Identification of AFLP markers linked to one recessive genic male sterility gene in oilseed rape, Brassica napus

Abstract

The commercial utilization of heterosis in seed yield by means of hybrid varieties is of great importance for increasing oilseed rape production in China. This requires a functional system for the production of hybrid seed. The Brassica napus oilseed rape line 9012AB is a recessive epistatic genic male sterility (GMS) two-type line, in which the sterility is controlled by two pairs of recessive duplicate sterile genes (ms1 and ms2) interacting with one pair of a recessive epistatic inhibitor gene (rf). Homozygosity at the rf locus (rfrf) inhibits the expression of the recessive male sterility trait in homozygous ms1ms1ms2ms2 plants. This study was conducted to identify molecular markers for one of the male fertility/sterility loci in the B. napus male sterility line 9012AB. Sterile bulk (BS) and fertile bulk (BF) DNA samples prepared from male sterile and male fertile plants of the homozygous two-type line 9012AB were subjected to amplified fragment length polymorphic (AFLP) analysis. A total of 256 primer combinations were used and seven markers tightly linked to one recessive genic male sterile gene (ms) were identified. Among them, six fragments co-segregated with the target gene in the tested population, and the other one had a genetic distance of 4.3 cM. The markers identified in this study will greatly enhance the utilization of recessive GMS for the production of hybrid seed in B. napus oilseed rape in China.

Exploiting comparative mapping among Brassica species to accelerate the physical delimitation of a genic male-sterile locus (BnRf) in Brassica napus

“…In turn, these populations can be used as female parents to be pollinated by restorer lines, giving rise to fertile three-way hybrids. This approach for hybrid production greatly overcomes the inevitable restriction of previously reported RGMS lines, such as S45AB and 117AB, in which 50% of the fertile plants would be artificially removed from the female lines before flowering (Chen et al 1998;Ke et al 2005). Thus, the application of 9012AB in hybrid production was widely promoted in China, and a series of commercial cultivars were released .…”

“…The RGMS two-type line 9012AB and the temporary maintainer T45 used in our study have been described by Ke et al (2005) and Xie et al (2008), respectively. According to the recently modified hereditary pattern of this RGMS line, the genotypes of 9012A, 9012B and T45 are Bnms3ms3Rf b BnRf b , BnMs3ms3Rf b Rf b and Bnms3ms3Rf c Rf c , respectively (Dong et al 2010).…”

“…Second, attempts toward final isolation of the genes responsible for male sterility in 9012AB have been carried out. For example, the genetic map of BnMs3 was gradually narrowed by continual efforts (Ke et al 2005;Huang et al 2007;He et al 2008). Now, the BnMs3 locus has been cloned and characterized as a member of the protein import complex responsible for the transport of nuclear-encoded proteins to the chloroplast stroma (Dun et al 2011).…”

Map-based cloning of a recessive genic male sterility locus in Brassica napus L. and development of its functional marker

“…Besides these systems, a recessive genic male sterility (RGMS or recessive GMS) line 9012AB (Chen et al 1993) has also been increasingly adopted in China, due to the remarkable advantages including the capability of generating a complete male-sterile population and almost no restriction of restorer (Ke et al 2005;He et al 2008). Initially, the male sterility in 9012AB was regarded to be conditioned by that the interaction of three individual loci (BnMs3, BnMs4 and BnRf) (Chen et al 1998).…”

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

“…Miao et al (2003) identified four sequence-tagged site (STS) markers that are tightly linked to a recessive male-sterile gene in Chinese cabbage; A group of homologs of the male-sterility or fertility gene have been isolated, and the relevant biological functions have been revealed , Cao et al 2006, Jiang and Cao 2007, Tian et al 2009Zhang et al (2008) identified a random amplified polymorphic DNA (RAPD) marker that is tightly linked to a dominant male sterile gene and converted it into a sequence characterized amplified region (SCAR) marker. Wang et al (2000), Ke et al (2005), Yi et al (2006), Hong et al (2006), Lei et al (2007) and Huang et al (2007) identified a set of molecular markers that are linked to the male sterile gene in Brassica napus. Wang et al (2000aWang et al ( , 2000b identified 12 amplified fragment length polymorphism (AFLP) markers, a SCAR marker and an extended random primer amplified region (ERPAR) marker that are closely linked to a dominant male-sterile gene in Brassica oleracea, and mapped the gene to linkage group 09, corresponding to chromosome 3 of B. oleracea.…”