Identification of a novel bipartite nuclear localization signal in the DNA polymerase of the betabaculovirus Pieris rapae granulovirus

Li, Pei; Wu, Lijuan; Chen, Guoqing

doi:10.1007/s00705-018-4115-0

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…As an essential gene for viral DNA replication, knockout of lef-11 will result in the stagnation of viral proliferation (Lin et al, 2002;Li et al, 2019). LEF-11 can self-interact to form homo-oligomers (dimers/tetramers), which is one of the necessary conditions for its function (Dong et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of LEF-11 acetylation modification on the regulation of baculovirus infection

Wu¹,

Shen²,

Gao³

et al. 2023

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Late expression factor 11 (LEF-11) is an essential protein in the regulation of Bombyx mori nucleopolyhedrovirus (BmNPV) DNA replication and late gene expression. Our recent quantitative analysis of protein acetylome revealed for the first time that LEF-11 can be acetylated at one lysine residue (K83) during viral infection, but the underlying mechanism is unclear. The acetylation level for K83 was down-regulated after 36 h post-infection by approximately 30%. To clarify the regulatory function of this modification, overlap PCR was used for site-specific mutagenesis for acetylated (K83Q) or deacetylated (K83R) mimic mutants of LEF-11. The results of viral titration and quantitative polymerase chain reaction showed that after K83 acetylation, budding virion production and the viral genome replication level were significantly upregulated. Meanwhile, the results of yeast two-hybrid (y2H) system confirmed that K83 deacetylation modification inhibited the interaction between LEF-11 and immediate early gene 1 (IE-1). In conclusion, the acetylation of LEF-11 at K83 might enhance the interaction with IE-1 in the host cell nucleus to promote viral DNA replication, and might be one of the antiviral strategies of the silkworm host. The host inhibits virus proliferation by deacetylating LEF-11.

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Section: Discussionmentioning

confidence: 99%

Effects of LEF-11 acetylation modification on the regulation of baculovirus infection

Wu¹,

Shen²,

Gao³

et al. 2023

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“…NLSs have also been detected on the DNApol of the beta baculovirus PiraGV. But unlike the classical dichotomous NLSs, mutation or deletion of any of the clusters of basic amino acids results in nuclear translocation failure, indicating an evolutionary divergence between nucleopolyhedroviruses (NPV) and granuloviruses (GV) [66]. Revealing the mechanism of co evolution between hosts and baculovirus is beneficial for humans to better control and "utilize" baculovirus.…”

Section: Summary and Future Prospectsmentioning

confidence: 99%

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Li,

Wang,

2024

Preprint

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Baculovirus is a type of virus with a double stranded, circular, and super helical DNA structure, whose transcription, replication and assembly of offspring virus occur in the nucleus of the cell. However, due to the natural barrier of the cellular nuclear membrane, macromolecular substances larger than 40-60 kDa cannot directly penetrate the nuclear membrane. Therefore, baculovirus have evolved various strategies to achieve transmembrane transport of viral proteins. This article attempts to review the recent literature on mechanisms by which the DNA baculoviruses of insects transport proteins into the infected host cell nucleus. In this article, we summarise the studies of nuclear localisation signals(NLSs) in baculovirus, which are involved in the classical nuclear import pathways and review the non-classical nuclear transport pathways independent on NLS derived from baculovirus. Moreover, during the protein nuclear transport process, viruses will regulate the nuclear pathway through protein modifications. Host structure changes and other methods will create a favorable environment for the nuclear transport of viral proteins.

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Microbial Source of Insect‐Toxic Proteins

Yadav

Mishra

2021

Bioprospecting of Microorganism‐Based Industrial Molecules

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Identification of a novel bipartite nuclear localization signal in the DNA polymerase of the betabaculovirus Pieris rapae granulovirus

Cited by 3 publications

References 30 publications

Effects of LEF-11 acetylation modification on the regulation of baculovirus infection

Effects of LEF-11 acetylation modification on the regulation of baculovirus infection

Molecular Mechanisms of Nuclear Import of Baculovirus Proteins

Microbial Source of Insect‐Toxic Proteins

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