Identification of a potential fungal species by 18S rDNA for ligninases production

Abstract

Fungal species for ligninases production was investigated by 18S ribosomal DNA sequence analysis. Two primer sets were chosen to amplify a major part of the 18S rDNA, which resulted in intense PCR product of approximately 550-820 bp in size per sample. The results suggest that the 18S rDNA-based approach is a useful tool for identification of unknown potential fungal species for ligninases production. The isolated fungal species produces mainly manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related polyphenols. Time course studies showed that maximum production of ligninolytic enzymes MnP (64 IU L⁻¹), lignin peroxidase (26.35 IU L⁻¹), and laccase (5.44 IU L⁻¹), respectively, were achieved after 10 days of cultivation under optimum conditions. Furthermore, the biological decolorization of Remazol Brilliant Blue R dye following 10 days of cultivation was 94 %. NCBI BLAST was used to search for closest matched sequences in the GenBank database and based on sequence homology the first BLAST hit was Dothioraceae sp. LM572 with accession number EF060858.1.

Lignin as a green and multifunctional alternative to phenol for resin synthesis

“…130 Sulfur-mediated demethylation can remove one or two ortho methyl groups to produce a phenolic hydroxyl group, resulting in enhanced reactivity in subsequent LPR synthesis. 109…”

“…108 Furthermore, the incorporation of lignin directly into phenolic resin to replace the phenol limits the percentage replacement due to the high molecular weight and lower aliphatic hydroxyl numbers of the lignin. [40][41][42] In order to enhance the reactivity of lignin, lignin modification strategies have been reported such as hydroxymethylation, 109,110 phenolation, 111,112 demethylation, and demethoxylation (Fig. 4 and Table 1).…”

Trametes villosa Lignin Peroxidase (TvLiP): Genetic and Molecular Characterization

“…Discoloration of dyes (such as RBBR) added to a medium in which ligninolytic fungi are grown occurs as a result of the expression of lignin-modifying peroxidase enzymes [46]. The RBBR assay was used as a qualitative test to eliminate culture media that apparently did not induce the ligninolytic enzymes expression.…”

Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass

“…including laccase, manganese peroxidase (MnP), and lignin peroxidase (LiP) (Ferhan et al 2013). Ligninolytic enzymes are produced during secondary metabolism.…”