Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy

Li, Yingming; Sun, Su-Qin; Zhou, Qun; Zhu, Qi; Jiang, Tao; Wang, Jiang; Fang, Xiang

doi:10.1016/j.vibspec.2003.12.009

Cited by 80 publications

(36 citation statements)

References 6 publications

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“…25 Based on literature review, the FTIR spectrum for pure ginseng extract powder shows characteristic peaks at 2923 cm 21 due Ó 2015 Biomedical Engineering Society to stretching vibration of -CH 2 -groups and at 1633 cm 21 due to the stretching vibration of carbonyl group. 18 Peaks at 1056-1026 cm 21 are characteristic peaks to distinguish different types of Ginseng (from Korea, America or china). 18 PCL nanofibers have an extremely hydrophobic nature which is undesirable for tissue engineering applications especially as a bone construct.…”

Section: Physical and Mechanical Characteristics Of Scaffoldsmentioning

confidence: 99%

“…18 Peaks at 1056-1026 cm 21 are characteristic peaks to distinguish different types of Ginseng (from Korea, America or china). 18 PCL nanofibers have an extremely hydrophobic nature which is undesirable for tissue engineering applications especially as a bone construct. The hydrophobicity of pristine PCL nanofibers is the major reason for a slow degradation rate and a weak cell adhesion and proliferation on the surface of the fibers.…”

Section: Physical and Mechanical Characteristics Of Scaffoldsmentioning

confidence: 99%

“…28 The change in Runx-2 gene expression level among samples directly relates to the observed change of OCN expression, since Runx-2 can directly stimulate transcription of OCN-the most abundant nano collagenous protein in bone ECM. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] The up-regulation of OCN during matrix synthesis and mineralization, 27 is curtail for later stages of osteogenesis and ECM mineral formation. The obtained gene expression profile for MSCs seeded on PCL group was in agreement with other studies.…”

Section: Mscs Proliferation and Differentiation On Electrospun Scaffoldsmentioning

confidence: 99%

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Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

2015

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Medicinal plants as a therapeutic agent with osteogenic properties can enhance fracture-healing process. In this study, the osteo-inductive potential of Asian Panax Ginseng root extract within electrospun polycaprolactone (PCL) based nanofibers has been investigated. Scanning electron microscopy images revealed that all nanofibers were highly porous and beadles with average diameter ranging from 250 to 650 nm. The incorporation of ginseng extract improved the physical characteristics (i.e., hydrophilicity) of PCL nanofibers, as well as the mechanical properties. Although ginseng extract increased the degradation rate of pure PCL nanofibers, the porous structure and morphology of fibers did not change significantly after 42 days. It was found that nanofibrous scaffolds containing ginseng extract had higher proliferation (up to ~1.5 fold) compared to the pristine PCL. The qRT-PCR analysis demonstrated the addition of ginseng extract into PCL nanofibers induced significant expression of osteogenic genes (Osteocalcin, Runx-2 and Col-1) in MSCs in a concentration dependent manner. Moreover, higher calcium content, alkaline phosphatase activity and higher mineralization of MSCs were observed compared to the pristine PCL fibers. Our results indicated the promising potential of ginseng extract as an additive to enhance osteo-inductivity, mechanical and physical properties of PCL nanofibers for bone tissue engineering application.

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Section: Physical and Mechanical Characteristics Of Scaffoldsmentioning

confidence: 99%

Section: Physical and Mechanical Characteristics Of Scaffoldsmentioning

confidence: 99%

Section: Mscs Proliferation and Differentiation On Electrospun Scaffoldsmentioning

confidence: 99%

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Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

2015

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“…These were confirmed by infrared absorption bands (Fig 7-8 (Nakanishi, et al, 1977). The stronger the relative intensity of the absorption bands between 1150 and 700 cm -1 the higher the starch content (Nakanishi, et al, 1977;Li, et al, 2004;Hua, et al, 2003;Woo, et al, 1999). The difference in sugar chain of fraction 59-2 and 59-4 could therefore be explained by the fact that the former fraction eluted earlier than the latter due to stearic reasons.…”

Section: Ft-ir Spectra Of Saponins From E Elephantinamentioning

confidence: 99%

Cytotoxicity, phytochemical analysis and antioxidant activity of crude extracts from Rhizomes of <i>Elephantorrhiza Elephantina</i> and <i>Pentanisia Prunelloides</i>

Mpofu

Msagati

Krause

2013

Afr. J. Trad. Compl. Alt. Med.

