Identification of a Unique Helicobacter Species by 16S rRNA Gene Analysis in an Abdominal Abscess from a Patient with X-Linked Hypogammaglobulinemia

Abstract

A unique Helicobacter species, MZ640285, was isolated from a patient with X-linked hypogammaglobulinemia suffering from recurrent abdominal abscesses and was identified by 16S rRNA gene sequence analysis. In the phylogenetic tree, the isolate fell into a cluster which included Flexispira rappini,Helicobacter bilis, and Helicobacter sp. strain Mainz. Helicobacters are being increasingly recognized as pathogens in immunocompromised hosts. These fastidious bacteria are not easily cultured in the routine diagnostic laboratory, and this is the first report of their identification by 16S rRNA gene sequencing performed directly from a clinical specimen.

A novel compound from celery seed with a bactericidal effect against Helicobacter pylori

“…H. pylori strain 26695 was obtained from Professor David Kelly (University of Sheffield) 24 . The identity of the strain was confirmed by Gram stain, urease reaction and the unique 16S rRNA gene sequence 1,25 . The bacteria were stored at −80°C in 1‐ml amounts of Brucella broth containing 15% (v/v) glycerol 26 .…”

Pyoderma Gangrenosum–Like Ulcer in a Patient With X-Linked Agammaglobulinemia

“…Bacterial DNA was directly extracted from the pus, and 16S ribosomal RNA gene sequence analysis identified a unique Helicobacter species closely related to F rappini and H bilis. 8 By 16S ribosomal RNA sequencing of bacteria from blood cultures, F rappini was identified as the organism that caused fevers and cellulitis of the legs in another patient with XLA. 9 As originally described by Brunsting et al, 10 typical PG manifests as rapidly progressive, painful, suppurative ulcers with undermined, ragged, purplish borders.…”

A novel compound from celery seed with a bactericidal effect against <I>Helicobacter pylori</I>

“…[24] The identity of the strain was confirmed by Gram stain, urease reaction and the unique 16S rRNA gene sequence. [1,25] The bacteria were stored at -80 o C in 1-ml amounts of Brucella broth containing 15% (v/v) glycerol. [26] H. pylori was cultured at 37 C under microaerobic conditions (10% CO 2 , 3% O 2 and 87% N 2 ) on chocolate agar plates or in liquid Brucella broth (BBL, Franklin Lakes, NJ, USA) supplemented with foetal bovine serum (5% v/v; Invitrogen, Paisley, UK) immediately before use.…”