Identification of an arthropod molecular target for plant-derived natural repellents

Tian, Quan; Wang, Peiyu; Xie, Chang; Pang, Peiyuan; Zhang, Youjing; Gao, Yue; Cao, Zhijian; Wu, Yingliang; Li, Wenxin; Zhu, Michael X.; Li, Dongdong; Yao, Jing

doi:10.1073/pnas.2118152119

Cited by 7 publications

(2 citation statements)

References 42 publications

(47 reference statements)

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“…We describe the antennae-expressed TcTRPA1-mediated repellency behavior by which T. castaneum responds to citronellal, our results suggest that coleopteran TRPA1 represents a potential target for sustainable insect repellents. Intriguingly, Tian et al recently reported that D. melanogaster TRPγ, contained in TRPC subfamily, was directly activated by citronellal to initiate repellent behavior 38 , for which further experiments and screening are desirable even in coleopteran beetles.…”

Section: Discussionmentioning

confidence: 99%

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Shimomura

Ino

Tamura

et al. 2022

Sci Rep

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The sensory perception of irritant chemicals results in escape and repellency behavioral patterns in insects. Transient receptor potential channels are cation channels that function as sensor proteins for several types of signals, such as light, sound, temperature, taste, as well as chemical and physical stimuli; among these, the TRPA channel is widely conserved and activated by irritant chemicals. Certain plant-derived essential oils (EOs), produced by secondary metabolism, are mixtures of volatile compounds, which are used as repellents because they contain environmentally sustainable ingredients. Citronellal, which is present in citronella EO from Cymbopogon species, is a potentially viable insect repellent; however, the repellency capability against coleopteran beetles remains limited. We investigated the citronellal-derived repellency behavior for the red flour beetle Tribolium castaneum, in which TcTRPA1 and odorant receptor co-receptor (Orco) expressions were mediated by RNA interference. Area-preference tests showed dose-dependent repellency behavior for citronellal; additionally, both TcTRPA1 and TcOrco double-strand RNA (dsRNA) micro-injection showed clear TcTRPA1 and TcOrco transcript reductions, and only TcTRPA1 dsRNA treatment significantly impaired repellency behavior. The relative expression level of the TcTRPA1 transcripts, evaluated by quantitative reverse-transcription PCR (qRT-PCR), revealed dominant expression in the antennae, indicating the antennae-expressed TcTRPA1-mediated repellency behavior.

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Section: Discussionmentioning

confidence: 99%

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Shimomura

Ino

Tamura

et al. 2022

Sci Rep

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“…[4] These phytochemicals rose to prominence is due to they are readily biodegradable, relatively less toxic, and less DOI: 10.1002/masy.202300005 harmful to the environment and the consumer. [5,6] A biologically active compound must have a correct hydrophilic and hydrophobic properties for optimum activity. [7] The best active lead molecules are usually too lipophilic nor hydrophilic.…”

Section: Introductionmentioning

confidence: 99%

Microwave‐Mediated Modification of Selected Limonoids with DMAB Reagent and Antibacterial Studies

Jayakumar,

Saxena,

Tomar

2024

Macromolecular Symposia

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N,N‐Dimethylaniline.borane (DMAB) is a stable, multifaceted electrophilic boron agent that displays hydroboration of C = C as well as selective reduction of functional groups under reflux, microwave, and ultrasound conditions. Natural products contain a plethora of functional groups that govern the specific bioactivity. It will be interesting to correlate the change in a functional group with the concern for change in bioactivity. Current research emphasizes the influence of microwave irradiation on moderately reactive DMAB reagent towards the functional group modification of selected limonoid‐phytochemicals of Meliaceae family viz. cedrelone, azadirone, nimbin, and azadiradione. DMAB conveniently reduces the ketone, epoxide group into alcohol, and does a hydroboration reaction on the C = C bond. It does not show interest to reduce the ester group irrespective of 30 min of MW irradiation. Except for these reducing properties, the reagent DMAB unveils chemo‐selective nature in respect of different functional groups and utilizes in an asymmetric synthesis. The coalescence of amine.borane with microwave irradiation brings forth a commodious and prompt method for the selective reduction of functional groups. The bioactive efficacy of modified phytochemicals is further taken for the antimicrobial studies.

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Identification of an arthropod molecular target for plant-derived natural repellents

Tian

Wang

Xie

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Significance Rational control of arthropod pests is important for animal and human health as well as biodiversity preservation. As an alternative to synthetic chemical pesticides, natural repellents represent an ecological method of pest control. Through an exceptional gene library screening in Mesobuthus martensii scorpions, we here uncover a transient receptor potential ion channel as the chemosensory sensor for plant-derived repellents. Its ortholog ion channel in Drosophila melanogaster also acts as a molecular receptor of natural repellents and mediates avoidance behavior. This work thus identifies a molecular basis for arthropod chemosensing and should help update the ecological strategies for pest control while preserving biodiversity.

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Identification of an arthropod molecular target for plant-derived natural repellents

Cited by 7 publications

References 42 publications

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Microwave‐Mediated Modification of Selected Limonoids with DMAB Reagent and Antibacterial Studies

Identification of an arthropod molecular target for plant-derived natural repellents

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