Identification of a sperm cell attribute responsible for subfertility of roosters homozygous for the rose comb allele

McLean, Derek J.; Froman, D. P.

doi:10.1095/biolreprod54.1.168

Cited by 16 publications

(11 citation statements)

References 18 publications

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“…Variation in the expression of the DLX gene repertoire has been linked to craniofacial dysmorphology in several species, most recently in cichlid fish (Renz et al, 2011). Subfertility has been reported in birds homozygous for rose comb phenotype (McLean and Froman, 1996). Givens et al (2005) found evidence to support the role of DLX1 in regulating the GnRH promoter during development in mice.…”

Section: Mendelian Traits In Traditional and Village Chickens D Wraggmentioning

confidence: 99%

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

et al. 2012

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Extensive phenotypic variation is a common feature among village chickens found throughout much of the developing world, and in traditional chicken breeds that have been artificially selected for traits such as plumage variety. We present here an assessment of traditional and village chicken populations, for fine mapping of Mendelian traits using genome-wide singlenucleotide polymorphism (SNP) genotyping while providing information on their genetic structure and diversity. Bayesian clustering analysis reveals two main genetic backgrounds in traditional breeds, Kenyan, Ethiopian and Chilean village chickens. Analysis of linkage disequilibrium (LD) reveals useful LD (r 2 X0.3) in both traditional and village chickens at pairwise marker distances of B10 Kb; while haplotype block analysis indicates a median block size of 11-12 Kb. Association mapping yielded refined mapping intervals for duplex comb (Gga 2:38.55-38.89 Mb) and rose comb (Gga 7:18.41-22.09 Mb) phenotypes in traditional breeds. Combined mapping information from traditional breeds and Chilean village chicken allows the oocyan phenotype to be fine mapped to two small regions (Gga 1:67.25-67.28 Mb, Gga 1:67.28-67.32 Mb) totalling B75 Kb. Mapping the unmapped earlobe pigmentation phenotype supports previous findings that the trait is sex-linked and polygenic. A critical assessment of the number of SNPs required to map simple traits indicate that between 90 and 110K SNPs are required for full genome-wide analysis of haplotype block structure/ancestry, and for association mapping in both traditional and village chickens. Our results demonstrate the importance and uniqueness of phenotypic diversity and genetic structure of traditional chicken breeds for fine-scale mapping of Mendelian traits in the species, with village chicken populations providing further opportunities to enhance mapping resolutions.

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Section: Mendelian Traits In Traditional and Village Chickens D Wraggmentioning

confidence: 99%

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

et al. 2012

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“…Likewise, no difference in fertility was observed following intramagnal insemination of testicular sperm from R/R males [4]. McLean and Froman [8] reported that sperm ejaculated by R/R males do not enter the SST after artificial insemination to the same extent as sperm from fertile controls. However, there is no genotypic difference in sperm penetration of SST when sperm are incubated with oviduct explants [8].…”

Section: Corvallis Oregon 97331mentioning

confidence: 97%

“…for the rose comb allele (R/R) are also subfertile [5,8]. Froman et al [9] demonstrated that these two traits have a cumulative effect.…”

Section: Corvallis Oregon 97331mentioning

confidence: 99%

“…Unlike sperm from Sd roosters, sperm from R/R roosters are viable and have normal morphology [10]. Behavior of sperm from R/R males within the hen's oviduct has been well characterized [4,8,11]. While intravaginal insemination of ejaculated sperm from R/R males resulted in subfertility [4,8], no difference in fertility was observed after intramagnal insemination of ejaculated sperm [4,11].…”

Section: Corvallis Oregon 97331mentioning

confidence: 99%

“…Behavior of sperm from R/R males within the hen's oviduct has been well characterized [4,8,11]. While intravaginal insemination of ejaculated sperm from R/R males resulted in subfertility [4,8], no difference in fertility was observed after intramagnal insemination of ejaculated sperm [4,11]. Likewise, no difference in fertility was observed following intramagnal insemination of testicular sperm from R/R males [4].…”

Section: Corvallis Oregon 97331mentioning

confidence: 99%

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Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean

