Identification of a redox-dependent regulatory network of miRNAs and their targets in wheat

Cao, Jie; Gulyás, Zsolt; Kalapos, Balázs; Boldizsár, Ákos; Xin-ye, Liu; Pál, Magda; Yao, Yingyin; Galiba, Gábor; Kocsy, Gábor

doi:10.1093/jxb/ery339

Cited by 15 publications

(17 citation statements)

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“…The changes in the size of the glutathione pool (GSH+GSSG) and the GSSG/GSH ratio resulted in a daily oscillation in the half-cell reduction potential with an amplitude of about 20 mV (Supplementary Figure S3), which can greatly influence the function of redox-sensitive proteins. As observed earlier in wheat [64], a moderate increase could be detected in the GSH content during the light period, and high expression of HvAPSR in the meantime could contribute to this change by allowing a higher rate of Cys formation during the sulfate reduction and providing sufficient Cys for GSH synthesis. The low R/FR ratio increased both the expression of HvAPSR and Hvγ-ECS and the amount of Cys and GSH, indicating the spectral control of these genes and metabolites.…”

Section: Discussionsupporting

confidence: 70%

“…Thus, the GSSG accumulating during the light period can be regenerated by ascorbate peroxidase in the dark. High transcript levels of HvCAT2, HvCAT4 and HvPRX in cycling W light possibly cause a subsequent increase in the enzyme activity during the light period, as observed previously in wheat and Arabidopsis [30,64]. As described for HvGST and HvGR, maximum transcript levels of HvAPX1, HvAPX4, HvCAT2, HvCAT4 and HvPRX1 were also greater in low R/FR compared to W and supplemental B light during light/dark cycles, which further confirms the coordinated regulation of the various antioxidant genes by R and FR lights.…”

Section: Discussionsupporting

confidence: 59%

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Far-Red Light Coordinates the Diurnal Changes in the Transcripts Related to Nitrate Reduction, Glutathione Metabolism and Antioxidant Enzymes in Barley

Balogh

Kalapos

Ahres

et al. 2022

IJMS

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Spectral quality, intensity and period of light modify many regulatory and stress signaling pathways in plants. Both nitrate and sulfate assimilations must be synchronized with photosynthesis, which ensures energy and reductants for these pathways. However, photosynthesis is also a source of reactive oxygen species, whose levels are controlled by glutathione and other antioxidants. In this study, we investigated the effect of supplemental far-red (735 nm) and blue (450 nm) lights on the diurnal expression of the genes related to photoreceptors, the circadian clock, nitrate reduction, glutathione metabolism and various antioxidants in barley. The maximum expression of the investigated four photoreceptor and three clock-associated genes during the light period was followed by the peaking of the transcripts of the three redox-responsive transcription factors during the dark phase, while most of the nitrate and sulfate reduction, glutathione metabolism and antioxidant-enzyme-related genes exhibited high expression during light exposure in plants grown in light/dark cycles for two days. These oscillations changed or disappeared in constant white light during the subsequent two days. Supplemental far-red light induced the activation of most of the studied genes, while supplemental blue light did not affect or inhibited them during light/dark cycles. However, in constant light, several genes exhibited greater expression in blue light than in white and far-red lights. Based on a correlation analysis of the gene expression data, we propose a major role of far-red light in the coordinated transcriptional adjustment of nitrate reduction, glutathione metabolism and antioxidant enzymes to changes of the light spectrum.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 59%

Far-Red Light Coordinates the Diurnal Changes in the Transcripts Related to Nitrate Reduction, Glutathione Metabolism and Antioxidant Enzymes in Barley

Balogh

Kalapos

Ahres

et al. 2022

IJMS

Self Cite

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“…Cold stress [166] Wheat (Triticum aestivum) multiple miRNAs Reactive oxygen species (ROS) response [167] multiple miRNAs Water deficit and heat stress [168] TaemiR408…”

Section: Micrornas Stress Responses Referencementioning

confidence: 99%

MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops

Chaudhary

Grover

Sharma

2021

Life

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Crop yield is challenged every year worldwide by changing climatic conditions. The forecasted climatic scenario urgently demands stress-tolerant crop varieties to feed the ever-increasing global population. Molecular breeding and genetic engineering approaches have been frequently exploited for developing crops with desired agronomic traits. Recently, microRNAs (miRNAs) have emerged as powerful molecules, which potentially serve as expression markers during stress conditions. The miRNAs are small non-coding endogenous RNAs, usually 20–24 nucleotides long, which mediate post-transcriptional gene silencing and fine-tune the regulation of many abiotic- and biotic-stress responsive genes in plants. The miRNAs usually function by specifically pairing with the target mRNAs, inducing their cleavage or repressing their translation. This review focuses on the exploration of the functional role of miRNAs in regulating plant responses to abiotic and biotic stresses. Moreover, a methodology is also discussed to mine stress-responsive miRNAs from the enormous amount of transcriptome data available in the public domain generated using next-generation sequencing (NGS). Considering the functional role of miRNAs in mediating stress responses, these molecules may be explored as novel targets for engineering stress-tolerant crop varieties.

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“…miRNA are not coding RNA, which regulate gene expression at a post-transcriptional level in a suppressing mode [ 43 , 44 ]. The expression of miR395 is regulated under different abiotic stresses such as during sulfur starvation and drought [ 45 , 46 , 47 ]. Liang and co-workers demonstrated that miR395 modulates the accumulation of sulfate in rice by directly targeting the transcripts of ATPS and low affinity sulfate transporters [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

A Multifactorial Regulation of Glutathione Metabolism behind Salt Tolerance in Rice

et al. 2022

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Knowledge of the stress-induced metabolic alterations in tolerant and sensitive plants is pivotal for identifying interesting traits that improve plant resilience toward unfavorable environmental conditions. This represents a hot topic area of plant science, particularly for crops, due to its implication in food security. Two rice varieties showing dissimilar resistance to salt, Baldo and Vialone Nano, have been studied to investigate the mechanisms underpinning tolerance toward salinity, and these studies have focused on the root system. A detailed analysis of the salt stress-dependent modulation of the redox network is here presented. The different phenotype observed after salt exposure in the two rice varieties is coherent with a differential regulation of cell-cycle progression and cell-death patterns observed at root level. Baldo, the tolerant variety, already showed a highly responsive antioxidative capacity in control conditions. Consistently, stressed Baldo plants showed a different pattern of H2O2 accumulation compared to Vialone Nano. Moreover, glutathione metabolism was finely modulated at transcriptional, post-transcriptional, and post-translational levels in Baldo. These results contribute to highlight the role of ROS and antioxidative pathways as a part of a complex redox network activated in rice toward salt stress.

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Identification of a redox-dependent regulatory network of miRNAs and their targets in wheat

Abstract: A redox-dependent regulatory network of miRNAs and their targets were created using sequencing results, bioinformatics tools and correlation analysis of the examined biochemical and molecular parameters in wheat.

Cited by 15 publications

References 58 publications

Far-Red Light Coordinates the Diurnal Changes in the Transcripts Related to Nitrate Reduction, Glutathione Metabolism and Antioxidant Enzymes in Barley

Far-Red Light Coordinates the Diurnal Changes in the Transcripts Related to Nitrate Reduction, Glutathione Metabolism and Antioxidant Enzymes in Barley

MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops

A Multifactorial Regulation of Glutathione Metabolism behind Salt Tolerance in Rice

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