2012
DOI: 10.1002/lt.22480
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Identification of a novel biomarker gene set with sensitivity and specificity for distinguishing between allograft rejection and tolerance

Abstract: Here we examined whether the expression of a novel immunoregulatory gene set could be used to predict outcomes in murine models of rapamycin-induced cardiac tolerance, spontaneous hepatic tolerance, and cardiac rejection. The expression of the immunoregulatory gene set was assessed with the GeXP multiplex reverse-transcription polymerase chain reaction (RT-PCR) analysis system, and it was correlated to the pathological and biochemical parameters of the allografts. In rejecting cardiac grafts, the increased exp… Show more

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Cited by 46 publications
(53 citation statements)
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“…In transplantation, administration of soluble FGL2 was shown to double skin allograft survival but did not result in long-term survival [12]. Recently, FGL2 together with Foxp3 and killer cell lectin-like receptor G1, were shown to be over-expressed in models of liver and cardiac transplantation tolerance [29]. Mice deficient in FGL2 develop autoimmune glomerulonephritis [28].…”
Section: Discussionmentioning
confidence: 99%
“…In transplantation, administration of soluble FGL2 was shown to double skin allograft survival but did not result in long-term survival [12]. Recently, FGL2 together with Foxp3 and killer cell lectin-like receptor G1, were shown to be over-expressed in models of liver and cardiac transplantation tolerance [29]. Mice deficient in FGL2 develop autoimmune glomerulonephritis [28].…”
Section: Discussionmentioning
confidence: 99%
“…To determine if there was a sustained difference in the cellular regulatory levels between ALS þ CsA and ALS þ CsA þ hIL-2/Fc treated recipients, we examined the gene expression profile of various effector and regulatory markers using real-time PCR at day 35 posttransplantation, 2 weeks after cessation of the treatment, as these biomarkers have been shown to correlate with rejection versus tolerance induction (33). A striking difference was demonstrated in the relative expression of FoxP3 when compared to effector cell gene markers between the ALS þ CsA and ALS þ CsA þ hIL-2/Fc treated groups (Figure 4b).…”
Section: Discussionmentioning
confidence: 99%
“…The administration of EPA to CBA mice transplanted with B10 hearts exhibited a permanent survival ( Figure 1). As previously reported, 12 the cardiac allografts exhibited moderate to severe rejection on day 8 after transplant as assessed according to a standard cardiac biopsy grading system. Additionally, the cardiac allografts treated with EPA on day 100 after transplant did not display any signs of rejection.…”
Section: Histologic Analysis Of Cardiac Allograftsmentioning
confidence: 65%