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Background: Elephantorrhiza elephantina (Ee) and Pentanisia prunelloides (Pp) are two medicinal plants which are widely used to remedy various ailments including diarrhoea, dysentery, inflammation, fever, rheumatism, heartburn, tuberculosis, haemorrhoids, skin diseases, perforated peptic ulcers and sore joints in southern Africa (South Africa, Swaziland, Botswana and Zimbabwe). The following study was conducted to explore the in vitro cytotoxicity, antioxidant properties and phytochemical profile of the two medicinal plants. Materials and Methods:The cytotoxicity of the aqueous and methanol extracts and fractions of both species was studied using the brine shrimp lethality tests (BST) for the first time. Results:The results demonstrated that the lethality (LC 50 ) for crude extracts for both plants ranged between 1.8 and 5.8 ppm and was relatively greater than that for the methanol, ethyl acetate and chloroform fractions of the extracts which ranged between 2.1 ppm and 27 ppm. This suggested that crude extracts were more potent than their respective fractions, further explaining that the different fractions of phytochemicals in these plant species work jointly (in synergy) to exert their therapeutic efficacy. Both aqueous and methanol extracts of the two medicinal plants demonstrated a high degree of antioxidant capacity against the DPPH radical with the Duh and Yen inhibition percentage ranging between 4.5% and 72%. Phytochemical studies of the rhizome extracts showed that the major compounds present include flavonoids, tannins, anthocyanidins, anthraquinones, triterpenoids (oleanolic acid), the steroidal saponin Diosgenin, the sugars, rhamnose, glucuronic acid, Arabinose and hexoses. Conclusion: This is the first report of the detection and isolation of diosgenin and oleanolic acid from the rhizome extracts of Ee and Pp. All structures were determined using spectroscopic/spectrometric techniques (1H NMR and 13C and LC-ESI-MS) and by comparison with literature data.

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“…use to fingerprint herbal extracts or powders, is relatively uncommon compared with chromatographic and classical methods (Hussain et al, 2009a;Li et al, 2004b;Liu et al, 2006). The use of attenuated total reflectance (ATR) device evolved rapid FTIR measurements of liquids such as oils and plant extracts, allowing the identification and quantification of valuable plant biomarkers (Schultz and Baranska, 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparative Fingerprint and Extraction Yield of Medicinal Herb Phenolics with Hepatoprotective Potential, as Determined by UV-Vis and FT-MIR Spectroscopy

Zavoi

Fetea

Ranga

et al. 2011

Not Bot Hort Agrobot Cluj

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The present study was aimed to compare the polyphenolic composition of six medicinal herbs, from wild flora of Romania. The plants investigated, Cynara scolimus (artichoke), Taraxacum officinalis (dandelion), Chelidonium majus (celandine), Hypericum perforatum (St. John's wort), Silybum marianum (Mary thistle) and Lycopodium clavatum (Wolf 's claw) are known, to have hepatoprotective action. Using in parallel glycerol-water, ethanol-water and methanol, the solvent-dependence of the extract fingerprint and composition in bioactive molecules was studied by UV-Vis and Infrared (FT-MIR) spectrometry. The extraction yields, calculated as an extraction factor (EF) were superior in acidic methanol comparative to glycerin and ethanol, favorising the increase in phenolic acids against flavonoid derivatives . Based on the differences of polarity between the three solvents used, higher EF values were obtained for dandelion, artichoke, celandine and St. John wort, more rich in phenolic acids than flavonoids. Mary thistle and Wolf 's claw had lower concentrations of phenolics, but higher content of lignans and terpenoids. Based on the FT-MIR peaks from 8 regions, for each plant extract, has been determined the fingerprint region between 900 and 1500 cm -1 and identified the specific functional groups. A good, significant correlation was found between the concentration of total phenolics calculated by UV-Vis spectrometry and FTIR methods, after calibration with gallic acid. The value of the MIR signal at 1743 cm -1 may be considered a good indicator of phenolics concentration in such extracts. Combined UV-Vis and FTIR spectroscopy are recommended as rapid and reliable tools to investigate the fingerprint and to predict the composition of medicinal plants or to evaluate the quality and authenticity of different standardized formulas.

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Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy

Cited by 80 publications

References 6 publications

Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

Cytotoxicity, phytochemical analysis and antioxidant activity of crude extracts from Rhizomes of <i>Elephantorrhiza Elephantina</i> and <i>Pentanisia Prunelloides</i>

Comparative Fingerprint and Extraction Yield of Medicinal Herb Phenolics with Hepatoprotective Potential, as Determined by UV-Vis and FT-MIR Spectroscopy

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