Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp

Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

“…In the control, the NKEF-B antiserum was replaced by the antiserum pre-adsorbed with pure fusion protein for 16 h at 4 C [31]. For Western blotting, the induced E. coli (expressing rNKEF-B) and un-induced E. coli were lysed in electrophoresis sample buffer.…”

Cloning, characterization and expression analysis of SIMP (source of immunodominant MHC-associated peptides) in grass carp Ctenopharyngodon idella

“…The control was designed to determine polyclonal antibody specificity. In the control, the gcSIMP antiserum was replaced by the antiserum preadsorbed with pure fusion protein for 16 h at 4 C [26]. For Western blotting, the gel-purified recombinant gcSIMP protein was lysed in an electrophoresis sample buffer.…”

Histone H2A Has a Novel Variant in Fish Oocytes1

“…Identification of oocyte-specific genes was recently initiated using subtractive hybridization in fish because of their diversity and particularities of reproduction modes [20,21]. Oocyte-specific genes, including zp3 [22,23], snx [24], clecdc (c-type lectin domain containing AY157616) [15], c1ql4l (AY662672) [16,25], spin1l (spindling 1-like AY627640) [26], and snrpc [27], were identified and characterized from Carassius auratus gibelio, a unique polyploid fish with dual reproduction modes of gynogenesis and sexual reproduction [28][29][30]. Screening of an oocyte-specific core histone H2A variant in fish was also performed [14].…”

“…Antibody specificity was demonstrated by Western blot using the expressing recombinant protein and the histone sample of eggs. Western blot detection followed the protocol previously published [27,39,40]. All chemicals were purchased from SigmaAldrich.…”