Identification of amino acid transport systems stimulated by FSH in rat testes

Curte, Anamaria; Wassermann, G. F.

doi:10.1677/joe.0.1060291

Cited by 17 publications

(4 citation statements)

References 6 publications

(10 reference statements)

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“…This FSH stimulatory action was independent of its effect on AC-cAMP activity and was observed using FSH from different species and sources (NHPP: ovine, porcine, and rat; Sigma-Aldrich: ovine) (Irusta and Wassermann, 1974; Pérez-Sánches and Wassermann, 1981; Cruz Curte and Wassermann, 1985; Spritzer and Wassermann, 1985; Silva and Wassermann, 1999; Silva et al, 2002). …”

Section: Discussionmentioning

confidence: 85%

Pertussis toxin nullifies the depolarization of the membrane potential and the stimulation of the rapid phase of 45Ca2+ entry through L-type calcium channels that are produced by follicle stimulating hormone in 10- to 12-day-old rat Sertoli cells

2010

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The aim of this study was to evaluate the effect of pertussis toxin (PTX) on the depolarizing component of the action of follicle stimulating hormone (FSH) on the membrane potential (MP) of Sertoli cells, which is linked to the rapid entry of Ca2+ into cells and to the Ca2+-dependent transport of neutral amino acids by the A system. This model allowed us to analyze the involvement of Gi proteins in the action of FSH in these phenomena. In parallel, using an inactive analog of insulin-like growth factor type I (IGF-1), JB1, and an anti-IGF-I antibody we investigated the possible mediating role of IGF-I on these effects of FSH because IGF-I is produced and released by testicular cells in response to stimulation by FSH and shows depolarization effects on MP similar to those from FSH. Our results have the following implications: (a) the rapid membrane actions of FSH, which occur in a time-frame of seconds to minutes and include the depolarization of the MP, and stimulation of 45Ca2+ uptake and [14C]-methyl aminoisobutyric acid ([14C]-MeAIB) transport, are nullified by the action of PTX and, therefore, are probably mediated by GiPCR activation; (b) the effects of FSH were also nullified by verapamil, an L-type voltage-dependent Ca2+ channel blocker; (c) wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K), prevented FSH stimulation of 45Ca2+ entry and [14C]-MeAIB transport; and (d) these FSH actions are independent of the IGF-I effects. In conclusion, these results strongly suggest that the rapid action of FSH on L-type Ca2+ channel activity in Sertoli cells from 10- to 12-day-old rats is mediated by the Gi/βγ/PI3Kγ pathway, independent of the effects of IGF-I.

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Section: Discussionmentioning

confidence: 85%

Pertussis toxin nullifies the depolarization of the membrane potential and the stimulation of the rapid phase of 45Ca2+ entry through L-type calcium channels that are produced by follicle stimulating hormone in 10- to 12-day-old rat Sertoli cells

2010

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“…Following the maximum hormonal effect, hyperpolarization remained in a steady state until the end of the measurement (35-45 s). For the experimental data, the dual effect of FSH on hyperpolarization, followed by depolarization, was found to be concentration-dependent as depolarization was induced by the Na+-amino acid co-transporter and involved calcium influx 12,29,31 . The model was implemented in MATLAB®, using the ode45 function to solve the differential equations running in Windows 7.…”

Section: Table 1 Values Used Inmentioning

confidence: 98%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques

Gamba

Menegaz

et al. 2022

Preprint

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses. The responses of Sertoli cells in culture to hormone stimulation were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools. A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K + pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells collected from intact seminiferous tubules and of Sertoli cells in culture. The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K + pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells. Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of non-excitable cells to hormonal stimuli.

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“…Following the maximum hormonal effect, hyperpolarization remained in a steady state until the end of the measurement (35-45 s). For the pharmacological experimental data, the dual effect of FSH on hyperpolarization, followed by depolarization, was found to be concentration-dependent as depolarization was induced by the Na + -amino acid co-transporter and involved calcium influx [12,30,33].…”

Section: Atp-dependent Na + /K + Pump Currentmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques,

Gamba,

Menegaz

et al. 2023

Biomed. Phys. Eng. Express

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses by involving ionic channels.The rapid responses of Sertoli cells in culture were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools (from intact seminiferous tubules). A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K+ pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells in the intact seminiferous tubules and of Sertoli cells in culture (patch clamp measurements).The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K+ pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells.Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of Sertoli cells to hormonal stimuli.

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Identification of amino acid transport systems stimulated by FSH in rat testes

Cited by 17 publications

References 6 publications

Pertussis toxin nullifies the depolarization of the membrane potential and the stimulation of the rapid phase of 45Ca2+ entry through L-type calcium channels that are produced by follicle stimulating hormone in 10- to 12-day-old rat Sertoli cells

Pertussis toxin nullifies the depolarization of the membrane potential and the stimulation of the rapid phase of 45Ca2+ entry through L-type calcium channels that are produced by follicle stimulating hormone in 10- to 12-day-old rat Sertoli cells

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

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