determined in the syncytiotrohpoblast, but not in cytotrophoblasts at whenever been examined. It is assumed that the antigenic expression in syncytiotrophoblasts might be reflected to be absorbed or incorporated from those of foetal or maternal origins, and the antibody used in this study should be sensitive to the antigenic epitope derived from those of myeloid lineages. In the light of these results, hypotheses concerning mechanisms of both transplacental permeability of substances by the placental barrier and cell/tissue differentiation by calcium-binding (and/or -depending) proteins such as 91kDa-protein, MRP8, etc.; presumable the S-100 protein family, are discussed further.

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Section: Anti-91kda Protein Antibodymentioning

confidence: 99%

mentioning

confidence: 99%

Cellular Characterization of the 91kDa-ectopic Ascitic Antigen Sharing Antigenicity with MRP8 in the Human Placenta as Revealed by Immunoelectron Microscopical Colloidal-gold Techniques

Sato

Morita

Ishiwata

et al. 1998

Okajimas Folia Anatomica Japonica

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Short Communication

Kasza

Bugno²,

Koj³

1994

Biological Chemistry Hoppe-Seyler

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HepG2 cells were cultured for 7 days in serum-free medium in the presence of interleukin-6 (IL-6), retinoic acid (RA) or dexamethasone (DX), and some plasma proteins secreted to the media were determined by electroimmunoassay whereas the contents of specific mRNAs in the cells was evaluated by Northern blot hybridization. Interleukin-6 maximally stimulated synthesis of alpha-1-antichymotrypsin between days 1 and 3 whereas the response of fibrinogen was delayed to days 3 to 7. Retinoic acid increased the effect of IL-6 on alpha-1-antichymotrypsin (ACT) and fibrinogen (FBG) on the level of both proteins and mRNAs. Synthesis of albumin was slightly inhibited by IL-6 and RA, and synthesis of transferrin was increased by RA but not by IL-6. Dexamethasone had small enhancing effect on the action of IL-6. These results suggest that long-term HepG2 cultures may provide an experimental model for liver acute phase response during chronic inflammation.

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