Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)

Ebner, Christof; Hirschwehr, Reinhold; Bauer, Leonhardt; Breiteneder, Heimo; Valenta, Rudolf; Ebner, H; Kraft, Dietrich; Scheiner, Otto

doi:10.1016/s0091-6749(95)70096-x

Cited by 375 publications

(253 citation statements)

References 29 publications

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“…These findings agree with the current understanding of profilin as a plant panallergen (Valenta et al, 1992;Radauer et al, 2006;Ebner et al, 1995).…”

Section: Discussionsupporting

confidence: 91%

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Sensitization to profilin in the Central region of Portugal

Tavares

Machado

Loureiro

et al. 2008

Science of The Total Environment

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“…These findings agree with the current understanding of profilin as a plant panallergen (Valenta et al, 1992;Radauer et al, 2006;Ebner et al, 1995).…”

Section: Discussionsupporting

confidence: 91%

“…This can be explained by sensitization to panallergens present in pollens, namely profilins and calcium-binding proteins (Valenta et al, 1991(Valenta et al, , 1992(Valenta et al, , 1998Radauer et al, 2006;Ebner et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Sensitization to profilin in the Central region of Portugal

Tavares

Machado

Loureiro

et al. 2008

Science of The Total Environment

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“…The concentrations of Pru p 3 and synthetic peptides were determined by dose-response experiments using PBMCs from two different patients and 0-50 |jig/ml of Pru p 3 or each peptide. These concentrations were similar to those previously published for other allergens (Ebner et al, 1995;Zeiler et al, 1999;Jahn-Schmidetal., 2002). Phytohemagglutinin-L from Phaseolus vulgaris (PHA; 1 pg/ml; Roche, Mannheim, Germany) was used as a positive control to evaluate the proliferative response of PBMC cultures.…”

Section: Proliferation Assays With Pbmcssupporting

confidence: 91%

“…Additionally, Pru p 3 50 _6o was recognized by control subjects, but not by PBMCs or TCLs derived from patients. Potential T-cell epitopes of other allergens, such as Art v 1 and Bet v 1, have been reported to also stimulate T-cell from both patients and control subjects (Ebner et al, 1995;Haselden et al, 2001;JahnSchmid et al, 2002;Glaspole et al, 2005). This fact suggests that the switch to allergic tolerance must not operate in the antigen-T-cell recognition, but in a subsequent step.…”

Section: Discussionmentioning

confidence: 99%

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Tordesillas¹,

Cuesta‐Herranz

González‐Muñoz

et al. 2009

Molecular Immunology

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Keywords:Lipid transfer protein T-cell epitope Peptide immunotherapy Plant food allergy Peach Pru p3Lipid transfer proteins (LTPs), particularly peach Pru p 3, are the most relevant plant food allergens in the South of Europe, and, therefore, their allergic properties have been extensively studied. However, neither T-cell epitopes nor their effect on the patients' T-cell response has been investigated in any member of the LTP panallergen family. The objective of the present study was to map the major T-cell epitopes of Pru p 3, as well as to evaluate their induced T-cell response in peach-allergic versus control subjects. Thus, peripheral blood mononuclear cells (PBMCs) from 18 peach-allergic patients and Pru p 3-specific T-cell lines (TCLs) from 9 of them were cultured with Pru p 3 and with a panel of 17 derived peptides (10-mer overlapping in 5 amino acids representing the full sequence of Pru p 3). Proliferation in 5-day assays was carried out via tritiated-thymidine incorporation, while IL4 and IFN7 production was assessed via sandwich enzymelinked immunosorbent tests (ELISA) of TCL culture supernatants. The results were compared to those obtained from 10 non-peach allergic control volunteers. Two consecutive peptides showed the highest activation capacity. About 74% of PBMCs and TCLs recognized them, forming a single T-epitope: Pru p 365-80-Additionally, other specific T-cell epitopes were observed. Pru p 3 2 s-35 was detected by more than 60% of TCLs from peach-allergic patients, and Pru p 345-55 only activated PBMCs from control subjects. Interestingly, TCLs from patients were associated with a Th2-type, whereas control TCLs presented a Thltype cytokine response. The major immunogenic T-cell epitope identified in Pru p 3, Pru p 365-so, is a good candidate to develop new vaccines for hypersensitivity reactions associated with LTP allergens from Rosaceae fruits.

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“…Carrot allergy is generally considered a birch pollen‐related food allergy with Dau c 1 as major allergen 2. Birch pollen‐related food allergy results from primary sensitization to the major birch pollen allergen followed by cross‐reactivity of Bet v 1‐specific IgE antibodies and T cells with Bet v 1‐related food proteins 3, 4, 5, 6. Consequently, Bet v 1 and homologous food allergens are usually not considered to show sensitizing activity.…”

mentioning

confidence: 99%

Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

Zulehner

Nagl

Briza

et al. 2016

Allergy

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BackgroundIn contrast to other Bet v 1‐related food allergens, the major carrot allergen, Dau c 1, has been suggested to induce food allergy independently from Bet v 1. As T cells are crucial in the sensitization process, we sought to characterize the T‐cell response to Dau c 1 and its cross‐reactivity with Bet v 1.MethodsDau c 1‐specific T‐cell lines (TCL) and clones (TCC) established from PBMC of birch pollen‐allergic patients with carrot allergy were analyzed for reactivity to Bet v 1, epitope specificity, allergen‐induced cytokine secretion, and expression of integrins α4β7 and α4β1, critical for gut and lung homing, respectively. mRNA expression of GATA3 and Tbet was analyzed in sorted CD3+ CD4+ CFSE low cells proliferating upon stimulation of PBMC with Dau c 1 or Bet v 1. Dau c 1 was incubated with endolysosomal proteases, and the resulting fragments were identified by mass spectrometry.ResultsAmong 14 distinct T‐cell‐activating regions, Dau c 1139–153 was recognized by 55% of the patients. Only 6 of 15 (40%) Dau c 1‐specific TCL and 9 of 21 (43%) TCC reacted with Bet v 1. Bet v 1‐nonreactive TCC were mainly Th1‐like and showed a higher expression of the integrin β7 and a significantly lower expression of the integrin β1 than Bet v 1‐positive TCC. A Th1‐like response was also detected in Dau c 1‐reactive CD3+ CD4+ CFSE low cells. Full‐length Dau c 1 was still detectable after 48 h of endolysosomal degradation. Proteolytic fragments of Dau c 1 matched its T‐cell‐activating regions.ConclusionDau c 1 displays several characteristics of sensitizing allergens, namely a major T‐cell‐activating region, low susceptibility to endolysosomal degradation, and induction of a Bet v 1‐independent T‐cell response. These cellular insights confirm that the major carrot allergen has a special status among Bet v 1‐related food allergens.

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Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)

Cited by 375 publications

References 29 publications

Sensitization to profilin in the Central region of Portugal

Sensitization to profilin in the Central region of Portugal

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

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