Jones

Froman

1997

Biology of Reproduction

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Roosters homozygous for the rose comb allele (R/R) are subfertile. In previous research, these subfertile roosters were characterized by an in vitro sperm penetration assay as having limited sperm motility. The objectives in the present study were to characterize sperm motility by computer-assisted sperm motion analysis and to account for a mechanism underlying poor sperm motility. Percentages of motile sperm differed between subfertile males and fertile controls (r/r) by 29% (p < 0.001). The concentration of intracellular ATP in sperm form subfertile roosters was less than in that from fertile controls (p < 0.001). The genotypic difference is sperm motility, as measured with the sperm penetration assay, was maintained when ATP production was dependent on anaerobic glycolysis (p < 0.001). In this case, sperm were incubated with exogenous glucose and cyanide. Consequently, we could not attribute the genotypic difference in sperm mobility to mitochondrial respiration. In contrast, glucose transport, as measured by the uptake of [1,2-3H]-2-deoxy-D-glucose, was reduced in sperm from subfertile roosters (p < 0.001). Neither hexokinase nor glyceraldehyde-3-phosphate dehydrogenase activity differed between genotypes (p > 0.05). Likewise, lactate dehydrogenase activity did not differ between genotypes (p > 0.05). As evidenced by creatine kinase activity and dynein ATPase activity, neither the potential for energy transfer nor utilization within the axoneme differed between genotypes (p > 0.05). Therefore, we attribute the subfertility of roosters homozygous for the rose comb allele to decreased spermatozoal glucose transport.

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Semen Donor Selection by In Vitro Sperm Mobility Increases Fertility and Semen Storage in the Turkey Hen

DONOGHUE,

HOLSBERGER,

EVENSON

et al. 1998

Journal of Andrology

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Commercial turkey production relies on the artificial insemination (AI) of pooled semen. However, semen quality ultimately depends on the quality of individual ejaculates. The purpose of this study was to evaluate semen from individual toms by means of an objective sperm‐mobility assay. Semen was then pooled by mobility phenotype and inseminated into hens, and the percentages of fertile and hatched eggs were determined after egg incubation. To indirectly evaluate hens' sperm storage, we determined the number of sperm holes in the perivitelline layer (PL) of freshly laid eggs. Semen from individual ejaculates (two trials, total of 169 toms) was evaluated by use of the sperm‐mobility test (SMT). Semen was diluted to 1 × 109 sperm/ml, in prewarmed N‐tris‐[hydroxymethyl] methyl‐2‐amino‐ethanesulfonic acid (TES)‐buffered saline, and was placed over 3 ml of a 2% (w/v) Accudenz solution at 41° C. After a 5‐minute incubation period, the cuvette was placed in a densimeter, and percentage transmission was recorded after 1 minute. Semen samples from toms ranked, according to sperm mobility, in the highest 10% and the lowest 10%, after three evaluations, were pooled by group and were used to inseminate hens weekly (trial 1: n = 20 hens/group, for 10 weeks, AI dose = 150 × 106 spermatozoa inseminated fresh and after 24‐hour in vitro storage at 5° C; trial 2: n = 60 hens/group, for 16 weeks, AI dose = 75 × 106 spermatozoa inseminated fresh). Each week, eggs from day 6 post‐AI were evaluated for holes in the PL, vestiges of acrosomal induced hydrolysis. Spermatozoa from toms of different mobility phenotypes were also evaluated individually, for sperm chromatin structure and motility variables, by use of the Hobson Sperm Tracker. Toms characterized by high and low in vitro sperm‐mobility phenotype were categorized as “high mobility” and “low mobility,” respectively. The percentage of fertile eggs from hens inseminated with semen from the high‐mobility toms was higher than the percentage of fertile eggs from the low‐mobility group, in each trial (95.8 ± 1.3% vs. 90.4 ± 2.2%, and 88.7 ± 4.0% vs. 82.4 ± 0.4%, trials 1 and 2, respectively; P < 0.05). More sperm holes were observed in the PL of eggs fertilized by the high‐mobility toms than in the PL of eggs fertilized by the low‐mobility toms (P < 0.05). No differences in susceptibility of sperm nuclear DNA to denature in situ, as measured by the flow‐cytometric sperm chromatin‐structure assay, were detected between toms of differing mobility phenotypes. Sperm‐motility variables, straight‐line velocity, and average‐path velocity were significantly greater for high‐mobility toms compared to low‐mobility toms (P < 0.05). Sperm‐mobility differences between toms (detected by means of the SMT) influenced sperm storage, as indicated by the number of sperm in the PL and by the percentage of fertile eggs produced.

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Identification of a sperm cell attribute responsible for subfertility of roosters homozygous for the rose comb allele

Cited by 16 publications

References 18 publications

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

Semen Donor Selection by In Vitro Sperm Mobility Increases Fertility and Semen Storage in the Turkey Hen

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