Application of RAPD markers in hybrid verification in coconut

Abstract

-Coconut palms are classified into two major types, viz., 'talls' and 'dwarfs', which

Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers

“…The present study revealed that RAPD was a valuable tool for cultivar identification. Our results are in agreement with previous research on coconut (Rajesh et al 2014), peach (Han et al 2014), durian (Rosmaina et al 2016) and cashew (Bhadra et al 2019). This is the first report on utilizing of DNA marker to identify Matoa (Pometia pinnata).…”

“…The identification and understanding of the relationship among plant cultivars using the DNA molecular approach are more effective than morphological markers because DNA analysis allows direct access to the hereditary material, not influenced by the environment, independent from the season, and no limit of plant stage (Nadeem et al 2018;Mirzaei 2021). RAPD markers have been known as one of the most commonly used techniques for cultivar identification, for example, apple (Antic et al 2016), Cherry (Atnafu 2016), durian (Rosmaina et al 2016), cashew (Bhadra et al 2019), citrus (El-Khayat 2019), hybrid purity identification (Rajesh et al 2014;Mylonas et al 2014;Pujar et al 2017), estimating the genetic diversity of plants and the population genetic structure of Panax ginseng (Wang et al 2016), Dalbergia sissoo (Dobhal et al 2019), Eurycoma apiculata (Zulfahmi et al 2021) due to their advantages compared to other DNA molecular techniques such as its simplicity, fast, cost-effective, short primers of arbitrary sequences, require small amounts of target DNA as well as no need of prior sequence information on the target species and radioactivity free procedure (Williams et al 1990;Nadeem et al 2018;Amiteye 2021). On the other hand, RAPD has some weaknesses, such as low reproducibility and dominant properties (heterozygotes and homozygotes can not be differentiated) (Kumari & Thakur 2014).…”

Development of a Molecular Marker for the Resistance Gene R11 of Potato to Late Blight

“…However, RAPD markers have two major disadvantages: they have low reproducibility and generally are not transferable (Bardakci, 2001;Collard et al, 2005). But it is used for genotyping and molecular breeding for several species (Pop et al, 2010;Alam, et al, 2014;Rajesh et al, 2014;Mhuka et al, 2016Mhuka et al, /2017Olawuyi and Azeez, 2019), and still found useful (Costa et al, 2016). The transformation of RAPD markers to Sequence characterized amplified region (SCAR) markers is an option to overcome these disadvantages.…”