Identification of allergens and antigens of Bermuda grass (Cynodon dactylon) pollen by immunoblot analysis

Abstract

Summary Allergens and antigens of Bermuda grass pollen fractionated by SDS‐polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes were identified using twenty‐one sera of Bermuda grass pollen‐allergic patients. The IgE‐ and IgG‐binding pollen components transferred to nitrocellulose were detected by reaction with enzyme‐labelled anti‐human IgE and anti‐human IgG, respectively. There was heterogeneity in both IgE‐ and IgG‐binding patterns of the allergic sera tested. Fourteen pollen components, ranging in molecular weight from 16000 to 88000 daltons, bound to IgE antibodies. Only two of the fourteen allergens identified reacted with IgE antibodies of more than 50% of the twenty‐one allergic sera. The pollen component with a molecular weight of 32000 daltons showed by far the highest frequency of IgE binding, being recognized by sixteen (76%) of the twenty‐one sera examined. Fifteen (71 %) of the twenty‐one sera tested had IgE antibodies that reacted with more than one of the fourteen allergenic components identified. Pollen components recognized by IgE antibodies also reacted with IgG antibodies, and there were components only recognized by IgG antibodies. Results obtained from this study should be useful both clinically and in research.

Connecting corporations and communities: Towards a theory of social inclusive open innovation

“…Myrtaceae Leaves Perennial Diarrhoea, dysentery (Nadkarni 1976), strengthening the teeth (Kirtikar and Basu 1999) Antihyperglycemic effect (Teixeira et al 1989), Antioxidant activity (Eshwarappa et al 2014), Antimicrobial, Antioxidant, Anticancer Activity (Kiruthiga et al 2011), Anti-inflammatory (Roy et al 2011), anti-allergic activity (Brito et al 2007) 18 Pui (Soraya et al 2015); anticancer activity (Kowsalya et al 2015); Antidiabetic activity (Jarald et al 2008); Diuretic Activity (Aruna et al 2013);Antiarthritic activity (Bhangale and Acharya 2014); anticonvulsive property (Odenigbo and Awachie 1993) (Shen et al 1988) (Subramanian and et al 1986) (Najafi et al 2009) (Najafi et al 2007), Antiulcer activity (Patil et al 2003a) 21 Koliyar Anti-Inflammatory Activity (Maity et al 1998) (Jain and Patil 2010), AntiProliferative Activity (Rejiya et al 2009), Anti-Microbial Activity (Bhatt...…”

Mapping of IgE and IgG₄ Antibody-Binding Epitopes in Cyn d 1, the Major Allergen of Bermuda Grass Pollen

“…Aeroallergens can induce type I hypersensitivity such as allergic rhinitis, conjunctivitis and asthma which are a great threat for quality of life and economic expenses [1]. Bermuda grass (Cynodon dactylon) pollen (BGP) is an important source of seasonal aeroallergens in tropical and subtropical areas worldwide [2,3,4,5,6]. In Taiwan, the positive rate of immediate skin tests to the crude BGP extract was about 27% in asthmatic patients [2].…”

“…Using immunoblotting, several major human IgE-reactive BGP allergens have been identified. Cyn d 1, the most abundant protein in BGP extracts, has been recognized as the major and group 1 allergen of BGP [3,4,5,6]. …”

Purification and Characterization of a Novel Isoallergen of a Major Bermuda Grass Pollen Allergen, Cyn d 1

“…It contains multiple IgE-binding proteins [7,14,24,27]; some of these proteins have been isolated and proven to be allergens [8,10,15,16,21,23,25,[27][28][29]. One of the IgE-binding proteins, Cyn d 1, a major allergen of Bermuda grass pollen, contains 11 isoforms whose pI values range from 5.6 to 7.4, with N-terminal amino acid sequences which show significant sequence homology [3].…”