Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Abstract

These results demonstrate that CYP3356A1 is related to the detoxification of imidacloprid, thiamethoxam, and β-cypermethrin in B. odoriphaga. Moreover, the study also increased our understanding of the molecular mechanisms of insecticide detoxification in this pest insect. © 2018 Society of Chemical Industry.

Functionally characterized arthropod pest and pollinator cytochrome P450s associated with xenobiotic metabolism

“…Five D. citri P450s (CYP4C67, CYP4DA1, CYP4C68, CYP4G70, CYP4DB1) are involved in imidacloprid (neonicotinoid) resistance (Killiny et al, 2014). Chen and colleagues depicted in several studies the relationship of B. odoriphaga CYP9b2, CYP49a1, CYP12b1 and CYP6FV12 and imidacloprid resistance (neonicotinoid), while CYP3356A1 has been related to imidacloprid, thiamethoxam (neonicotinoids) and b-cypermethrin (pyrethroid) resistance (Chen et al, 2018;Chen et al, 2019aChen et al, , 2019b (Table 2B).…”

Missing Nurse Bees—Early Transcriptomic Switch From Nurse Bee to Forager Induced by Sublethal Imidacloprid

“…In bumble bee (Bombus terrestris) workers, exposure to field-realistic imidacloprid concentrations (10 ppb) can induce a total of 405 DEGs with functions related to energy reserve metabolism, synaptic transmission, apoptotic processes, xenobiotic transport, and complement activation, and including P450 genes (Manjon et al, 2018); exposure to a sublethal dose of clothianidin (6.47 ppb) induces the differential expression of 55 genes, including three putative cytochrome P450 genes (Bebane et al, 2019;Colgan et al, 2019). In Bradysia odoriphaga, exposure to the LC 50 dose (4.08 mg/l) of imidacloprid for 24 h induces the differential expression of 674 unigenes with functions related to cytochrome P450, cell adhesion, axon guidance, and carbohydrate metabolic process (Chen et al, 2019). The effects of neonicotinoids on insect gene expression vary among different species of insects, but genes related to metabolism, detoxification (especially cytochrome P450), and neuron development/synaptic transmission seem to be commonly found.…”

Insecticide Resistance in the Brown Planthopper, Nilaparvata lugens (Stål): Mechanisms and Status in Asian Countries

“…The elevated activities of metabolic enzymes P450, esterases (EST) and glutathione S-transferases (GST) have been reported in several insect species resistant to insecticides (Bass & Field, 2011;Liang et al, 2018). Through the synergistic and enzymatic assay, increased activity of detoxifying P450 enzyme has been found in many insects in response to neonicotinoids insecticides (Chen, Shan, Liu, Shi, & Gao, 2019). Similarly, insecticide resistance of N. lugens against different insecticides is mostly controlled by increased enzyme activity, especially P450 enzyme.…”