Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers

Xiao, Jian; Chen, Ligeng; Xie, Min; Liu, Hailin; WeiQi, Ye

doi:10.1016/j.scienta.2009.03.006

Cited by 28 publications

(14 citation statements)

References 22 publications

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“…This is the case of the monoembryonic cultivar “Fortune,” widely used as female parent in the triploid breeding programs in Spain (Navarro et al, 2012), France and Italy (Recupero et al, 2005; Froelicher et al, 2012); the susceptible cultivars “Murcott” and “Ponkan” are widely used as male parents in many diploid breeding programs carried out in Japan and Brazil (JinPing et al, 2009; Schinor et al, 2012). Also, this is the case for “Dancy,” “Minneola,” “Nova,” “Fairchild,” “Fremont,” “Page,” “Orlando,” “Pixie,” and “Daisy,” which are also used as male parents or for induced mutations in diploid and triploid breeding programs in Spain (Navarro et al, 2012) and USA (Williams, 2012).…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level.

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Section: Introductionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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“…However, adoption of this fruit tree in the market remains limited, although its popularity could be expanded by developing varieties that are more attractive to consumers (Manica 2003). In particular, one characteristic that is of great interest is aspermia, which has been reported for some naturally occurring mutants of A. squamosa (Araújo and Alves 1999, Manica 2003, Lora et al 2011 and has shown economic significance in several species (Varoquaux et al 2000, JinPing et al 2009, Zhang et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Stenospermy and seed development in the “Brazilian seedless” variety of sugar apple (Annona squamosa)

Santos

Ribeiro

Mercadante-Simões

et al. 2014

An. Acad. Bras. Ciênc.

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Stenospermy was identified in naturally occurring sugar-apple (Annona squamosa) mutants with great potential for use in genetic improvement programs. However, to date, there have been no detailed studies of the development of aspermic fruit in this species. The aim of the present study was to characterize the anatomy of developing fruit in the 'Brazilian Seedless' mutant. Flower buds in pre-anthesis and developing fruits were subjected to common plant anatomy techniques. The abnormal ovules are unitegmic and orthotropic and have a long funiculus. There is evidence of fertilization, including the presence of embryos in early development and the proliferation of starch grains in the embryo sac. However, the embryos and embryo sac degenerate, although this does not affect pericarp development. Ovule abortion does not occur. The perisperm, which is formed from the peripheral layers of the nucellus, fills the cavity left by the embryo sac. The mature fruit contains numerous small sterile seeds with abundant perisperm and unlignified integument that is restricted to the micropylar region. The majority of perisperm cells are living and appear to be metabolically active in the periphery. Therefore, stenospermy leads to the formation of sterile seeds in A. squamosa, and the perisperm possibly play an important role in fruit development.

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“…The methodology of pooling DNA for isolation of trait-specific markers has already been reported in other plants like Chinese jujube (Peng et al 2001), Ponkan mandarin (Jin Ping et al 2009) and betel vine (Khadke et al 2012). The RAPD technique is one of the most widely used and its popularity derives from its simplicity and rapidity, the requirement for only a small quantity of DNA, and the ability to generate numerous polymorphisms.…”

Section: Resultsmentioning

confidence: 99%

Application of RAPD markers in hybrid verification in coconut

Rajesh

Preethi

et al. 2014

Crop Breed. Appl. Biotechnol.

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Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers

Cited by 28 publications

References 22 publications

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Stenospermy and seed development in the “Brazilian seedless” variety of sugar apple (Annona squamosa)

Application of RAPD markers in hybrid verification in coconut

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