Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in Atlantic salmon during Aeromonas salmonicida infection

Tsoi, Stephen; Park, Kyoung C.; Kay, Helen H.; O’Brien, Timothy J.; Podor, Eszter; Sun, Genlou; Douglas, Susan E.; Brown, Laura L.; Johnson, Stewart C.

doi:10.1016/j.vetimm.2005.05.013

Cited by 57 publications

(25 citation statements)

References 11 publications

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“…The time-course expression levels of TLR5S during A. salmonicida challenge have been described previously [29,30]. In the current study, there was enhanced expression of TLR5M in lymphoid organs of the resistant family, and lower levels in lymphoid organs of the susceptible family, which may be consistent with the cumulative mortality differences between the two families.…”

Section: Discussionsupporting

confidence: 89%

“…An EST sequence (DY712024) of Atlantic salmon has been submitted in the Genbank, and its aa identity with rainbow trout TLR5M (AB091105), TLR5S (AB062504), and Atlantic salmon TLR5S (AY628755) clearly proposed that the present EST belonged to Atlantic salmon TLR5M (data not shown). It has been suggested by studies that some pathogen-derived factors other than flagellin may contribute to the activation of TLR5 [29].…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Zhang¹,

Niu²,

Storset

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Zhang¹,

Niu²,

Storset

et al. 2011

Fish & Shellfish Immunology

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“…Knowing that flagellin is a pathogen-associated molecular pattern that directly interacts with TLR5, and considering the descriptions in Salmo salar of TLR5S and TLR5M (Tsujita et al 2004, Tsoi et al 2006, the impact of P. salmonis flagellin on TLR5 expression was evaluated. Specifically, a lab-produced recombinant His-tagged FlaA protein was used to stimulate SHK-1 cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of flagellin and key regulatory flagellar genes in the non-motile bacterium Piscirickettsia salmonis

Carril

Gómez²,

Marshall³

2017

Dis. Aquat. Org.

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The Piscirickettsia salmonis genome was screened to evaluate potential flagellarelated open reading frames, as well as their genomic organization and eventual expression. A complete and organized set of flagellar genes was found for P. salmonis, although no structural flagellum has ever been reported for this bacterium. To gain further understanding, the hierarchical flagellar cascade described for Legionella pneumophila was used as a reference model for putative analysis in P. salmonis. Specifically, 5 of the most relevant genes from this cascade were chosen, including 3 regulatory genes (fleQ, triggers the cascade; fliA, regulates the σ28-coding gene; and rpoN, an RNA polymerase-dependent gene) and 2 terminal structural genes (flaA and flaB, flagellin and a flagellin-like protein, respectively). Kinetic experiments evaluated gene expressions over time, with P. salmonis assessed in 2 liquid, cell-free media and during infection of the SHK-1 fish cell line. Under all conditions, the 5 target genes were primarily expressed during early growth/infection and were differentially expressed when bacteria encountered environmental stress (i.e. a high-salt concentration). Intriguingly, the flagellin monomer was fully expressed under all growth conditions and was located near the bacterial membrane. While no structural flagellum was detected under any condition, the recombinant flagellin monomer induced a proinflammatory response in SHK-1 cells, suggesting a possible immunomodulatory function. The potential implications of these observations are discussed in the context of P. salmonis biology and pathogenic potential.

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“…However, Tlr5 gene expression in the control group was significantly higher after infection. Although L. garvieae is not a flagellated bacterium, and Tlr5 is involved in the recognition of flagellin, previous studies have suggested that some pathogenderived factors other than flagellin may contribute to its activation [32,33].…”

Section: Discussionmentioning

confidence: 99%

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

Pérez‐Sánchez

Balcázar

Merrifield

et al. 2011

Fish & Shellfish Immunology

204

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Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in Atlantic salmon during Aeromonas salmonicida infection

Cited by 57 publications

References 11 publications

Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Expression of flagellin and key regulatory flagellar genes in the non-motile bacterium Piscirickettsia salmonis

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

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